A5065849527- Electrochemical sensors and biosensors
- Electrochemical Analysis and Applications
- Metalloenzymes and iron-sulfur proteins
- Microbial Fuel Cells and Bioremediation
- Analytical Chemistry and Sensors
- Microbial metabolism and enzyme function
- Electrocatalysts for Energy Conversion
- CO2 Reduction Techniques and Catalysts
- Microbial Metabolic Engineering and Bioproduction
- Advanced Chemical Sensor Technologies
- Amino Acid Enzymes and Metabolism
- Enzyme-mediated dye degradation
- Lipid Membrane Structure and Behavior
- Enzyme Catalysis and Immobilization
- Ammonia Synthesis and Nitrogen Reduction
- Neuroscience and Neural Engineering
- Photoreceptor and optogenetics research
- Advanced battery technologies research
- Supercapacitor Materials and Fabrication
- Microfluidic and Capillary Electrophoresis Applications
- Toxic Organic Pollutants Impact
- Fuel Cells and Related Materials
- Dyeing and Modifying Textile Fibers
- Advanced Electron Microscopy Techniques and Applications
- Biochemical Acid Research Studies
Kyoto University
2021-2025
Among the various types of enzyme-based biosensors, sensors utilizing enzymes capable direct electron transfer (DET) are recognized as most ideal. However, only a limited number redox DET with electrodes, that is, dehydrogenases harboring subunit or domain functions specifically to accept electrons from cofactor catalytic site and external acceptor. Such subunits domains act built-in mediators for between electrodes; consequently, such enable electrodes designated DET-type enzymes. fall into...
The efficient utilization of biomass fuels is a critical component sustainable energy economy. Via respiration, acetic acid bacteria can oxidize ethanol into using membrane-bound alcohol and aldehyde dehydrogenases (ADH AlDH, respectively). Focusing on the ability these enzymes to interact directly electrically with electrode materials, we constructed mediatorless bioanode for oxidation based direct electron transfer (DET)-type bienzymatic cascade by ADH AlDH. three-dimensional structural...
Flavin adenine dinucleotide-dependent d-fructose dehydrogenase (FDH) from Gluconobacter japonicus NBRC3260, a membrane-bound heterotrimeric flavohemoprotein capable of direct electron transfer (DET)-type bioelectrocatalysis, was investigated the perspective structural biology, bioelectrochemistry, and protein engineering. DET-type reactions offer several benefits in biomimetics (e.g., biofuel cells, bioreactors, biosensors) owing to their mediator-less configuration. FDH provides an intense...
Herein, we report a scalable benchtop electrode fabrication method to produce highly sensitive and flexible third-generation fructose dehydrogenase amperometric biosensors based on water-dispersed 0D-nanomaterials. The electrochemical platform was fabricated via Stencil-Printing (StPE) insulated xurography. Carbon black (CB) mesoporous carbon (MS) were employed as 0D-nanomaterials promoting an efficient direct electron transfer (DET) between (FDH) the transducer. Both nanomaterials prepared...
Copper efflux oxidase (CueO) is involved in copper homeostasis Escherichia coli by catalyzing the oxidation of cuprous ion (Cu+) to cupric (Cu2+). CueO has been studied as a direct electron transfer (DET)-type bioelectrocatalyst owing its high dioxygen-reducing activity. Our previous study demonstrated reductive inactivation DET-type bioelectrocatalysis presence Cu2+, which was known facilitate substrate solution. Considering structural data, we hypothesized that sixth-bound (Cu6) induced...
Membrane-bound heterotrimeric d-fructose dehydrogenase (FDH) from Gluconobacter japonicus exhibits distinct direct electron transfer (DET)-type bioelectrocatalytic activity. FDH contains three heme c moieties (heme 1c, 2c, and 3c), the two downsized variants lacking 1c moiety (Δ1c FDH) or 2c (Δ1c2c were constructed in our previous research. Recently, we elucidated entire structure of FDH, enabling a structural perspective mechanistic discussions. In this study, quantitatively analyzed...
Herein, a strategy to stamp laser-produced reduced graphene oxide (rGO) onto flexible polymers using only office-grade tools, namely, roll-to-roll thermal stamping, is proposed, proving for the first time its effectiveness direct bioelectrocatalysis. This straightforward, scalable, and low-cost approach allows us overcome limits of integration laser-induced rGO-films in bioanalytical devices. Laser-produced rGO has been thermally stamped (TS) different polymeric substrates (PET, PVC, EVA)...
Tungsten-containing formate dehydrogenase from Methylorubrum extroquens AM1 (FoDH1)-a promising biocatalyst for the interconversion of carbon dioxide/formate and nicotine adenine dinucleotide (NAD+)/NADH redox couples-was investigated using structural biology bioelectrochemistry. FoDH1 is reported to be an enzyme that can realize "direct electron transfer (DET)-type bioelectrocatalysis." However, its 3-D structure, electrode-active sites, (ET) pathways remain unclear. The ET were...
Ethanol skin gas can be used to determine auto-brewery syndrome (drunken disease), blood alcohol levels, and/or a body state of alcoholism. Considering the limitations continuous monitoring...
The possibility to print electronics by means of office tools has remarkedly increased the design affordable and robust point-of-care/need devices. However, conductive inks suffer from low electrochemical rheological performances limiting their applicability in biosensors. Herein, a fast CO
Some oxidoreductases can communicate directly and electrically with electrodes; this process is called direct electron transfer (DET)-type bioelectrocatalysis. Understanding its detailed mechanisms essential for developing improving DET-based bioelectrochemical devices. In study, we investigated the pH dependence of kinetic thermodynamic characteristics a variant an aldehyde dehydrogenase (ALDH) without cytochrome c subunit (ΔC_ALDH) compared it that wild-type recombinant ALDH (rALDH). Owing...
When a test substance is poorly water-soluble, it can be adsorbed onto silica gel to facilitate dispersibility in ready biodegradability test. To uniformly adsorb the gel, dissolved solvent and then mixed with gel. It desirable for completely evaporate afterward. In this study, we identified n-hexane as suitable purpose. Furthermore, through fluorescence observation, revealed that adhered activated sludge flocs. This thought improve contact between microorganisms, thereby accelerating biodegradation.
We investigated whether various modified cyclodextrins (CDs) and emulsifiers could be applied as dispersing agents in ready biodegradability tests of poorly water-soluble substances. Trimethylated α-, β-, γ-CDs partially methylated β-CD were not biodegraded the test period but accelerated biodegradation octabenzone anthraquinone. The process by which trimethylated enhance substances has been uncovered. These CDs create inclusion complexes with substances, then coalesce into larger...
All-solid-state NO
Flavin adenine dinucleotide (FAD)-dependent fructose dehydrogenase (FDH) from Gluconobacter japonicus NBRC3260, a membrane-bound flavohemoprotein capable of direct electron transfer (DET)-type bioelectrocatalysis, was investigated the viewpoints structural biology and bioelectrochemistry. As FDH provides strong DET-type catalytic signal, extensive research has been conducted. Structural analysis using cryo-electron microscopy (cryo-EM) single-particle revealed entire structure. The (ET)...