- Electrochemical sensors and biosensors
- Microbial Fuel Cells and Bioremediation
- Metalloenzymes and iron-sulfur proteins
- Microfluidic and Capillary Electrophoresis Applications
- Analytical Chemistry and Sensors
- Advanced biosensing and bioanalysis techniques
- Metabolism and Genetic Disorders
- Electrochemical Analysis and Applications
- Ammonia Synthesis and Nitrogen Reduction
- Supercapacitor Materials and Fabrication
Kyoto University
2023-2024
Among the various types of enzyme-based biosensors, sensors utilizing enzymes capable direct electron transfer (DET) are recognized as most ideal. However, only a limited number redox DET with electrodes, that is, dehydrogenases harboring subunit or domain functions specifically to accept electrons from cofactor catalytic site and external acceptor. Such subunits domains act built-in mediators for between electrodes; consequently, such enable electrodes designated DET-type enzymes. fall into...
Herein, we report a scalable benchtop electrode fabrication method to produce highly sensitive and flexible third-generation fructose dehydrogenase amperometric biosensors based on water-dispersed 0D-nanomaterials. The electrochemical platform was fabricated via Stencil-Printing (StPE) insulated xurography. Carbon black (CB) mesoporous carbon (MS) were employed as 0D-nanomaterials promoting an efficient direct electron transfer (DET) between (FDH) the transducer. Both nanomaterials prepared...
Herein, a strategy to stamp laser-produced reduced graphene oxide (rGO) onto flexible polymers using only office-grade tools, namely, roll-to-roll thermal stamping, is proposed, proving for the first time its effectiveness direct bioelectrocatalysis. This straightforward, scalable, and low-cost approach allows us overcome limits of integration laser-induced rGO-films in bioanalytical devices. Laser-produced rGO has been thermally stamped (TS) different polymeric substrates (PET, PVC, EVA)...
The possibility to print electronics by means of office tools has remarkedly increased the design affordable and robust point-of-care/need devices. However, conductive inks suffer from low electrochemical rheological performances limiting their applicability in biosensors. Herein, a fast CO
The interest in the use of redox enzymes for construction efficient biodevices has grown to achieve an environmentally friendly society. “Bioelectrocatalysis,” which enzymatic reaction and electrode reactions are coupled, is a fundamental technology various electrochemical biomimetics (e.g., biosensors, biofuel cells, bioreactors). In particular, enzyme directly shuttles electrons without any external electron mediators called direct transfer (DET)-type reaction. Thanks its mediator-less...