A5052173682- Multiple Myeloma Research and Treatments
- Cell Adhesion Molecules Research
- CAR-T cell therapy research
- Cancer Genomics and Diagnostics
- Angiogenesis and VEGF in Cancer
- Immunotherapy and Immune Responses
- SARS-CoV-2 and COVID-19 Research
- Nanoplatforms for cancer theranostics
- Chemokine receptors and signaling
- Single-cell and spatial transcriptomics
- Monoclonal and Polyclonal Antibodies Research
- T-cell and B-cell Immunology
- Chronic Lymphocytic Leukemia Research
- Cancer, Hypoxia, and Metabolism
- Immune Cell Function and Interaction
- vaccines and immunoinformatics approaches
- Inflammatory mediators and NSAID effects
- Bacteriophages and microbial interactions
- Cancer Immunotherapy and Biomarkers
- Immune cells in cancer
- Viral-associated cancers and disorders
- Hematological disorders and diagnostics
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- HIV Research and Treatment
- Retinoids in leukemia and cellular processes
Harvard University
2021-2025
Broad Institute
2021-2025
Kyoto University
2018-2025
Dana-Farber Cancer Institute
2021-2025
Massachusetts Institute of Technology
2021-2025
Kansai Electric Power (Japan)
2021-2023
RIKEN Center for Integrative Medical Sciences
2021-2023
Osaka Red Cross Hospital
2013-2016
Natural killer (NK) cells lyse invading tumor to limit metastatic growth in the lung, but how some cancers evade this host protective mechanism establish a growing lesion is unknown. Here, we have combined ultra-sensitive bioluminescence imaging with intravital two-photon microscopy involving genetically encoded biosensors examine question. NK eliminated disseminated from lung within 24 hr of arrival, not thereafter. Intravital dynamic revealed that 50% NK-tumor cell encounters lead death...
Abstract We are amid the historic coronavirus infectious disease 2019 (COVID-19) pandemic. Imbalances in accessibility of vaccines, medicines, and diagnostics among countries, regions, populations, those war crises, have been problematic. Nanobodies small, stable, customizable, inexpensive to produce. Herein, we present a panel nanobodies that can detect spike proteins five SARS-CoV-2 variants concern (VOCs) including Omicron. Here show via ELISA, lateral flow, kinetic, flow cytometric,...
Multicentric Castleman's disease is a systemic inflammatory disorder characterized by lymphadenopathy and excessive interleukin-6 production. A unique clinicopathologic variant of multicentric disease, TAFRO (i.e., thrombocytopenia, anasarca, fever, renal failure or reticulin fibrosis, organomegaly) syndrome, was recently proposed in Japan. Despite the successful use anti-interleukin-6 therapy some patients with not all achieve remission. The pathophysiological etiology suitable therapeutic...
Waldenstrom's Macroglobulinemia (WM) is an IgM-secreting bone marrow (BM) lymphoma that preceded by asymptomatic state (AWM). To dissect tumor-intrinsic and immune mechanisms of progression, we perform single-cell RNA-sequencing on 294,206 BM tumor cells from 30 patients with AWM/WM, 26 Smoldering Myeloma, 23 healthy donors. Despite their early stage, AWM present extensive dysregulation, including in normal B cells, disease-specific hallmarks. Patient T NK show systemic hypo-responsiveness...
ABSTRACT Background DNA cytosine deaminase APOBEC3B (A3B) is one of the endogenous sources somatic mutations in many types human cancers and associated with tumor progression rather than tumorigenesis. However, it remains uncertain whether APOBEC3B‐induced accelerate or not. In this paper, we established a mouse model A3B overexpression investigated introduction accelerates development Tp53 hemizygous mice. Methods We transgenic by microinjection selected which has only transgene genomic...
Introduction: Multiple Myeloma (MM) is a bone marrow (BM) plasma cell malignancy that preceded by asymptomatic stages; Monoclonal Gammopathy of Undetermined Significance (MGUS) and Smoldering (SMM). Despite their early stage, patients with MGUS/SMM exhibit immune dysregulation. Understanding how dysregulation evolves tumor cells interact to promote progression MM may help predict intercept it. Methods: We performed single-cell RNA/BCR/TCR-seq (10X Genomics) on ∼6 million from 877 samples,...
Prostaglandin E2 (PGE2) promotes tumor progression through evasion of antitumor immunity. In stark contrast to cyclooxygenase-dependent production PGE2, little is known whether PGE2 secretion regulated within tissues. Here, we show that VEGF-dependent release thromboxane A2 (TXA2) triggers Ca2+ transients in cells, culminating and subsequent immune the early stages tumorigenesis. caused cPLA2 activation triggered arachidonic acid cascade. were monitored as surrogate marker secretion....
APOBEC3B (A3B) is a cytosine deaminase that converts to uracil in single-stranded DNA. Cytosine-to-thymine and cytosine-to-guanine base substitution mutations trinucleotide motifs (APOBEC mutational signatures) were found various cancers including lymphoid hematological malignancies such as multiple myeloma A3B has been shown be an enzymatic source of those cancers. Although the importance being increasingly recognized, it unclear how expression regulated cancer cells well normal cells. To...
Extracellular signal-regulated kinase (ERK) plays critical roles in T cell development the thymus. Nevertheless, dynamics of ERK activity and role regulating thymocyte motility remain largely unknown due to technical limitations. To visualize thymocytes, we here developed knockin reporter mice expressing a Förster/fluorescence resonance energy transfer (FRET)-based biosensor for from ROSA26 locus. Live imaging thymocytes isolated revealed that regulates subtype-specific manner. Negative...
Abstract DNA cytosine deaminase APOBEC3B (A3B) is an endogenous source of mutations in many human cancers, including multiple myeloma. A3B proteins form catalytically inactive high molecular mass (HMM) complexes nuclei, however, the regulatory mechanisms activity HMM are still unclear. Here, we performed spectrometry analysis A3B-interacting from nuclear extracts myeloma cell lines and identified 30 putative interacting proteins. These involved RNA metabolism, binding, mRNA splicing,...
Fibrosing mediastinitis is rare. One type of this disease idiopathic fibrosing mediastinitis. It necessary to rule out malignancy in order accurately diagnose We herein report a case anaplastic large cell lymphoma diagnosed three months after preliminary diagnosis Glucocorticoid therapy was not successful controlling progression. Immediately initiating chemotherapy for lymphoma, the patient's symptoms improved dramatically and mediastinal lesion decreased size. Although few similar cases...
Abstract Background:The immune system is critical for response and resistance in all cancer types therapies. Patients with Smoldering Multiple Myeloma (SMM) have altered cell composition the bone marrow (BM). However, little known about role of BM spatial profiling patients treated checkpoint inhibitors MM. Methods: We performed imaging mass cytometry trephine biopsies (n=15; pre-treatment n=7 post-treatment n=8) from high-risk SMM enrolled a phase II trial nivolumab combination lenalidomide...