A5070212943- Microtubule and mitosis dynamics
- ECG Monitoring and Analysis
- Chromosomal and Genetic Variations
- Genomics and Chromatin Dynamics
- Visual perception and processing mechanisms
- Cardiac electrophysiology and arrhythmias
- RNA Research and Splicing
- Reproductive Biology and Fertility
- Dialysis and Renal Disease Management
- Health Promotion and Cardiovascular Prevention
- Cancer-related Molecular Pathways
- Mitochondrial Function and Pathology
- Ubiquitin and proteasome pathways
- DNA Repair Mechanisms
- Cardiac pacing and defibrillation studies
- Cardiac Arrhythmias and Treatments
- RNA and protein synthesis mechanisms
- Health and Wellbeing Research
- Rheumatoid Arthritis Research and Therapies
- Cellular transport and secretion
- COVID-19 and Mental Health
- EEG and Brain-Computer Interfaces
- Chronic Disease Management Strategies
- Memory and Neural Mechanisms
- Muscle and Compartmental Disorders
Osaka University
2015-2024
St. Marianna University School of Medicine
1999-2024
Ube Frontier University
2018-2024
Japanese Red Cross Fukuoka Hospital
2023-2024
Whitehead Institute for Biomedical Research
2014-2021
Fukuoka Dental College
2020-2021
ORCID
2021
Kyushu University
2016-2020
Massachusetts Institute of Technology
2014-2017
Matsuyama Red Cross Hospital
2014-2016
<b>Objective:</b> To estimate the disease activity score (DAS)28-C-reactive protein (CRP) threshold values that correspond to DAS28-erythrocyte sedimentation rate (ESR) for remission, low and high in patients with rheumatoid arthritis. <b>Methods:</b> DAS28 data were analysed using a large observational study (Institute of Rheumatology Rheumatoid Arthritis) database 6729 Firstly, relationship between DAS28-ESR DAS28-CRP was analysed. Secondly, best trade-off each calculated receiver...
Maturation/M-phase-promoting factor is the universal inducer of M-phase in eukaryotic cells. It currently accepted that M-phase-promoting identical to kinase cyclin B–Cdk1. Here we show B–Cdk1 and are not fact synonymous. Instead, contains at least two essential components: another kinase, Greatwall kinase. In absence undetectable oocyte cytoplasm even though fully active, whereas activity restored when added back. Although excess amount alone, but can induce nuclear envelope breakdown,...
Kinetochore is an essential protein complex required for accurate chromosome segregation. The constitutive centromere-associated network (CCAN), a subcomplex of the kinetochore, associates with centromeric chromatin and provides platform kinetochore assembly. CCAN CENP-C thought to be central hub centromere/kinetochore organization. However, role in assembly needs elucidated. Here, we demonstrate that both CCAN-binding domain C-terminal region includes Cupin are necessary sufficient chicken...
<b>Objective:</b> To determine the risk for tuberculosis infection in patients with rheumatoid arthritis before anti-cytokine era Japan. <b>Patients and methods:</b> A database of a single-institute-based large observational cohort study at Institute Rheumatology, Tokyo Women's Medical University, Tokyo, Japan, was analysed. Information on history collected by patient self-reporting during April October 2003. The age-adjusted incidence rate relative were investigated. <b>Results:</b> Among...
The kinetochore is essential for faithful chromosome segregation during mitosis. To form a functional kinetochore, constitutive centromere-associated network (CCAN) proteins are assembled on the centromere chromatin that contains centromere-specific histone CENP-A. CENP-C, CCAN protein, directly interacts with CENP-A nucleosome to nucleate structure. As CENP-C hub protein assembly, it critical address how CENP-A–CENP-C interaction regulated cell cycle progression. this question, we...
Abstract To form functional kinetochores, CENP-C and CENP-T independently recruit the KMN (Knl1C, Mis12C, Ndc80C) network onto kinetochores. clarify functions of on CENP-T, we evaluated its roles in chicken DT40 cell lines lacking CENP-C-KMN interaction. By analyzing mutants both CENP-T-Mis12C CENP-C-Mis12C interactions, demonstrated that Knl1C Mis12C (KM) play critical cohesion sister chromatids or recruitment spindle checkpoint proteins Two copies Ndc80C (N-N) exist via direct binding....
<h3>Objectives:</h3> The aim of this study was to examine the effect efficient management rheumatoid arthritis (RA) in relation disability levels a large cohort patients with RA over period 3 years. <h3>Methods:</h3> We studied 2775 who had continuous enrolment for at least years from 7511 enrolled an observational (Institute Rheumatology, Rheumatoid Arthritis (IORRA)) October 2000 April 2005. 28-joint Disease Activity Scores (DAS28) were calculated 6 month intervals all and value <2.6...
Entry into M phase is governed by cyclin B–Cdk1, which undergoes both an initial activation and subsequent autoregulatory activation. A key part of the B–Cdk1–dependent inhibition protein phosphatase 2A (PP2A)–B55, antagonizes B–Cdk1. Greatwall kinase (Gwl) believed to be essential for because Gwl activated downstream B–Cdk1 phosphorylate activate α-endosulfine (Ensa)/Arpp19, inhibitor PP2A-B55. However, becomes fully in some conditions lacking Gwl, yet how this accomplished remains unclear....
The Drosophila Pan Gu (PNG) kinase complex regulates hundreds of maternal mRNAs that become translationally repressed or activated as the oocyte transitions to an embryo. In a previous paper (Hara et al., 2017), we demonstrated PNG activity is under tight developmental control and restricted this transition. Here, examination specificity showed it be Thr-kinase yet lacking clear phosphorylation site consensus sequence. An unbiased biochemical screen for substrates identified conserved...
The oocyte-to-embryo transition involves extensive changes in mRNA translation, regulated Drosophila by the PNG kinase complex whose activity we show here to be under precise developmental control. Despite presence of catalytic subunit and PLU GNU activating subunits mature oocyte, is phosphorylated at Cyclin B/CDK1sites unable bind PLU. In vitro phosphorylation CyclinB/CDK1 blocks activation PNG. Meiotic completion promotes dephosphorylation regulate translation. critical regulatory effect...
The cell cycle in oocytes generally arrests at a particular meiotic stage to await fertilization. This arrest occurs metaphase of meiosis II (meta-II) frog and mouse, G1 phase after completion starfish. Despite this difference the phase, both depend on same Mos-MAPK (mitogen-activated protein kinase) pathway, indicating that relies downstream effectors. Immediately MAPK, Rsk (p90 ribosomal S6 kinase, p90(Rsk)) is required for meta-II arrest. However, mouse challenges requirement, no effector...
A key event in the oocyte-to-embryo transition is start of embryonic mitotic cell cycle. Prior to this start, cycle oocytes generally arrested at a particular stage during meiosis, and meiotic arrest released by fertilization. However, it remains unclear how release from implicated To elucidate link, we have used starfish eggs, which G1 phase occurs after completion meiosis if mature are not fertilized, fertilization simply directs The known rely on Mos-MAPK-Rsk (p90 ribosomal S6 kinase)...
Gene downregulation by antisense morpholino oligonucleotides (MOs) is achieved either hybridization around the translation initiation codon or targeting splice donor site. In present study, an MO method introduced that uses a 25-mer against region at least 40-nt upstream from poly(A) tail junction in 3′-untranslated (UTR) of maternal mRNA. The removed and blocked zebrafish cdk9 (zcdk9) mRNA translation, showing functional mimicry between miRNA MO. A PCR-based assay revealed MO-mediated...
Abstract The kinetochore is essential for faithful chromosome segregation during mitosis and assembled through dynamic processes involving numerous proteins. Various experimental strategies have been used to understand assembly processes. Fluorescence recovery after photobleaching (FRAP) analysis also a useful strategy revealing the dynamics of assembly. In this study, we introduced fluorescence protein-tagged protein cDNAs into each endogenous locus performed FRAP analyses in chicken DT40...
Background Outflow obstruction, a common complication in patients with peritoneal dialysis (PD), usually results unnecessary catheter removal or replacement. This study describes modified simple method of anchoring PD on the anterior wall without using laparoscopic system (peritoneal anchor technique, PWAT). Methods We performed retrospective cohort consecutive insertions, and compared survival rate between traditional PWAT. The was used 54 cases PWAT 17 cases. primary endpoint occurrence...