A5079061958- Microtubule and mitosis dynamics
- Reproductive Biology and Fertility
- Nuclear Structure and Function
- Cellular Mechanics and Interactions
- Epigenetics and DNA Methylation
- RNA Research and Splicing
- Microfluidic and Bio-sensing Technologies
- Antimicrobial Peptides and Activities
- CRISPR and Genetic Engineering
- Pluripotent Stem Cells Research
- Industrial Automation and Control Systems
- Transgenic Plants and Applications
- Structural Engineering and Vibration Analysis
- Animal Genetics and Reproduction
- Prenatal Screening and Diagnostics
- Multimodal Machine Learning Applications
- Misinformation and Its Impacts
- Advancements in PLL and VCO Technologies
- Structural Load-Bearing Analysis
- Micro and Nano Robotics
- Advanced Neuroimaging Techniques and Applications
- Biochemical and Structural Characterization
- Ocular Disorders and Treatments
- Reproductive biology and impacts on aquatic species
- Agricultural Engineering and Mechanization
Kubota (Japan)
2024
RIKEN Center for Biosystems Dynamics Research
2020-2021
Osaka University
2016-2021
European Molecular Biology Laboratory
2013-2020
European Bioinformatics Institute
2011-2019
Microbial Chemistry Research Foundation
2019
European Molecular Biology Laboratory
2010-2018
RIKEN Center for Computational Science
2018
Industrial Research Institute of Ishikawa
2014
Tokyo Institute of Technology
2002-2010
Gene-expression analysis studies from Schultz et al. estimate that more than 2,300 genes in the mouse genome are expressed predominantly male germ line. As of their 2003 publication [Schultz N, Hamra FK, Garbers DL (2003) Proc Natl Acad Sci USA 100(21):12201-12206], functions majority these testis-enriched during spermatogenesis and fertilization were largely unknown. Since study by al., functional hundreds reproductive-tract-enriched have been performed, but there remain many for which...
Surface contraction waves (SCWs) in oocytes and embryos lead to large-scale shape changes coupled cell cycle transitions are spatially coordinated with the axis. Here, we show that SCWs starfish oocyte generated by a traveling band of myosin II-driven cortical contractility. At front band, contractility is activated removal cdk1 inhibition RhoA/RhoA kinase/myosin II signaling module, while at rear, switched off negative feedback originating downstream RhoA kinase. The SCW's directionality...
The nucleus of oocytes (germinal vesicle) is unusually large and its nuclear envelope (NE) densely packed with pore complexes (NPCs) that are stockpiled for embryonic development. We showed breakdown this specialized NE mediated by an Arp2/3-nucleated F-actin 'shell' in starfish oocytes, contrast to microtubule-driven tearing mammalian fibroblasts. Here, we address the mechanism F-actin-driven rupture correlated live-cell, super-resolution electron microscopy. show actin nucleated within...
Abstract Chromosome segregation errors in oocytes lead to the production of aneuploid eggs, which are leading cause pregnancy loss and several congenital diseases such as Down syndrome. The frequency chromosome increases with maternal age, especially at a late stage reproductive life. How aging various life stages affects differently remains poorly understood. In this study, we describe aging‐associated changes transcriptome profile mouse throughout Our single‐oocyte comprehensive RNA...
Spread of Fake news is a major challenge in this digital age where information shared through various means like internet articles or social media. Since the proliferation fake on rise, spread misinformation concern. Spreading have wide repercussions as it has wider reach due to straightforward access and rapid dispersion. This paper proposes Deep Learning approach along with Natural Language Processing Techniques solution for detection. We used ISOT dataset train test our model GloVe word...
Capture of each and every chromosome by spindle microtubules is essential to prevent loss aneuploidy. In somatic cells, astral search capture chromosomes forming lateral attachments kinetochores. However, this mechanism alone insufficient in large oocytes. We have previously shown that a contractile F-actin network additionally required collect scattered the 70-µm starfish oocyte nucleus. How F-actin–driven coordinated with microtubule remained unknown. Here, we show after nuclear envelope...
Zygotes require two accurate sets of parental chromosomes, one each from the mother and father, to undergo normal embryogenesis. However, upon egg–sperm fusion in vertebrates, zygote has three sperm egg. The therefore eliminates set maternal chromosomes (but not paternal chromosomes) into polar body through meiosis, but how are protected meiosis been unclear. Here we report that RanGTP F-actin dynamics prevent proximity chromosomes. prevents localization Juno CD9, egg membrane proteins...
The cell cycle in oocytes generally arrests at a particular meiotic stage to await fertilization. This arrest occurs metaphase of meiosis II (meta-II) frog and mouse, G1 phase after completion starfish. Despite this difference the phase, both depend on same Mos-MAPK (mitogen-activated protein kinase) pathway, indicating that relies downstream effectors. Immediately MAPK, Rsk (p90 ribosomal S6 kinase, p90(Rsk)) is required for meta-II arrest. However, mouse challenges requirement, no effector...
A key event in the oocyte-to-embryo transition is start of embryonic mitotic cell cycle. Prior to this start, cycle oocytes generally arrested at a particular stage during meiosis, and meiotic arrest released by fertilization. However, it remains unclear how release from implicated To elucidate link, we have used starfish eggs, which G1 phase occurs after completion meiosis if mature are not fertilized, fertilization simply directs The known rely on Mos-MAPK-Rsk (p90 ribosomal S6 kinase)...
There are over 200 genes that predicted to be solely expressed in the oocyte and ovary, thousands more have expression patterns female reproductive tract. Unfortunately, many of their physiological functions, such as roles oogenesis or fertilization, yet elucidated. Previous knockout (KO) mice studies proven were once thought essential for fertility dispensable vivo. Therefore, it is extremely important confirm all before spending immense time studying them vitro. To do this, our laboratory...
Initiation of DNA replication in eukaryotic cells is controlled through an ordered assembly protein complexes at origins. The molecules involved this process are well conserved but diversely regulated. Typically, initiation regulated response to developmental events multicellular organisms. Here, we elucidate the regulation first S phase embryonic cell cycle after fertilization. Unless fertilization occurs, Mos-MAPK-p90Rsk pathway causes G1-phase arrest completion meiosis starfish eggs....
QBRICK, FRAS1, and FREM2 compose a family of extracellular matrix proteins characterized by twelve consecutive CSPG repeats single or multiple Calx-β motifs. Dysfunction these have been associated with Fraser syndrome, which is malformation skin, eyes, digits, kidneys. FREM3 another member the 12-CSPG protein family. However, it remains unknown whether genetic dysfunction also causes syndrome developmental disorder. Here we investigated Frem3 mutant mouse line generated CRISPR/Cas9-mediated...
The EMBO workshop 'Oocyte maturation and fertilization: lessons from canonical emerging models' was held at the Oceanologic Observatory of Banyuls in France June 2013 organized by Anne-Marie Geneviere, Olivier Haccard, Peter Lenart Alex McDougall. A total 78 participants shared their research on germline formation, oocyte development, sperm, fertilization early development. Here, we report highlights this meeting.
We have constructed a visual anthropomorphous agent system, called the software (VSA). The VSA has texture-mapped realistic human facial image and moves his/her face with expression in real-time. Also, it can recognize speech dialogue of user respond synthesized voice sentence by synchronizing mouth movement. This type multi-modal system may become very important technology for advanced interfaces near future. However, because our earlier was designed to fit specific application, had been...
Abstract The nucleus of oocytes, traditionally referred to as the germinal vesicle, is unusually large and its nuclear envelope (NE) densely packed with pore complexes (NPCs) stockpiled for embryonic development. We have shown that breakdown this specialized NE during meiosis starfish oocytes mediated by an Arp2/3-nucleated F-actin ‘shell’, in contrast microtubule-driven tearing somatic cells. detailed mechanism how cytoskeletal forces disrupt remains poorly understood any system. Here, we...
The paper illustrates the project for a structural glass walkway that will connect two important historical buildings in Pisa, Palazzo delle Logge di Banchi and Pretorio, which are currently used as administrative offices by City Council. entire is developed with particular attention to conservation of those buildings, idea build transparent footbridge does not distract people’s from surrounding buildings. pedestrian has span 9.15 metres over main commercial street Pisa. In order combine...