A5090860200- Reproductive Biology and Fertility
- Epigenetics and DNA Methylation
- Cancer-related gene regulation
- Nuclear Structure and Function
- CRISPR and Genetic Engineering
- Geology and Paleoclimatology Research
- RNA Research and Splicing
- Animal testing and alternatives
- Methane Hydrates and Related Phenomena
- Renal and related cancers
- Prenatal Screening and Diagnostics
- Groundwater and Isotope Geochemistry
- bioluminescence and chemiluminescence research
- Molecular Biology Techniques and Applications
- Chromatin Remodeling and Cancer
- Animal Genetics and Reproduction
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Genomics and Chromatin Dynamics
- DNA Repair Mechanisms
- Pluripotent Stem Cells Research
Kindai University
2021-2024
University of the Ryukyus
2012
Zygotes require two accurate sets of parental chromosomes, one each from the mother and father, to undergo normal embryogenesis. However, upon egg–sperm fusion in vertebrates, zygote has three sperm egg. The therefore eliminates set maternal chromosomes (but not paternal chromosomes) into polar body through meiosis, but how are protected meiosis been unclear. Here we report that RanGTP F-actin dynamics prevent proximity chromosomes. prevents localization Juno CD9, egg membrane proteins...
Histone H3 lysine36 dimethylation (H3K36me2) is generally distributed in the gene body and euchromatic intergenic regions. However, we found that H3K36me2 enriched pericentromeric heterochromatin some mouse cell lines. We here revealed mechanism of targeting H3K36me2. Among several H3K36 methyltransferases, NSD2 was responsible for inducing heterochromatic Depletion overexpression analyses NSD2-associating proteins recruitment to mediated through imitation switch (ISWI) chromatin remodeling...
Abstract The cell nucleus is a dynamic structure repeating disassembly and reformation during mitosis. Reformation of the essential for proliferation, therefore, factors required nuclear are fundamental eukaryotes. Although various have been identified in vitro systems using frog egg extracts vivo imaging somatic cells, little known about formation functional structures living mouse eggs. To identify such factors, we used reconstruction approach to construct an artificial around DNA T4 phage...
Abstract In eukaryotes, DNA is housed within the cell nucleus. Molecules required for formation of a nucleus have been identified using in vitro systems with frog egg extracts and vivo imaging somatic cells. However, little known about physicochemical factors conditions nuclear mouse oocytes. this study, reconstitution approach purified DNA, we aimed to determine factors, such as amount timing introduction, nuclei transport activity T4 phage (~166 kbp) was microinjected into...
Abstract In female eutherian mammal development, X-chromosome inactivation (XCI) of one the two X chromosomes is initiated early. Understanding relationship between initiation XCI and cell fate critical for understanding early development requires a system that can monitor in single living cells. Traditional embryonic stem cells (ESCs) used studies often lose spontaneously during culture differentiation, making accurate monitoring difficult. Additionally, most assessment methods necessitate...
Abstract Histone H3 lysine36 dimethylation (H3K36me2) is generally distributed in the gene body and euchromatic intergenic regions. However, we found that H3K36me2 enriched pericentromeric heterochromatin some mouse cell lines. We here revealed mechanism of targeting H3K36me2. Among several H3K36 methyltransferases, NSD2 was responsible for inducing heterochromatic Depletion overexpression analyses NSD2-associating proteins recruitment to mediated through imitation switch (ISWI) chromatin...