A5081456364- Microtubule and mitosis dynamics
- 3D Printing in Biomedical Research
- Neurogenesis and neuroplasticity mechanisms
- Cellular Mechanics and Interactions
- Pluripotent Stem Cells Research
- Renal and related cancers
- Cancer-related gene regulation
- Hippo pathway signaling and YAP/TAZ
- Neuroscience and Neural Engineering
- Wnt/β-catenin signaling in development and cancer
University College London
2021-2024
Institute of Child Health
2022
University of Cambridge
2022
Wellcome/MRC Cambridge Stem Cell Institute
2022
Medical Research Council
2022
Morphogenesis requires embryonic cells to generate forces and perform mechanical work shape their tissues. Incorrect functioning of these force fields can lead congenital malformations. Understanding dynamic processes the quantification profiling three-dimensional mechanics during evolving vertebrate morphogenesis. Here we describe elastic spring-like sensors with micrometre-level resolution, fabricated by intravital bioprinting directly in closing neural tubes growing chicken embryos....
Post-zygotic mutations that generate tissue mosaicism are increasingly associated with severe congenital defects, including those arising from failed neural tube closure. Here we report fold elevation during mouse spinal neurulation is vulnerable to deletion of the VANGL planar cell polarity protein 2 (Vangl2) gene in as few 16% neuroepithelial cells. Vangl2-deleted cells typically dispersed throughout neuroepithelium, and each non-autonomously prevents apical constriction by an average five...
The early specification and rapid growth of extraembryonic membranes are distinctive hallmarks primate embryogenesis. These complex tasks resolved through an intricate combination signals controlling the induction lineages and, at same time, safeguarding pluripotent epiblast. Here, we delineate orchestrating epiblast amnion identity. We encapsulated marmoset stem cells into agarose microgels identified culture conditions for development epiblast- amnion-spheroids. Spatial identity mapping...
Neuroepithelial cells balance tissue growth requirement with the morphogenetic imperative of closing neural tube. They apically constrict to generate mechanical forces which elevate folds, but are thought dilate during mitosis. However, we previously reported that mitotic neuroepithelial in mouse posterior neuropore have smaller apical surfaces than non-mitotic cells. Here, document progressive enrichment non-muscle myosin-II mitotic, not non-mitotic, areas. Live-imaging chick confirms...
Abstract Neuroepithelial cells balance tissue growth requirement with the morphogenetic imperative of closing neural tube. They apically constrict to generate mechanical forces which elevate folds, but are thought dilate during mitosis. However, we previously reported that mitotic neuroepithelial in mouse posterior neuropore have smaller apical surfaces than non-mitotic cells. Here, document progressive enrichment non-muscle myosin-II mitotic, not non-mitotic, areas. Live-imaging chick...