A5055440929- Pluripotent Stem Cells Research
- 3D Printing in Biomedical Research
- CRISPR and Genetic Engineering
- Renal and related cancers
- Tissue Engineering and Regenerative Medicine
- Mesenchymal stem cell research
- Reproductive System and Pregnancy
- Pregnancy and preeclampsia studies
- Prenatal Screening and Diagnostics
- Organ and Tissue Transplantation Research
Wellcome/MRC Cambridge Stem Cell Institute
2021-2024
Medical Research Council
2021-2024
University of Cambridge
2021-2024
Human periimplantation development requires the transformation of naive pluripotent epiblast into a polarized epithelium. Lumenogenesis plays critical role in this process, as undergoes rosette formation and lumen expansion to form amniotic cavity. Here, we present high-throughput vitro model for morphogenesis. We established microfluidic workflow encapsulate human stem cells (hPSCs) monodisperse agarose microgels. Strikingly, hPSCs self-organized spheroids that could be maintained...
The early specification and rapid growth of extraembryonic membranes are distinctive hallmarks primate embryogenesis. These complex tasks resolved through an intricate combination signals controlling the induction lineages and, at same time, safeguarding pluripotent epiblast. Here, we delineate orchestrating epiblast amnion identity. We encapsulated marmoset stem cells into agarose microgels identified culture conditions for development epiblast- amnion-spheroids. Spatial identity mapping...
Abstract Biomechanical cues are instrumental in guiding embryonic development and cell differentiation. Understanding how these physical stimuli translate into transcriptional programs will provide insight mechanisms underlying mammalian pre-implantation development. Here, we explore this type of regulation by exerting microenvironmental control over mouse stem cells. Microfluidic encapsulation cells agarose microgels stabilizes the naive pluripotency network specifically induces expression...