A5060076025- Lysosomal Storage Disorders Research
- Metabolism and Genetic Disorders
- Alcoholism and Thiamine Deficiency
- Ion Transport and Channel Regulation
- Calcium signaling and nucleotide metabolism
- Aortic Disease and Treatment Approaches
- Trypanosoma species research and implications
- Cellular transport and secretion
- Caveolin-1 and cellular processes
- Connective tissue disorders research
- RNA modifications and cancer
- Studies on Chitinases and Chitosanases
- Biochemical and Molecular Research
- Aortic aneurysm repair treatments
General University Hospital in Prague
2013-2018
Charles University
2012-2018
A series of six full-term placentas and umbilical cords were examined using the in situ detection globotriaosylceramide (Gb3Cer), GM1 ganglioside (GM1), GM3 (GM3), cholesterol caveolin 1. Immunohistochemical study showed uniform distinct staining apical membrane villous capillary endothelial cells for Gb3Cer, GM1, cholesterol. There was also a strong signal The immunophenotype suggests presence caveola-associated raft microdomains. almost completely shared with extravillous intravascular...
We present a simple method for enrichment of lysosomal membranes from HEK293 and HeLa cell lines taking advantage selective disruption lysosomes by methionine methyl ester. Organelle concentrate postnuclear supernatant was treated with 20 mmol/l ester 45 min to lyse the lysosomes. Subsequently, were resolved on step sucrose gradient. An enriched membrane fraction collected 20%/35% interface. The washed 30 times relative homogenate gave yield more than 8 %. These results are comparable...