A5065408595- Gut microbiota and health
- Bacterial Identification and Susceptibility Testing
- Antibiotic Resistance in Bacteria
- Clostridium difficile and Clostridium perfringens research
- Cystic Fibrosis Research Advances
- Genomics and Phylogenetic Studies
- Urinary Tract Infections Management
- Bacterial biofilms and quorum sensing
- Head and Neck Cancer Studies
- Gastric Cancer Management and Outcomes
- Photodynamic Therapy Research Studies
- Probiotics and Fermented Foods
- Colorectal Cancer Treatments and Studies
- Nanoplatforms for cancer theranostics
- Cultural and Historical Studies
- Cancer Immunotherapy and Biomarkers
- Nosocomial Infections in ICU
- Pharmaceutical and Antibiotic Environmental Impacts
- Ocular Infections and Treatments
- Epigenetics and DNA Methylation
University of Zurich
2024-2025
University of Toronto
2018-2024
University Health Network
2018-2024
Toronto General Hospital
2021-2024
Canada Research Chairs
2024
Toronto General Hospital Research Institute
2021-2022
Classical preoperative skin antisepsis is insufficient in completely eliminating bacterial colonization for arthroplasty. In contrast, photodynamic therapy (PDT) with red light and methyl-aminolevulinate (MAL), combined antisepsis, led to the absence of growth healthy participants, though local erythema, posing an obstacle orthopedic surgery. Therefore, we explored whether artificial daylight PDT (PDT-DL) was superior light. Twenty participants were allocated either 5-aminolevulinic...
Abstract Background Determining relatedness of bacterial pathogens within hospitals is important to rule out transmission events, but current approaches are typically slow and/or resource intensive. We sought assess the utility rapid long-read sequencing with nearest neighbour-typing for identifying direct from specimens. Figure 1. Plot true and predicted genetic distances E. coli. (a) All comparisons. (B) Only concordant (C) comparisons LS<0.5. (D) LS>0.5. Green data points...
Discontinuation of inappropriate antimicrobial therapy is an important target for stewardship intervention. The drug and duration-dependent effects antibiotics on the developing neonatal gut microbiota needs to be precisely quantified.
Short-read sequencing can provide detection of multiple genomic determinants antimicrobial resistance from single bacterial genomes and metagenomic samples. Despite its increasing application in human, animal, environmental microbiology, including human clinical trials, the performance short-read Illumina for gene (ARG) detection, resistance-conferring nucleotide polymorphisms (SNPs), has not been systematically characterized. Using paired-end 2 × 150 bp (base pair) an assembly-based method...
Abstract We previously demonstrated that P. aeruginosa isolates persisted in children with cystic fibrosis (CF) despite inhaled tobramycin treatment had increased anti-Psl antibody binding vitro compared to those successfully eradicated. aimed validate these findings by directly visualizing CF sputum. This was a prospective observational study of new-onset infection who underwent eradication treatment. Using microbial identification passive clarity technique (MiPACT), visualized sputum...
ABSTRACT Intestinal colonization with pathogens and antimicrobial-resistant organisms (AROs) is associated increased risk of infection. Fecal microbiota transplant (FMT) has successfully been used to cure recurrent Clostridioides difficile infection (rCDI) decolonize intestinal AROs. However, FMT significant practical barriers safe broad implementation. Microbial consortia represent a novel strategy for ARO pathogen decolonization, safety advantages over FMT. We undertook an...
Abstract During a bacterial infection or colonization, the detection of antimicrobial resistance (AMR) is critical, but slow due to culture-based approaches for clinical and screening samples. Culture-based phenotypic AMR confirmation require up 72 hours (h) even weeks slow-growing bacteria. Direct shotgun metagenomics by long-read sequencing using Oxford Nanopore Technologies (ONT) may reduce time species gene identification. However, swabs complex range Gram-negative -positive bacteria,...
ABSTRACT Timely diagnostic tools are needed to improve antibiotic treatment. Pairing metagenomic sequencing with genomic neighbor typing algorithms may support rapid clinically actionable results. We created resistance-associated sequence elements (RASE) databases for Escherichia coli and Klebsiella spp . used them predict susceptibility in directly sequenced (Oxford Nanopore) urine specimens from critically ill patients. RASE analysis was performed on pathogen-specific reads sequencing....
2515 Background: The gut microbiome modifies response to ICI treatment. Despite durable rates seen with ICI, most pts do not benefit from treatment (primary resistance) or have only a period of disease control (acquired resistance). Immune Resistance Interrogation Study (IRIS, NCT03702309) is prospective pan-cancer study that aims at characterizing ICI-resistant through multiomic and microbial composition profiling. Herein we compare the diversity PR vs AR PD-1/PD-L1 based as part wider IRIS...
The development of the infant gut microbiota influences maturation host immune system and has been implicated in both short- long-term health outcomes. In a healthy infant, initial stages are characterized by progressive acquisition proliferation anaerobes. neonatal intensive care unit (NICU), infants often exposed to antibiotics which disturb normal microbiota. this retrospective cross-sectional study, we aimed evaluate effects three different antibiotic regimens on NICU, focusing effect...
Abstract Intestinal colonisation with pathogens and antimicrobial resistant organisms (AROs) is associated increased risk of infection. Fecal microbiota transplant (FMT) has successfully been used to cure recurrent Clostridioides difficile infection (rCDI) decolonise intestinal AROs. However, FMT significant practical barriers implementation. A microbial consortium, ecosystem therapeutic (MET)-2, an alternative for the treatment rCDI. It unknown whether MET-2 decreases in resistance genes...
Abstract Short-read sequencing provides a culture-independent method for the detection of antimicrobial resistance (AMR) genes from single bacterial genomes and metagenomic samples. However, performance characteristics these approaches have not been systematically characterized. We compared assembly- read-based to determine sensitivity, positive predictive value, limits required AMR gene in an Escherichia coli ST38 isolate spiked into synthetic microbial community at varying abundances....