A5015412173- Pulmonary Hypertension Research and Treatments
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Dermatologic Treatments and Research
- COVID-19 Clinical Research Studies
- Glaucoma and retinal disorders
- Wnt/β-catenin signaling in development and cancer
- Mast cells and histamine
- Monoclonal and Polyclonal Antibodies Research
- Drug-Induced Adverse Reactions
- Galectins and Cancer Biology
- Glycosylation and Glycoproteins Research
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Neurological Disorders and Treatments
- Respiratory Support and Mechanisms
- Inflammasome and immune disorders
- Skin and Cellular Biology Research
- Contact Dermatitis and Allergies
- Asthma and respiratory diseases
- Phosphodiesterase function and regulation
- Connective Tissue Growth Factor Research
Universitat de València
2017-2024
Inflammasome activation is one of the first steps in initiating innate immune responses. In this work, we studied inflammasomes airways critically ill COVID-19 patients and effects N-acetylcysteine (NAC) on inflammasomes. Tracheal biopsies were obtained from without no respiratory disease (control, n = 32), SARS-CoV-2 B.1 variant (n 31), B.1.1.7 VOC alpha 20) patients. Gene expression protein measured by RT-qPCR immunohistochemistry. Macrophages bronchial epithelial cells stimulated with...
There is a pressing medical need for improved treatments in skin fibrosis including keloids and hypertrophic scars (HTS). This study aimed to characterize the role of phosphodiesterase 4 (PDE4), specifically PDE4B fibrotic remodeling vitro vivo. In vitro, effects PDE4A-D (Roflumilast) or (siRNA) inhibition on TGFβ1-induced myofibroblast differentiation dedifferentiation were studied normal (NHDF) keloid (KF) human dermal fibroblasts. vivo, PDE4 HOCl-induced mice was addressed preventive...
Lung inflammation in COPD is poorly controlled by inhaled corticosteroids (ICS). Strategies to improve ICS efficacy or the search of biomarkers who may select those patients candidates receive are needed. Recent data indicate that MUC1 cytoplasmic tail (CT) membrane mucin can mediate corticosteroid chronic rhinosinusitis. The objective this work was analyze previously unexplored role on vitro and vivo models. MUC1-CT expression measured real time PCR, western blot, immunohistochemistry...
Abstract Background Epidermal remodeling and hypertrophy are hallmarks of skin fibrotic disorders, keratinocyte to mesenchymal (EMT)-like transformations drive epidermis alteration in fibrosis such as keloids hypertrophic scars (HTS). While phosphodiesterase 4 (PDE4) inhibitors have shown effectiveness various their role is not fully understood. This study aimed explore the specific PDE4B epidermal seen fibrosis. Methods In vitro experiments examined effects inhibiting PDE4A-D (with...
Delayed-type hypersensitivity (DTH) is caused by a broad number of drugs used in clinic, and antineoplastic show an elevated proportion DTH, which potentially affects the quality life patients. Despite serious problem negative economic impact deriving from market withdrawal such high hospitalization costs, nowadays, there are no standard validated methods vitro or vivo to evaluate sensitizing potential preclinical phase. Enhanced predictions safety evaluations really important, for that...
Skin fibrosis is a hallmark of wide array dermatological diseases which can greatly impact the patients' quality life. Galectin-3 (GAL-3) has emerged as central regulator tissue fibrosis, playing an important pro-fibrotic role in numerous organs. Various studies are highlighting its importance skin fibrotic biomarker; however, there need for further that clarify role. This paper aims to ascertain whether expression GAL-3 increased relevant vitro and vivo models fibrosis. We studied using...
<b>Background:</b> Pulmonary hypertension (PH) in idiopathic pulmonary fibrosis (IPF) portends a poor prognosis and currently there is no approved therapy for hypoxemic PH. IL-11 novel lung mediator but its role on vascular function unknown. <b>Objective:</b> To analyze the of artery remodeling vitro vivo. <b>Methods:</b> expression was evaluated arteries sections control subjects patients with IPF, IPF+PH PH (PAH). Human endothelial cells smooth muscle were stimulated IL-11. Endothelial to...
<b>Background:</b> Idiopathic pulmonary fibrosis (IPF) is characterized by a rapid progressive lung decline and premature death after its diagnosis. Roflumilast displayed anti-fibrotic effects in animal cellular models. Recent studies indicate that the combination of PDE4 PDE5 inhibitors (sildenafil) potentiates properties each drug, suggesting potential beneficts this combination. <b>Objectives:</b> To study from adding sildenafil to roflumilast N-oxide (RNO) inhibiting TGFβ1-induced human...
<b>Background:</b> Chronic Obstructive Pulmonary Disease (COPD) is an inflammatory lung disease caused by chronic exposure to cigarette smoke. Oxidative stress one of the most important mechanisms involved in physiopathology COPD. Nuclear Factor Erythroid 2-related (Nrf2) critical protection against oxidative inducing expression antioxidant genes and a decrease its has been observed COPD patients. <b>Objective:</b> The aim this study was characterise effects Bardoxolone, Omavexolone...
<b>Background:</b> Chronic Obstructive Pulmonary Disease (COPD) is a progressive inflammatory lung disease characterized by an accumulation of circulating leukocytes. The phsosphodiestare4 (PDE4) inhibitor roflumilast has been approved for the treatment severe COPD as add on therapy to bronchodilators and inhaled corticosteroids. However, side effects including gastrointestinal limit clinical use. <b>Objective:</b> Demonstrate efficacy GSK256066, selective PDE4B leukocyte-endothelial cell...
<b>Background:</b> Chronic Obstructive Pulmonary Disease (COPD) is an irreversible progressive inflammatory lung disease. Cigarette smoking the principal cause in this condition. Inflammation and oxidative stress are important features pathogenesis of Nuclear Factor Erythroid 2-related (Nrf2) critical cytoprotection by inducing expression antioxidant enzymes. <b>Methods:</b> Peripheral blood neutrophils primary human bronchial cells (HBEC) from COPD healthy volunteers, as well as, monocytes...