A5012347027- Allergic Rhinitis and Sensitization
- Dermatology and Skin Diseases
- Asthma and respiratory diseases
- Food Allergy and Anaphylaxis Research
- Biofuel production and bioconversion
- Antimicrobial Resistance in Staphylococcus
- Pharmaceutical and Antibiotic Environmental Impacts
- Neonatal Respiratory Health Research
- Enzyme Catalysis and Immobilization
- Botulinum Toxin and Related Neurological Disorders
- Bacterial biofilms and quorum sensing
- Microbial Metabolic Engineering and Bioproduction
- Bacteriophages and microbial interactions
- Analytical chemistry methods development
- Cerebral Palsy and Movement Disorders
- Enzyme-mediated dye degradation
- Mycobacterium research and diagnosis
- Ocular Infections and Treatments
- Ophthalmology and Visual Health Research
- Advanced oxidation water treatment
- S100 Proteins and Annexins
- Catalysis for Biomass Conversion
- CO2 Sequestration and Geologic Interactions
- Membrane-based Ion Separation Techniques
- Wastewater Treatment and Reuse
Alberta Health Services
2018-2024
University of Calgary
2018-2024
Eulji University
2019-2023
Calgary Laboratory Services
2018
Daegu University
2017
S100A8 and S100A9 are important proteins in the pathogenesis of allergy.Asthma is an allergic lung disease, characterized by bronchial inflammation due to leukocytes, bronchoconstriction, allergen-specific IgE.In this study, we examined role interaction cytokine release from epithelial cells, with constitutive apoptosis neutrophils.S100A8 induce increased secretion neutrophil survival cytokines such as MCP-1, IL-6 IL-8.This suppressed TLR4 inhibitor), LY294002, AKT inhibitor, PD98059,...
An increasing number of severe infections caused by Staphylococcus aureus ST398 strains has been observed. However, it not elucidated whether all are equally virulent. We collected 13 from China and Canada to test in a Caenorhabditis elegans infection model compared their whole genome sequences (WGS) explore potential insights into virulence. All isolates belonged ST398-methicillin-susceptible S. (MSSA) with variant spa types (t034, t571, t1451, t1250). Pulsed field gel electrophoresis...
S100A8 and S100A9 function as essential factors in inflammation, also exert anti-tumor or tumorigenic activity depending on the type of cancer. Chronic eosinophilic leukemia (CEL) is a rare hematological malignancy having elevated levels eosinophils, characterized by presence FIP1L1-PDGFRA fusion gene. In this study, we examined pro-apoptotic mechanisms FIP1L1-PDGFRα+ cells hypereosinophilic patient cells. induce apoptosis EoL-1 via TLR4. The surface TLR4 expression increased after exposure...
Staphylococcus aureus sequence type (ST) 398 is a lineage affecting both humans and livestock worldwide. However, the mechanisms underlying its clonal evolution are still not clearly elucidated. We applied whole-genome sequencing (WGS) typing to 45 S. strains from China Canada between 2005 2014, in order gain insight into their evolutionary pathway. Based on WGS phylogenetic analysis, 42 isolates were assigned human-associated clade (I/II-GOI) 3 livestock-associated (IIa). Phylogeny of ϕSa3...
It is difficult to treat allergic diseases including asthma completely because its pathogenesis remains unclear. House dust mite (HDM) a critical allergen and Toll-like receptor (TLR) 4 member of the toll-like family, which plays an important role in diseases. The purpose this study was characterize novel allergen, Der f 38 binding TLR4, unveil as inducer allergy. expression detected body feces Dermatophagoides farinae (DF). Electron microscopy revealed that it located granule layer,...
Atopic dermatitis (AD) is a chronic relapsing pruritic disease encompassing skin inflammation and barrier dysfunction. House dust mites are key allergens that augment the development of atopic dermatitis. We aimed to investigate pathogenic mechanism AD due Der p 38, recently identified by us. The frequency IgE reactivity 38 in subjects was 52.6% (10/19) prick test 57.9% (11/19) dot blot assay. In human keratinocyte HaCaT cells, triggered impairment filaggrin expression induced...
The aromatic amino acids, Tyr or Trp, which line the active-site walls of esterases, stabilize catalytic His loop via hydrogen bonding. A residue is preferred in extremophilic esterases (psychrophilic hyperthermophilic esterases), whereas a Trp moderate-temperature esterases. Here, we provide evidence that and play distinct roles cold adaptation psychrophilic esterase EstSP1 isolated from an Arctic bacterium Sphingomonas glacialis PAMC 26605. Stern-Volmer plots showed mutation Tyr191 to Ala,...
The genomic comparison of virulent (TW20), moderately (CMRSA6/CMRSA3) and avirulent (M92) strains from a genetically closely-related MRSA ST239 sub-lineage revealed striking similarities in their genomes antibiotic resistance profiles, despite differences virulence pathogenicity. main were the spa gene (coding for staphylococcal protein A), lpl genes lipoprotein-like membrane proteins), cta (genes involved heme synthesis), dfrG trimethoprim-resistant dihydrofolate reductase), as well...
This study investigated the anti-allergic effect of Poncirus trifoliata (L.) Raf.(PT) on human keratinocytic HaCaT cells in vitro and 2,4-dinitrochlorobenzene (DNCB)-induced atopic dermatitis-like lesions vivo.The release TARC, MCP-1, IL-6 IL-8 is increased by IFN-γ TNF-α cells, PT extract suppressed production IL-6, IL-8.PT recovered expression filaggrin decreased TNF-α. vivo experiment, administration skin severity score, thickening epidermis, movement inflammatory into dermis, serum IgE...
Abstract The house dust mite is the most common cause of allergic diseases, and TLR4 acts as an overarching receptor for responses. This study aimed to identify novel allergen binding in mites unveil its unique role Der p 38 was purified characterized by liquid chromatography tandem mass spectrometry–based peptide mapping. Biolayer interferometry structure modeling unveiled TLR4-binding activity recombinant 38. allergenicity confirmed a skin prick test, basophil activation dot blot assays....
Spasticity is a common issue among children, especially those with bilateral spastic cerebral palsy (CP). Selective dorsal rhizotomy (SDR) surgical procedure that often used to decrease lower limb rigidity, alongside other treatment options such as intrathecal medication, peripheral nerve surgery, and deep brain stimulation (DBS). The objective of these therapies improve the standard living for young individuals. This article intends explain motor deficits observed in diplegia rehabilitation...
Chaenomeles sinensis Koehne (CS) has been used in a traditional oriental medicine for treating throat diseases, anaphylaxis, viral infection, and inflammation.This study investigated the underlying mechanism of anti-allergic effect CS.Leaves CS plants were dried, powdered, then underwent extraction with DMSO.Both ELISA western blotting performed to evaluate cytokine concentration expression activation filaggrin JNK.Five-week-old female NC/Nga mice as an AD-like mouse model by them...
Purpose: To assess the pattern of common eye diseases in children visitingtertiary hospital Karachi.Material and Methods: A random cross sectional survey was conducted duringtwo weeks data recorded through a performa containing details biodata, personal history, presenting complains, aided un visual acuity,refractive error signs adnexa ocular alignment. Data wasanalyzed using SPSS 16.0.Results: total 250 patients were examined out which male were52.8% females constitutes 47.2%. Majority...
The DNA Comet assay (microgel electrophoresis method) is a recommended technique for detection of radiation treatment in food containing material. It involves isolating cells/nuclei (DNAs) from both un-irradiated and irradiated samples embedding them on microscope slides, lysing the cells/nuclei, performing electrophoresis, staining then evaluating patterns migration agarose gel. In this study, was applied to identify grains seeds commonly consumed by humans animals such as wheat, melon...
Background: The house dust mite (HDM) is the most common cause of allergic diseases, and TLR4 acts as an overarching receptor for responses. This study aimed to identify novel allergen binding in HDMs unveil its unique role Methods: Der p 38 was purified characterized by LC-MS/MS-based peptide mapping. Biolayer interferometry structure modeling unveiled TLR4-binding activity recombinant 38. allergenicity confirmed a skin prick test, basophil activation dot blot assays. Results: test...