Endothelial nitric-oxide synthase (eNOS) is phosphorylated at Ser-1179 (bovine sequence) by Akt after growth factor or shear stress stimulation of endothelial cells, resulting in increased eNOS activity. Purified also Thr-497 purified AMP-activated protein kinase, decreased We investigated whether bradykinin (BK) bovine aortic cells (BAECs) regulates through activation and phosphorylation. transiently activated BK-stimulated BAECs. Activation blocked completely wortmannin LY294002,...

PURPOSE Using the technique of recursive partitioning and amalgamation analysis with verification, Pediatric Oncology Group (POG) investigated independent prognostic significance previously published factors significantly associated event-free survival (EFS) in B-progenitor cell acute lymphoblastic leukemia (ALL). PATIENTS AND METHODS Age, leukocyte count, sex, immunophenotype (expression cytoplasmic immunoglobulin [Ig] surface antigens CD10 CD34), DNA index (ratio flow cytometry-determined...

Endothelial nitric-oxide synthase (eNOS) is regulated by signaling pathways involving multiple sites of phosphorylation. The coordinated phosphorylation eNOS at Ser¹¹⁷⁹ and dephosphorylation Thr⁴⁹⁷activates the enzyme, whereas inhibition results when Thr⁴⁹⁷ phosphorylated dephosphorylated. We have identified two further sites, Ser⁶¹⁷ Ser⁶³⁵, phosphopeptide mapping matrix-assisted laser desorption ionization time flight mass spectrometry....

Objective and Method Findings from both animal human research suggest that pain sensitivity changes across the menstrual cycle; however, among humans nature of these cycle effects remains unclear. The present study used a repeated-measures design to evaluate in thermal ischemic responses during three phases cycle, midfollicular (postmenstrual), ovulatory, mid-to-late luteal (premenstrual), 11 healthy women. phase which subjects began their participation was determined randomly. Plasma levels...

Objective Patients experiencing temporomandibular disorders (TMD) show greater sensitivity to painful stimuli than age- and gender-matched control subjects. This enhanced pain may result, at least in part, from an alteration regulatory systems that are influenced by resting arterial blood pressure. In this study, we examined the relationship between systolic pressure perception 64 female TMD 23 age-matched pain-free Method Resting measures of thermal ischemic threshold tolerance were...

PURPOSE The Pediatric Oncology Group (POG) acute leukemia in childhood (ALinC) 13 study tested two treatment regimens that used different CNS chemoprophylaxis for children older than 12 months with non-T, non-B lymphoblastic (ALL) and no demonstrable disease at diagnosis. PATIENTS AND METHODS With the first regimen, standard (S), six injections of triple intrathecal chemotherapy (TIC), consisting methotrexate (MTX), hydrocortisone (HC), cytarabine (ara-C), were administered during...

The aim of this study was to determine the effects acute bouts exercise on myocardial recovery after ischemia and heat shock protein expression. Adult female Sprague-Dawley rats were divided into five groups: 1) 1-day run (1DR; n = 6) 2) 3-day (3DR; 7), in which ran for 100 min at a speed 20 m/min up 6° grade 1 or 3 consecutive days; 3) cold (1CR), same as 1DR but with wet fur 8°C, prevented an elevation core temperature ( 8); 4) sedentary (HS), had their temperatures raised 42°C one time 15...

This study determined the role of body temperature during chronic exercise on myocardial stress proteins and antioxidant enzymes as well functional recovery after an ischemic insult. Male Sprague-Dawley rats were exercised for 3, 6, or 9 wk in a 23°C room (3WK, 6WK, 9WK, respectively) 4–8°C environment with wetted fur (3WKC, 6WKC, 9WKC, respectively). The colder prevented elevations core temperature. During weeks 3–9 animals ran 5 days/wk up 6% grade at 20 m/min 60 min. Myocardial heat shock...

3-Hydroxy-3-methylglutaryl (HMG)-CoA reductase inhibitors, statins, provide beneficial effects independent of their lipid-lowering effects. One effect appears to involve acute activation endothelial nitric oxide (NO) synthase (eNOS) and increased NO release. However, the mechanism statin-stimulated eNOS is unknown. Therefore, we hypothesized that may be coupled altered phosphorylation. Bovine aortic cells (BAECs), passages 2-6, were treated with either lovastatin or pravastatin from 0 30...

Soluble guanylate cyclase (sGC) is an important downstream intracellular target of nitric oxide (NO) that produced by endothelial NO synthase (eNOS) and inducible (iNOS). In this study, we demonstrate sGC exists in a complex with eNOS heat shock protein 90 (HSP90) aortic cells. addition, show smooth muscle cells, forms HSP90. Formation the sGC/eNOS/HSP90 increased response to eNOS-activating agonists manner depends on HSP90 activity. vitro binding assays glutathione S-transferase fusion...

Objective: We have previously reported that patients with temporomandibular disorders (TMD) exhibit enhanced sensitivity to experimentally evoked pain (1); however, the clinical relevance of this increased remains unclear. The purpose study was investigate relationship experimental and psychosocial variables among TMD. Design: Thirty-six TMD were studied, half whom sensitive (PS) other tolerant (PT), based on their ability tolerate an ischemic task. Responses painful thermal nonpainful...

Tseng, C-Y, Lee, J-P, Tsai, Y-S, S-D, Kao, C-L, Liu, T-C, Lai, C-S, Harris, MB, and Kuo, C-H. Topical Cooling (Icing) Delays Recovery From Eccentric Exercise–Induced Muscle Damage. J Strength Cond Res 27(5): 1354–1361, 2013—It is generally thought that topical cooling can interfere with blood perfusion may have positive effects on recovery from a traumatic challenge. This study examined the influence of muscle damage markers hemodynamic changes during eccentric exercise. Eleven male subjects...

Amyloid β (Aβ)-induced vascular dysfunction significantly contributes to the pathogenesis of Alzheimer's disease (AD). Aβ is known impair endothelial nitric oxide synthase (eNOS) activity, thus inhibiting production (NO).In this study, we investigated Aβ-effects on heat shock protein 90 (HSP90) interaction with eNOS and Akt in cultured cells also explored role oxidative stress process.Treatments (EC) promoted constitutive association HSP90 but abrogated agonist (vascular growth factor...

Bradykinin (BK) is an important physiological regulator of endothelial cell function. In the present study, we have examined role Janus-activated kinase (JAK)/signal transducers and activators transcription (STAT) pathway in signal transduction through BK B2 receptor (B2R). cultured bovine aortic cells (BAECs), activates Tyk2 JAK family tyrosine kinases. Activation results phosphorylation subsequent nuclear translocation STAT3. also mitogen-activated p44 p42 protein kinases, resulting STAT3...

Endothelial nitric oxide (NO) synthase (eNOS) is regulated by heat shock protein 90 (HSP90), a heat-inducible protein; however, the effect of on eNOS expression and eNO release unknown. Bovine aortic endothelial cells were incubated for 1 h at 37°C, 42°C, or 45°C cell lysates evaluated with use Western blotting. We observed 2.1 ± 0.1-fold increase in content, but no change HSP90 HSP70 HSP90/eNOS association, 24 after 42°C. also 7.7 1.5-fold did not observe 45°C. activity maximal...

To examine the effects of aging on neuromuscular adaptations to resistance training (i.e., weight lifting), young (9 months age) and aged (20 male rats either participated in a 7‐week ladder climbing protocol with additional attached their tails or served as controls (n = 10/group). At conclusion, were euthanized hindlimb muscles quickly removed frozen for later analysis. Longitudinal sections soleus plantaris collected, pre‐ postsynaptic features junctions (NMJs) visualized...

Bradykinin (BK) is an important physiological regulator of endothelial cell function. In the present study, we have examined role Janus-activated kinase (JAK)/signal transducers and activators transcription (STAT) pathway in signal transduction through BK B2 receptor (B2R). cultured bovine aortic cells (BAECs), activates Tyk2 JAK family tyrosine kinases. Activation results phosphorylation subsequent nuclear translocation STAT3. also mitogen-activated p44 p42 protein kinases, resulting STAT3...

The combination of ifosfamide (I) and etoposide (E) was useful in salvaging patients with recurrent/resistant malignant solid tumors childhood. Carboplatin (C), active against a number pediatric cancers, added to I E form three-drug called ICE improve the response rate.ICE, consisting 1.5 g/m2 plus 100 mg/m2 i.v.q.d. x 3 C i.v. on day only, given 21-28-day intervals. started at 300 mg/m2, dose escalated 25% increments, three evaluable treated each level.Ninety-two were enrolled this phase...

Journal Article Growing Old Gracefully: Age Concealment and Gender Get access Mary B. Harris University of New Mexico Search for other works by this author on: Oxford Academic PubMed Google Scholar Gerontology, Volume 49, Issue 4, July 1994, Pages P149–P158, https://doi.org/10.1093/geronj/49.4.P149 Published: 01 1994 history Received: 15 March 1993 Accepted: 07 September

eNOS (endothelial nitric oxide synthase) catalyses the conversion of L-arginine into L-citrulline and NO. Evidence has been presented previously that is associated with CAT (cationic amino acid transporter)-1 arginine transporter in endothelial caveolae, it proposed eNOS–CAT-1 association facilitates delivery extracellular to eNOS. Definitive proof a protein–protein interaction between CAT-1 lacking, however, also unknown whether two proteins interact directly or via an adaptor protein. In...

PURPOSE To compare efficacy and toxicity of two schedules intermediate-dose methotrexate (IDM) cytarabine (Ara-C) in remission consolidation childhood acute lymphoblastic leukemia (ALL). PATIENTS AND METHODS In 1986, the Pediatric Oncology Group (POG) began a randomized trial to test chemotherapy children with newly diagnosed B-precursor cell ALL. MTX Ara-C were given as overlapping 24-hour infusions. The dose sequence administration based on preclinical model that had demonstrated synergism...