A5043476732- Advanced Proteomics Techniques and Applications
- Microfluidic and Capillary Electrophoresis Applications
- Scientific Computing and Data Management
- Bacteriophages and microbial interactions
- Semantic Web and Ontologies
- Spaceflight effects on biology
- Research Data Management Practices
- Biomedical Text Mining and Ontologies
- Molecular Biology Techniques and Applications
- Protein purification and stability
- Spectroscopy Techniques in Biomedical and Chemical Research
- Plant Virus Research Studies
- Mass Spectrometry Techniques and Applications
- Gene expression and cancer classification
- Cell Image Analysis Techniques
- Metabolomics and Mass Spectrometry Studies
- Proteins in Food Systems
- Microfluidic and Bio-sensing Technologies
- Biosensors and Analytical Detection
- DNA Repair Mechanisms
- Machine Learning in Bioinformatics
- Analytical Chemistry and Chromatography
- Bioinformatics and Genomic Networks
- Pharmaceutical Practices and Patient Outcomes
- Food composition and properties
Institute of Informatics of the Slovak Academy of Sciences
2015
Institute of Macromolecular Chemistry
2000
Eunice Kennedy Shriver National Institute of Child Health and Human Development
1987-1991
National Institutes of Health
1987-1991
Forschungs- und Beratungsstelle Arbeitswelt
1987
Austrian Competence Centre of Food Safety
1983
When monolayers of tissue cancer cells various origins are exposed to real or simulated microgravity, many leave the monolayer and assemble three-dimensional (3D) aggregates (spheroids). In order define cellular machinery leading this change in growth behavior FTC-133 human thyroid MCF-7 breast cells, we recently performed proteome analyses on these cell lines determined proteins’ accumulation grown under 1g-conditions as well spheroids assembled microgravity during three 14 days,...
Access to consistent, high-quality metadata is critical finding, understanding, and reusing scientific data. However, while there are many relevant vocabularies for the annotation of a dataset, none sufficiently captures all necessary metadata. This prevents uniform indexing querying dataset repositories. Towards providing practical guide producing high quality description biomedical datasets, W3C Semantic Web Health Care Life Sciences Interest Group (HCLSIG) identified Resource Description...
Abstract Negatively charged polystyrene latex particles with electron microscopically determined radii of 22.4, 26.9, 27.6, 43.5, 44.2 and 59.4 nm (nominal provided by the manufacturer 28.5 to 75 nm) were found be applicable as size standards for quantitative agarose gel electrophoresis viruses cellular in that range. In at pH 6.5, 0.03 M ionic strength presence 10 m 3‐[(3‐cholamidopropyl)‐dimethylammonio]‐1‐propane sulfonate (CHAPS), exhibit non‐linear, reproducibly convex‐sigmoidal...
The adhesion behavior of human tissue cells changes in vitro, when gravity forces affecting these are modified. To understand the mechanisms underlying changes, proteins involved cell-cell or cell-extracellular matrix adhesion, their expression, accumulation, localization, and posttranslational modification (PTM) regarding during exposure to microgravity were investigated. As sialylation is influencing cell on Earth vitro vivo, we analyzed molecules detected by omics studies cells, which...
Access to consistent, high-quality metadata is critical finding, understanding, and reusing scientific data. However, while there are many relevant vocabularies for the annotation of a dataset, none sufficiently captures all necessary metadata. This prevents uniform indexing querying dataset repositories. Towards providing practical guide producing high quality description biomedical datasets, W3C Semantic Web Health Care Life Sciences Interest Group (HCLSIG) identified Resource Description...
Abstract Two procedures of computer simulation the electrophoretic migration a particle through agarose gel are described which allow for: (a) characterization fiber dimensions as function concentration (gel standardization), (b) determination radius and dynamics apparent compressibility during passage standardized gel, (c) estimation net charge density by calculating its mobility at 0 % concentration. The common model underlying these simulations is based on extended Ogston theory...
Abstract A recent study [1] has demonstrated the feasibility of obtaining Ferguson plots in agarose gel electrophoresis, using a single pore gradient gel. We now report three remedies for defects previous experimental approach: (i) UV‐absorbing media density stabilization is avoided by replacing 5‐( N ‐2, 3‐dihydroxypropylacetamido)‐2, 4, 6‐triiodo‐ , ′bis (2, 3‐dihydroxypropyl) isophthalamide (Nycodenz) with heavy water; this renders method applicable to ethidium bromide‐labeled DNA. (ii)...
Abstract A program in BASIC suitable for personal computers is described which applicable to gel electrophoresis conducted a single (continuous) buffer. The curve fitting polynomial function, allowing an objective selection of the most appropriate type and order – linear, convex or concave particular application. Results do not differ significantly from previous programs evaluation linear Ferguson plots exponential function evaluating plots, executed on mainframe such as DEC‐10 (Digital) IBM...
Abstract This study presents a computerized evaluation of pore gradient gel electrophoretograms to arrive at estimates for both the particle‐free mobility and retardation coefficient, which is related particle size. Agarose gels ranging from 0.2 1.1% agarose were formed. Gel gradients stabilized during their formation by density 0–20% 5‐( N ‐2, 3‐dihydroxypropylacetamido)2, 4, 6‐triiodo‐ , ′ bis‐(2, 3‐dihydroxypropyl)‐isophthalamide (Nycodenz). Densitometry gelled‐in Bromophenol Blue showed...
Abstract Three plant viruses: turnip crinkle (TCV), hibiscus chlorotic ringspot (HCRSV) and pelargonium flowerbreak (PFBV), polystyrene size standards with radii of 22.4–59.4 nm can be stacked within trailing leading ion net mobilities 0.059 to 0.273 (relative Na + ). Stacking was carried out at pH 6.50, 0.03 M ionic strength, 50 mM 3–[(3‐cholamidopropyl)‐dimethylammonio]‐1‐propane sulfonate, a gel concentration 0.4 %, in agarose electrophoresis conducted 1.2 mA/cm 2 gel. Unstacking occurs...
All synthetic carrier ampholyte mixtures (SCAMs) contain some naturally fluorescing ampholytes (CAs). The detection of these during isoelectric focusing (IEF), using gel electrophoresis apparatus with intermittent scanning fluorescence, allows one to follow in real-time the "life cycle" pH gradient, i.e., its genesis, steady-state, and decay. most prominently CAs can be calibrated by measurement at or after steady state ("calibration CAs"). By application calibration CAs, fluorescence...
The extreme accuracy of spectrometrically determined masses proteins has opened the possibility to identify separated as gel electrophoretic bands in absence specific immunologic ways identification. For purpose protein transfer from mass spectrometer, electroelution intact advantages, particular when apparatus with capacity for fluorescent scanning allows one direct cell over band under computer control. To avoid labeling which is incompatible spectrometric identification, it proposed...
Access to consistent, high-quality metadata is critical finding, understanding, and reusing scientific data. However, while there are many relevant vocabularies for the annotation of a dataset, none sufficiently captures all necessary metadata. This prevents uniform indexing querying dataset repositories. Towards providing practical guide producing high quality description biomedical datasets, W3C Semantic Web Health Care Life Sciences Interest Group (HCLSIG) identified Resource Description...
Access to consistent, high-quality metadata is critical finding, understanding, and reusing scientific data. However, while there are many relevant vocabularies for the annotation of a dataset, none sufficiently captures all necessary metadata. This prevents uniform indexing querying dataset repositories. Towards providing practical guide producing high quality description biomedical datasets, W3C Semantic Web Health Care Life Sciences Interest Group (HCLSIG) identified Resource Description...
When culturing cells in space or under altered gravity conditions on Earth to investigate the impact of gravity, their adhesion and organoid formation capabilities change. In search a target where alteration force could have this impact, we investigated p130cas/BCAR1 its interactions more thoroughly, particularly as activity is sensitive applied forces. This protein well characterized regarding role growth stimulation processes. To better understand BCAR1′s force-dependent scaffolding other...