The aim of this study was to assess the cytotoxic effects various concentrations miltefosine on Leishmania major (MRHO/IR/75/ER) and L. tropica (MHOM/IR/02/Mash10) promastigotes observe programmed cell death features. colorimetric MTT assay used find viability obtained results were expressed as 50% inhibitory concentration (IC50). Also, effective doses (ED50) for amastigotes also determined. Annexin-V FLUOS staining performed properties using FACS analysis. Qualitative analysis total genomic...

Background: Blastocystis hominis is a common globally distributed parasite. The prevalence of this parasite has been shown to vary among different countries. Molecular studies have also that there high level genetic diversity spp. isolated from humans and animals. Extensive information on parasitic genotypes will aid in devising more effective strategies for the identification potential control these pathogenic parasites. Objectives: This study aimed gain abundance subtypes Iran. Materials...

Toxoplasma gondii is an obligate intracellular protozoan parasite causing toxoplasmosis in animals and humans. Primary maternal infection with during pregnancy frequently associated transplacental transmission to the fetus. However it not certain whether can cause recurrent abortion. The aim of this study was determine relationship between abortion via detection anti‐ antibodies sera women obstetrical problems compare results control group consisting history normal delivery. Sera from 130...

1. The aim of this work was to determine the frequency occurrence Toxoplasma gondii and genetically analyse isolates from a number avian hosts in southwest Iran (Khuzestan province). T. determined free-range chickens (Gallus domesticus), sparrows (Passer pigeons (Columba livia) starlings (Sturnus vulgaris). 2. Isolates obtained Toxoplasma-infected birds were subjected molecular typing by PCR-restriction fragment length polymorphism (RFLP) with sequence analysis GRA6 gene. 3. results showed...

Background: The aim of present study was describing a real-time PCR assay for the diagnosis and direct identification Leishmania species on Giemsa-stained slides in south-west Iran.

Intestinal parasitic infections (IPIs) are widely distributed throughout the world, especially in developing countries. Mortality and morbidity rates of these significant. Infections mainly transmitted via ingestion water, soil or food contaminated by faeces containing cysts ova protozoans helminthes, respectively. In many cases, clinical features remain asymptomatic consequently carriers act as on-going sources infection within their community. this regard, handlers a community may...

IntroductionLeishmaniasis, as a major health concern exists in 14 out of 22 countries the Eastern Mediterranean Region (EMR). Therefore, aim present investigation was to evaluate epidemiological features and spatial distribution cutaneous leishmaniasis (CL) during six consecutive years (2009–2014).Material methodsIn current retrospective cross-sectional study among 2009–2014, simple direct smear taken from all suspicious CL subjects who referred centers affiliated Ahvaz Jundishapur...

Abstract Hydatidosis is an important public health problem in several parts of Iran. The aim this molecular study to investigate Echinococcus granulosus genotypes as the causative agents hydatidosis south-west Iran (Khuzestan province). In study, isolates 334 hydatid cysts were collected from liver and lungs 141 sheep, 104 cattle, 84 goats 5 human cases. DNA was extracted examined by nested polymerase chain reaction (PCR) ribosomal (rDNA) internal transcribed spacer 1 (ITS1) restriction...

Infections due to protozoa of the genus Leishmania are responsible for a significant burden disease, especially in developing countries. Furthermore, incidence leishmaniasis continues rise lack vaccine. Drugs commonly used treatment disease show varying level effectiveness and also have associated side effects. There is therefore, need develop newer drug therapies. The aim our study was assess antiprotozoal activity ethanolic methanolic extracts leaf Satureja khuzestanica against major...

Uncooked vegetables are an important part of a healthy diet in different parts the world. The present study was carried out to determine parasitological contamination sold at markets Ahvaz southwest Iran. Parasitological examinations were performed material derived from 135 detect intestinal parasites. Each sample washed with physiological saline solution, allowing sedimentation room temperature for 24 h. Ten milliliter sediment centrifuged 3000 rpm 5 min. Sediment studied lugol stained...

Microsporidia are obligate intracellular parasites that produce spores. The infections caused by these mostly considered to be opportunistic in immunodeficient patients. Because of the zoonotic nature microsporidia as well increasing prevalence immunodeficiency diseases, aim this study was evaluate molecular diagnosis Enterocytozoon bieneusi (E. bieneusi) and Encephalitozoon spp. exotic birds southwestern Iran. Initially, 816 stool specimens were collected stained modified trichrome (Weber)...

Background . Rodents could act as reservoir for Cryptosporidium spp. specially C. parvum , a zoonotic agent responsible human infections. Since there is no information about infection in rodents of Ahvaz city, southwest Iran, hence, this survey was performed to determine the prevalence and molecular characterization region. Materials Methods One hundred were trapped from different regions city. Intestine contents fecal specimens studied using both microscopy examination identify oocyst...

Schizophrenia is a major psychiatric disorder with deeply destructive pathophysiology. There are evidences to indicate that infectious agents such as Toxoplasma gondii may play some roles in etiology of the disorder.The current study aimed determine association between T. exposure and risk schizophrenia.T. IgG antibodies 100 patients schizophrenia well 200 healthy volunteers were assessed. The subjects also completed demographic questionnaires. Data was analyzed using chi-square Fisher exact...

Dear Editor, Leishmaniasis is an important protozoan disease, which still a major health problem in endemic countries, such as Iran (1). Despite, recent advances, there long way until arriving to the ideal anti-leishmanial agents, with less toxicity, side effects and more potent efficacy. The World Health Organization (WHO) has suggested usage of herbal plants reach this aim (2). In Iranian folk medicine, Cordia myxa plant, belongs Boraginaceae family grows tropical regions, (3). Its...

Background: Nowadays, intestinal parasitic infections (IPIs) are one of the most common and serious public health problems, which affected 3.5 billion people worldwide, especially in developing countries. During time distribution, pattern disease may alter as a result changing life style residents. Objectives: The current study was aimed to determine prevalence IPIs town Haftkel, southwest Iran. Methods: In this descriptive cross-sectional study, 13698 stool specimens were collected during...

Leishmaniasis is a tropical parasitic infection. The resistance and toxicity issues are the major complications remain significant consequences related to treatment of leishmaniasis with recent classical drugs. Thus there an immediate requirement develop new compounds for this protozoan disease. Sea cucumbers or holothurians potentially presented as marine sources antimicrobial cytotoxic compounds. aim study was investigation in vitro antileishmanial activity methanol extract body wall,...

Apoptosis is the process of programmed cell death (PCD) that occurs in both animal and plant cells. Protozoan parasites possess metacaspase these caspase-related proteases could be involved PCD pathways organisms. Therefore we analyzed activities PARP genes Leishmania infantum (MCAN/IR/96/LON49) treated with miltefosine. Anti-leishmania activity miltefosine was studied by treatment cultured promastigotes various concentration MTT assay Annexin-V FLUOS staining using FACS flow cytometry...

Background: Schizophrenia is a major psychiatric disorder with deeply destructive pathophysiology. There are evidences to indicate that infectious agents such as Toxoplasma gondii may play some roles in etiology of the disorder. Objectives: The current study aimed determine association between T. exposure and risk schizophrenia. Materials Methods: IgG antibodies 100 patients schizophrenia well 200 healthy volunteers were assessed. subjects also completed demographic questionnaires. Data was...