A5037681679- Fatty Acid Research and Health
- Immune Response and Inflammation
- Axon Guidance and Neuronal Signaling
- Eicosanoids and Hypertension Pharmacology
- Peptidase Inhibition and Analysis
- Immune cells in cancer
- Mast cells and histamine
- Studies on Chitinases and Chitosanases
- Angiogenesis and VEGF in Cancer
- Apelin-related biomedical research
- Cancer, Hypoxia, and Metabolism
- Metabolomics and Mass Spectrometry Studies
- Advanced Glycation End Products research
- Invertebrate Immune Response Mechanisms
- Chemokine receptors and signaling
- Kruppel-like factors research
- Cancer Immunotherapy and Biomarkers
- Inflammatory Biomarkers in Disease Prognosis
- Stress Responses and Cortisol
- Neuropeptides and Animal Physiology
- Cholesterol and Lipid Metabolism
- Antimicrobial Peptides and Activities
- Adipokines, Inflammation, and Metabolic Diseases
- Biomarkers in Disease Mechanisms
- Immune Cell Function and Interaction
Brigham and Women's Hospital
2016-2025
Harvard University
2016-2025
Yale University
2024
Florida Atlantic University
2008-2020
Atlantic University
2010-2016
Proinflammatory eicosanoids (prostaglandins and leukotrienes) specialized pro-resolving mediators (SPM) are temporally regulated during infections. Here we show that human macrophage phenotypes biosynthesize unique lipid mediator signatures when exposed to pathogenic bacteria. E. coli S. aureus each stimulate predominantly proinflammatory 5-lipoxygenase (LOX) cyclooxygenase pathways (i.e., leukotriene B4 prostaglandin E2) in M1 macrophages. These pathogens M2 macrophages produce SPMs...
Resolution of acute inflammation is an active process orchestrated by endogenous mediators and mechanisms pivotal in host defense homeostasis. The macrophage mediator resolving inflammation, maresin 1 (MaR1), a potent immunoresolvent, stimulating resolution organ protection. Using unbiased screening greater than 200 GPCRs, we identified MaR1 as stereoselective activator for human leucine-rich repeat containing G protein-coupled receptor 6 (LGR6), expressed phagocytes. specificity recombinant...
Abstract Resolution of acute inflammation is an active process governed by specialized proresolving mediators, including resolvin (Rv)D2, that activates a cell surface G protein–coupled receptor, GPR18/DRV2. In this study, we investigated RvD2-DRV2–dependent resolution mechanisms using DRV2-deficient mice (DRV2-knockout [KO]). polymicrobial sepsis initiated cecal ligation and puncture, RvD2 (∼2.7 nmol/mouse) significantly increased survival (>50%) wild-type reduced hypothermia...
Chronic inflammation is a key pathological hallmark of multiple sclerosis (MS) and suggests that resolution inflammation, orchestrated by specialized pro-resolving lipid mediators (LM), impaired. Here, through targeted-metabololipidomics in peripheral blood patients with MS, we revealed each disease form was associated distinct LM profiles significantly correlated severity. In particular, relapsing progressive MS were high eicosanoids levels, whereas the majority reduced or below limits...
Abstract Disseminated metastasis accounts for over 90% of breast cancer deaths. Recently, elevated serum levels a glycoprotein known as chitinase‐3 like‐protein‐1 (CHI3L1) has been correlated with poor prognosis and shorter survival patients metastatic cancer. In this study, we show that there are increased CHI3L1 in plasma tumor‐bearing mice both tumor cells immune express secrete CHI3L1. However, the biological physiological functions still unclear. We demonstrate while an inhibitory role...
Physiologic hypoxia activates SPM and novel RvE4 biosynthesis that enhances clearance mechanisms.
The resolution of the acute inflammatory response is governed by phagocytes actively clearing apoptotic cells and pathogens. Biosynthesis specialized pro-resolving mediators (SPMs) pivotal in inflammation via their roles innate immune cells. Resolvin E4 (RvE4: 5S,15S-dihydroxy-eicosapentaenoic acid) a newly uncovered member E-series resolvins biosynthesized from eicosapentaenoic acid (EPA) recently elucidated physiologic hypoxia. This new resolvin was termed RvE4 given its ability to...
Semaphorins are a large family of molecules involved in axonal guidance during the development nervous system and have been recently shown to both angiogenic anti-angiogenic properties. Specifically, semaphorin 7A (SEMA7A) has reported chemotactic activity neurogenesis be an immune modulator through α1β1integrins. SEMA7A promote monocyte chemotaxis induce them produce proinflammatory mediators. In this study we explored role murine model breast cancer. We show that is highly expressed by...
Factors generated by blood coagulation that elicit immune responses protect the host are identified.
Acute inflammation is the body's first defense in response to pathogens or injury that partially governed by a novel genus of endogenous lipid mediators orchestrate resolution inflammation, coined specialized pro-resolving (SPMs). SPMs, derived from omega-3-polyunstaturated fatty acids (PUFAs), include eicosapentaenoic acid-derived and docosahexaenoic Resolvins, Protectins, Maresins. Herein, we review their biosynthesis, structural characteristics, therapeutic effectiveness various diseases...
Occupational exposure to engineered nanomaterials (ENMs) is increasing in the workplace and can impact human health. Amorphous silicon dioxide nanoparticles (SiO2 NPs) are widely produced respirable ENMs used commercial products. We have investigated their on lung inflammation resolution bacterial defense. Mice exposed SiO2 NPs, followed by bacteria, exhibited increased inflammation, proliferation, damage compared mice not NPs. NPs macrophage production of pro-inflammatory mediators...
Abstract Local production and downstream metabolism of specialized proresolving lipid mediators (SPMs) are pivotal in regulating their biological actions during resolution inflammation. Resolvin D4 (RvD4: 4S,5R,17S-trihydroxydocosa-6E,8E,10Z,13Z,15E,19Z hexaenoic acid) is one the more recently elucidated SPMs with complete stereochemistry biosynthesized from docosahexaenoic acid. Here, we report a new multimilligram commercial synthesis that afforded enough material for matching, validation,...
Significance Resolution of inflammation, infection, and injury are essential for host defense homeostasis. Cysteinyl-specialized proresolving mediators (cys-SPMs) potent chemical signals that accelerate resolution control display proregenerative properties. We sought evidence cys-SPM–activated primordial pathways might link inflammation regeneration using planaria because their robust regenerative ability. RNA sequencing was carried out with surgically resected exposed to cys-SPMs, we...
Solid tumors can generate a plethora of neurogenesis-related molecules that enhance their growth and metastasis. Among them, we have identified axonal guidance molecule Semaphorin 7A (SEMA7A) in breast cancer. The goal this study was to determine the therapeutic effect suppressing SEMA7A levels 4T1 murine model advanced carcinoma. We used anti-SEMA7A short hairpin RNA (shRNA) gene silence mammary tumor cells. When implanted into fat pads syngeneic mice, shRNA-expressing exhibited decreased...
Abstract The elusive nature of the liver immune system in newborns remains an important challenge, casting a shadow over our understanding how to effectively treat and prevent diseases children. Therefore, deeper exploration into intricacies neonatal immunology might be crucial for improved pediatric healthcare. Using intravital microscopy, we unveiled significant population granulocytes hepatic parenchyma fetuses newborns. Utilizing high-dimensional immunophenotyping, showed dynamic...
For either healthy or diseased organisms, lipids are key components for cellular membranes; they play important roles in numerous processes including cell growth, proliferation, differentiation, energy storage and signaling. Exercise disease development examples of environment alterations which produce changes these networks. There indications that lipid metabolism contribute to the progression a variety cancers. Measuring such understanding pathways involved is critical fully understand...