A5023940086- Single-cell and spatial transcriptomics
- Immune cells in cancer
- Gene expression and cancer classification
- Epigenetics and DNA Methylation
- Neuroinflammation and Neurodegeneration Mechanisms
- Cell Image Analysis Techniques
- Cancer Genomics and Diagnostics
- Analytical Chemistry and Chromatography
- Photosynthetic Processes and Mechanisms
- Microbial Metabolic Engineering and Bioproduction
The University of Melbourne
2011-2021
Stem Cells Australia
2020
Griffith University
2011
Stemformatics is an established gene expression data portal containing over 420 public datasets derived from microarray, RNA sequencing and single cell profiling technologies. Developed for the stem community, it has a major focus on pluripotency, tissue cells, staged differentiation. includes curated 'collections' of relevant to reprogramming, as well hematopoiesis leukaemia. Rather than simply rehosting they appear in repositories, uses stringent set quality control metrics its own...
Abstract Summary: Differential scanning fluorimetry (DSF) is a rapid technique that can be used in structural biology to study protein–ligand interactions. We have developed DMAN, novel tool analyse multi-well plate data obtained DSF experiments. DMAN easy install and provides user-friendly interface. Multi-well layouts designed by the user experimental annotated analysed according specified layout. Statistical tests for significance are performed automatically, graphical tools also provided...
The Stemformatics myeloid atlas is an integrated transcriptome of human macrophages and dendritic cells that systematically compares freshly isolated tissue-resident, cultured, pluripotent stem cell–derived cells. Three classes tissue-resident macrophage were identified: Kupffer microglia; monocyte-associated; tumor-associated macrophages. Culture had a major impact on all primary cell phenotypes. Pluripotent characterized by atypical expression collagen highly efferocytotic phenotype....
Gene expression atlases have transformed our understanding of the development, composition and function human tissues. New technologies promise improved cellular or molecular resolution, led to identification new cell types, better defined states. But as emerge, information derived on old platforms becomes obsolete. We demonstrate that it is possible combine a large number different profiling experiments summarised from dozens laboratories representing hundreds donors, create an integrated...
Summary The Stemformatics myeloid atlas is an integrated transcriptome of human macrophages and dendritic cells that systematically compares freshly isolated tissue-resident, cultured, stem-cell derived cell types. We identified two broad classes tissue-resident with lung, gut tumour-associated most similar to monocytes. Microglia, Kupffer synovial shared profiles each other, cultured macrophages. Pluripotent stem cell-derived were not reminiscent fetal-derived cells. Instead, they...
ABSTRACT Gene expression atlases have transformed our understanding of the development, composition and function human tissues. New technologies promise improved cellular or molecular resolution, led to identification new cell types, better defined states. But as emerge, information derived on old platforms becomes obsolete. We demonstrate that it is possible combine a large number different profiling experiments summarised from dozens laboratories representing hundreds donors, create an...