Abstract An intact mucus layer is important in managing inflammatory bowel disease (IBD). Dairy Propionibacterium freudenreichii has probiotic potential, produces propionic acid and known to promote health. The aim of this study was evaluate the effects P. on improvement colitis. LS 174T goblet cells a dextran sodium sulfate (DSS)-induced colitis rat model were used investigate -induced stimulation mucin production vitro vivo , respectively. mRNA protein expression levels MUC2, main...

Recent evidence shows that the gut microbiota has an important role in gut-brain crosstalk and is linked to neuronal disorders. The aim of this study was investigate effects intestinal Ruminococcus albus with probiotic potential on neuroprotection oxidatively stressed SH-SY5Y neuroblastoma cells animals. To these effects, conditioned medium prepared using Caco-2 cultured heat-killed R. (CRA-CM). showed increased BDNF expression protein levels CRA-CM. CRA-CM up-regulated SRF, C-fos CDK2. In...

Microbiota-derived short-chain fatty acids (SCFAs) are known to stimulate mucin expression in the intestine, which contributes gut mucosal immune responses, and system extends brain other organs through several axes. Hypoxia-inducible factors (HIFs), especially HIF-1α, act as master regulator of expression, however, underlying mechanism during hypoxia by SCFAs remains unclear. In this study, we investigated MUC2 propionate, an SCFA, intestinal goblet cells. The real time oxygen consumption...

Osteoblast differentiation is important for the development of bone and maintenance density. Propionibacterium freudenreichii a probiotic with an anti-inflammatory property. The aim this study was to investigate enhancement effect P. MJ2 (MJ2) isolated from raw milk on osteoblast differentiation, mineralization, its signaling pathway. For in vitro vivo experiments, human fetal osteoblastic cell line hFOB 1.19 ovariectomized rat model were used, respectively. Expression levels genes proteins...

The prevalence of gout is increasing worldwide, and control serum uric acid level has been regarded as one the therapeutic methods for gout. Inhibition xanthine oxidase (XO) activity which can oxidize hypoxanthine to commonly proposed decrease level. aim this study was demonstrate hypouricemic effect ethanol extract Aster glehni leaves (EAG) by in vitro vivo potassium oxonate (PO)-induced hyperuricemic rats. EAG possessed 132.5 ± 6.8 mg QE/g total flavonoid showed antioxidant activity....

Colitis causes destruction of the intestinal mucus layer and increases inflammation. The use antioxidants anti-inflammatory agents derived from natural sources has been recently highlighted as a new approach for treatment colitis. Oxyresveratrol (OXY) is an antioxidant known to have various beneficial effects on human health, such anti-inflammatory, antibacterial activity, antiviral activity. aim this study was investigate therapeutic effect OXY in rats with dextran sulfate sodium...

Osteoclast differentiation is crucial for bone absorption, and osteoclasts are involved in destruction rheumatoid arthritis (RA). Dairy Propionibacterium freudenreichii used as a cheese starter possesses prebiotic postbiotic properties. It known to stimulate the growth of bifidobacteria produces valuable metabolites, such vitamin B12 propionic acid. However, limited information available on beneficial effects P. human disease. Herein, we aimed investigate inhibitory effect MJ2 (MJ2) isolated...

Abstract Lipofuscin is a representative biomarker of aging that generated naturally over time. Remofuscin (soraprazan) improves age-related eye diseases by removing lipofuscin from retinal pigment epithelium (RPE) cells. In this study, the effect remofuscin on longevity in Caenorhabditis elegans and underlying mechanism were investigated. The results showed significantly ( p < 0.05) extended lifespan C. (N2) compared with negative control. Aging biomarkers improved remofuscin-treated...

Autophagy is a cell protection system invoked to eliminate the damaged organelles and misfolded proteins that induce various stresses, including endoplasmic reticulum (ER) stress. can control mucin secretion in goblet cells. Oxyresveratrol (OXY), an antioxidant, stimulates expression of MUC2. Thus, we investigated effect OXY on autophagy found OXY-induced MUC2 human intestinal cells.Autophagy-related genes were examined by quantitative real-time PCR (qPCR) Western blotting, respectively. was...

Osteoclasts degrade bone and osteoclast differentiation has been implicated in destruction rheumatoid arthritis. The dairy bacterium Propionibacterium freudenreichii MJ2 (MJ2) isolated from raw milk inhibits ameliorates collagen-induced This study aimed to investigate the inhibitory effect of surface proteins on receptor activator nuclear factor-κB ligand (RANKL)-induced explain underlying mechanism. murine macrophage cell line RAW 264.7 was used inhibition differentiation. significantly...

Background: MUC2, a major component of the mucus layer in intestine, is associated with antimicrobial activity and gut immune system function. Currently, mucin mainly known for its critical function defense against toxic molecules pathogens. In this study, we investigated stimulatory effects exogenous nicotinamide adenine dinucleotide (NAD+) on expression MUC2 LS 174T goblet cells. Methods: Genes related to synthesis were measured by quantitative real-time PCR (qPCR). To analyze gene...

Canine mammary tumors (CMTs) are commonly observed in old and unspayed female dogs. Recently, dogs have been increasingly spaying at a young age to prevent tumors. These CMTs require extensive local excision exhibit high probability of metastasis the regional lymph nodes lungs during malignancy. However, molecular biological mechanisms underlying CMT development not fully elucidated, research this area is limited. Therefore, study, we established new cell lines by isolating cells from tumor...