A5059609093- Retinal Development and Disorders
- Congenital heart defects research
- CRISPR and Genetic Engineering
- Advanced Fluorescence Microscopy Techniques
- Retinal and Optic Conditions
- Single-cell and spatial transcriptomics
- Congenital Heart Disease Studies
- Retinal Diseases and Treatments
- Genetics and Neurodevelopmental Disorders
- RNA Research and Splicing
- RNA regulation and disease
- Genomics and Chromatin Dynamics
University of California, Berkeley
2022-2025
University of Washington
2020-2023
Highlights•scRNA-seq of human retina highlights key developmental transition states•hPSC-derived retinal organoids mimic fetal cellular composition•Inner layers in exhibit differences gene expression and organization•Fetal cultures resemble but maintain better inner laminationSummaryTo study the development retina, we use single-cell RNA sequencing (RNA-seq) at stages follow major cell types as well populations transitional cells. We also analyze stem (hPSC)-derived organoids; although have...
Regenerative neuroscience aims to stimulate endogenous repair in the nervous system replace neurons lost from degenerative diseases. Recently, we reported that overexpressing transcription factor Ascl1 Müller glia (MG) is sufficient MG regenerate functional adult mouse retina. However, this process inefficient, and only a third of Ascl1-expressing generate new neurons. Here, test whether proneural factors Atoh1/7 class can further promote regenerative capacity MG. We find combination...
In mammals, loss of retinal cells due to disease or trauma is an irreversible process that can lead blindness. Interestingly, regeneration neurons a well established in some non-mammalian vertebrates and driven by the Müller glia (MG), which are able re-enter cell cycle reprogram into neurogenic progenitors upon injury disease. Progress has been made restore this mechanism mammals promote regeneration: MG be stimulated generate new vivo adult mouse retina after over-expression pro-neural...
ABSTRACT During vertebrate development, the heart primarily arises from mesoderm, with crucial contributions cardiac neural crest cells that migrate to and form a variety of cardiovascular derivatives. Here, by integrating bulk single cell RNA-seq ATAC-seq, we identify gene regulatory subcircuit specific migratory composed key transcription factors egr1, sox9a, tfap2a ets1 . Notably, show expressing canonical sox10 are essential for proper regeneration in adult zebrafish. Furthermore,...