A5102748010- Gut microbiota and health
- Tryptophan and brain disorders
- Stress Responses and Cortisol
- Maternal Mental Health During Pregnancy and Postpartum
- Neuroendocrine regulation and behavior
- Clostridium difficile and Clostridium perfringens research
- Diet and metabolism studies
- Gastrointestinal motility and disorders
- Nutritional Studies and Diet
- Probiotics and Fermented Foods
- Food Security and Health in Diverse Populations
- Infant Nutrition and Health
- Birth, Development, and Health
- Reproductive System and Pregnancy
- Natural Compound Pharmacology Studies
- Hormonal Regulation and Hypertension
- Pediatric health and respiratory diseases
- Microscopic Colitis
- Infant Health and Development
- Medical Imaging and Analysis
- Inflammatory Bowel Disease
- Extracellular vesicles in disease
- Immune cells in cancer
- COVID-19 Impact on Reproduction
- Health, psychology, and well-being
The Ohio State University Wexner Medical Center
2014-2024
Institute for Behavioral Medicine
2011-2024
The Ohio State University
2010-2023
Nationwide Children's Hospital
2020
Baylor College of Medicine
2016-2017
Ohio University
2010-2016
Fribourg Development Agency
2016
Columbus Oncology and Hematology Associates
2014
The microbiota of the mammalian gastrointestinal (GI) tract consists diverse populations commensal bacteria that interact with host physiological function. Dysregulating these populations, through exogenous means such as antibiotics or dietary changes, can have adverse consequences on health host. Studies from laboratories ours demonstrated exposure to psychological stressors disrupts population profile intestinal microbiota. To date, studies primarily focused prolonged (repeated across...
ABSTRACT The gastrointestinal tract is colonized by an enormous array of microbes that are known to have many beneficial effects on the host. Previous studies indicated stressor exposure can disrupt stability intestinal microbiota, but extent these changes, as well enteric infection, has not been characterized. In order examine ability stressors induce changes in gut we exposed mice a prolonged restraint and then characterized microbial populations intestines using both traditional culture...
There is a groundswell of interest in using genetically engineered sensor bacteria to study gut microbiota pathways, and diagnose or treat associated diseases. Here, we computationally identify the first biological thiosulfate an improved tetrathionate sensor, both two-component systems from marine Shewanella species, validate them laboratory Escherichia coli Then, port these sensors into gut-adapted probiotic E. strain, develop method based upon oral gavage flow cytometry colon fecal...
Children born to obese mothers are at increased risk for obesity, but the mechanisms behind this association not fully delineated. A novel possible pathway linking maternal and child weight is transmission of obesogenic microbes from mother child. The current study examined whether obesity was associated with differences in composition gut microbiome children early life. Fecal samples 18–27 months age (n = 77) were analyzed by pyro-tag 16S sequencing. Significant effects on offspring...
The commensal microbiota of the human gastrointestinal tract live in a largely stable community structure, assisting host physiological and immunological functions. Changes to this structure can be injurious health host, concept termed dysbiosis. Psychological stress is factor that has been implicated causing dysbiosis, studies performed by our lab have shown restraint indeed shift cecal as well increase severity colonic infection caused Citrobacter rodentium. However, study, like many...
Psychosocial stress and mood-related disorders, such as depression, are prevalent vulnerability to these conditions is heightened during pregnancy. induces consequences via several mechanisms including the gut microbiota-brain axis associated signaling pathways. Previous preclinical work indicates that prenatal alters maternal microbial composition impairs offspring development. Importantly, although fecal vaginal microenvironments undergo alterations across pregnancy, we lack consensus...
Scope Ulcerative colitis (UC) is a chronic inflammatory disease of the colon. α-Mangostin (α-MG), most abundant xanthone in mangosteen fruit, exerts anti-inflammatory and antibacterial activities vitro. We evaluated impact dietary α-MG on murine experimental gut microbiota healthy mice. Methods results Colitis was induced C57BL/6J mice by administration dextran sulfate sodium (DSS). Mice were fed control diet or with (0.1%). exacerbated pathology DSS-induced colitis. had greater colonic...
Abstract Maternal stress during pregnancy is widespread and associated with poor offspring outcomes, including long-term mental health issues. Prenatal stress-induced fetal neuroinflammation thought to underlie aberrant neurodevelopment derive from a disruption in intrauterine immune homeostasis, though the exact origins are incompletely defined. We aimed identify divergent microbial metagenome profiles of stressed gestating mice that may trigger detrimental inflammatory signaling at...
Stressor exposure significantly affects the colonic mucosa-associated microbiota, and exacerbates Citrobacter rodentium-induced inflammation, effects that can be attenuated with probiotic Lactobacillus reuteri. This study assessed structure of microbiota in mice exposed to a social stressor (called disruption), as well non-stressed control mice, during challenge pathogen C. rodentium. Mice were or home cage conditions for six consecutive days all challenged rodentium immediately following...
Microleakage through the interface has been documented in implant systems with titanium (Ti) abutments. There is a current increase use of zirconia (Zi) abutments especially esthetic zone where higher risk visible metal color peri-implant tissues exists.The aim present vitro study to evaluate leakage at fixture-abutment two different screw-retained abutment torque values nonloading condition.In design included four groups (Ti and Zi torqued 25 15 Ncm [N = 8/group]). Microcomputed tomography...
For prophylactic therapy, mice received an oral antibiotic cocktail followed by clindamycin injection, probiotic administration (planktonic vs. biofilm state), C. difficile gavage. treatment antibiotics and first, administration. Clinical sickness scores (CSS) intestinal histologic injury (HIS) were assigned.In the Prophylactic Therapy model, CSS: 67% of untreated exposed to demonstrated CSS ≥ 6, which is consistent with infection (p< .001 compared unexposed mice). In treated planktonic Lr,...
Beverages and supplements prepared from mangosteen fruit are claimed to support gut health immunity, despite the absence of supporting evidence clinical trials. We recently reported that α-mangostin (α-MG), most abundant xanthone in fruit, altered intestinal microbiome, promoted dysbiosis, exacerbated colitis C57BL/6J mice. The objective this study was determine whether induction dysbiosis by dietary α-MG is limited strain or represents a more generic response chronic intake on microbiota...
The complex and dynamic interactions between diet, gut microbiota (GM) structure function, colon carcinogenesis are only beginning to be elucidated. We examined the colonic aberrant crypt foci (ACF) in C57BL/6N female mice fed various dietary interventions (control, energy restricted high-fat) provided during two phases (initiation progression) of azoxymethane (AOM)-induced early carcinogenesis. During progression (wks. 22-60), a high-fat diet enhanced ACF formation compared control or diet....