A5001135432- Ruminant Nutrition and Digestive Physiology
- Gut microbiota and health
- Anaerobic Digestion and Biogas Production
- Genomics and Phylogenetic Studies
- Microbial Community Ecology and Physiology
- Biofuel production and bioconversion
- Plant and fungal interactions
- Reproductive Physiology in Livestock
- Genetic and phenotypic traits in livestock
- Clostridium difficile and Clostridium perfringens research
- Probiotics and Fermented Foods
- Pharmaceutical and Antibiotic Environmental Impacts
- Microbial bioremediation and biosurfactants
- Bacteriophages and microbial interactions
- Animal health and immunology
- Molecular Biology Techniques and Applications
- Gout, Hyperuricemia, Uric Acid
- Microbial Fuel Cells and Bioremediation
- Turfgrass Adaptation and Management
- Protist diversity and phylogeny
- Tea Polyphenols and Effects
- Microbial Metabolic Engineering and Bioproduction
- Wastewater Treatment and Nitrogen Removal
- Agriculture Sustainability and Environmental Impact
- Effects of Environmental Stressors on Livestock
The Ohio State University
2016-2025
Zhejiang University
2025
Yanbian University
2025
Institute of Animal Sciences
2012-2024
Jinan University
2023-2024
Hubei Academy of Agricultural Sciences
2024
Animal Welfare Institute
2019
Chinese Academy of Agricultural Sciences
2018
Columbus Oncology and Hematology Associates
2013
Graduate School USA
2012
Several DNA extraction methods have been reported for use with digesta or fecal samples, but problems are often encountered in terms of relatively low yields and/or recovering free inhibitory substances. Here we report a modified method to extract PCR-quality microbial community from these types which employs bead beating the presence high concentrations sodium dodecyl sulfate (SDS), salt, and EDTA, subsequent purification by QIA® columns [referred as repeated plus column (RBB+C) method]....
The objective of this study was to generate a phylogenetic diversity census bacteria identified in the intestinal tract chickens and turkeys using naïve analysis all curated 16S rRNA gene sequences archived public databases. High-quality chicken turkey gastrointestinal origin (3,184 1,345, respectively) were collected from GenBank, Ribosomal Database Project, Silva comprehensive ribosomal RNA database. Through statistical analysis, 915 464 species-equivalent operational taxonomic units...
ABSTRACT Denaturing gradient gel electrophoresis (DGGE) has become a widely used tool to examine microbial diversity and community structure, but no systematic comparison been made of the DGGE profiles obtained when different hypervariable (V) regions are amplified from same DNA samples. We report here study make such comparisons establish preferred choice V region(s) by DGGE, extracted samples digesta is used. When members phylogenetically representative set 218 rrs genes archived in RDP II...
In this study, the collective microbial diversity in rumen was examined by performing a meta-analysis of all curated 16S rRNA gene (rrn) sequences deposited RDP database. As November 2010, 13,478 bacterial and 3516 archaeal rrn were found. The assigned to 5271 operation taxonomic units (OTUs) at species level (0.03 phylogenetic distance) representing 19 existing phyla, which Firmicutes (2958 OTUs), Bacteroidetes (1610 OTUs) Proteobacteria (226 most predominant. These grouped into more than...
The microbiota of the mammalian gastrointestinal (GI) tract consists diverse populations commensal bacteria that interact with host physiological function. Dysregulating these populations, through exogenous means such as antibiotics or dietary changes, can have adverse consequences on health host. Studies from laboratories ours demonstrated exposure to psychological stressors disrupts population profile intestinal microbiota. To date, studies primarily focused prolonged (repeated across...
ABSTRACT Five essential oils (EOs), namely, clove oil (CLO), eucalyptus (EUO), garlic (GAO), origanum (ORO), and peppermint (PEO), were tested in vitro at 3 different doses (0.25, 0.50, 1.0 g/liter) for their effect on methane production, fermentation, select groups of ruminal microbes, including total bacteria, cellulolytic archaea, protozoa. All the EOs significantly reduced production with increasing doses, reductions by 34.4%, 17.6%, 42.3%, 87%, 25.7% CLO, EUO, GAO, ORO, PEO,...
A custom phylogenetic microarray composed of small subunit ribosomal RNA probes, representing ≈500 bacterial species from the human and animal gut, was developed evaluated for analysis gut microbial diversity using fecal samples healthy subjects Crohn's disease (CD) patients.Oligonucleotide probes (≈40 mer) used on were selected published articles or designed with "GoArray" probe design program 16S rRNA sequences. Fecal rDNA individual six CD patients as template to generate fluorescently...
The trillions of microbes that exist in the gastrointestinal tract have emerged as pivotal regulators mammalian development and physiology. Disruption this gut microbiome, a process known dysbiosis, causes or exacerbates various diseases, but whether dysbiosis affects recovery neurological function lesion pathology after traumatic spinal cord injury (SCI) is unknown. Data study show SCI increases intestinal permeability bacterial translocation from gut. These changes are associated with...
Significance Agricultural methane emissions must be decreased by 11 to 30% of the 2010 level 2030 and 24 47% 2050 meet 1.5 °C target. We identified three strategies decrease product-based while increasing animal productivity five absolute without reducing productivity. Globally, 100% adoption most effective emission mitigation strategy can target but not 2050, because effects are offset projected increases in methane. On a regional level, Europe Africa may able their contribution target,...
Poultry litter is a mixture of bedding materials and enteric bacteria excreted by chickens, it typically reused for multiple growth cycles in commercial broiler production. Thus, can be transmitted from one cycle to the next via litter. However, remains poorly understood how reuse affects development composition chicken gut microbiota. In this study, effect on microbiota was investigated using 2 management regimens: fresh vs. Samples ileal mucosa cecal digesta were collected young chicks (10...
Abstract The diverse rumen virome can modulate the microbiome, but it remains largely unexplored. Here, we mine 975 published metagenomes for viral sequences, create a global database (RVD), and analyze diversity, virus-host linkages, potential roles in affecting functions. Containing 397,180 species-level operational taxonomic units (vOTUs), RVD substantially increases detection rate of viruses from compared with IMG/VR V3. Most classified vOTUs belong to Caudovirales , differing those...
ABSTRACT Thepotential for biodegradation of polycyclic aromatic hydrocarbons (PAHs)at low temperature and under anaerobic conditions is not wellunderstood, but such would be very useful forremediation polluted sites. Biodegradation a mixture 11different PAHs with two to five rings, each at concentrationof 10 μg/ml, was studied in enrichment cultures inoculated withsamples four northern soils. Under aerobic conditions, lowtemperature severely limited PAH biodegradation. After 90 days,...
ABSTRACT Erythromycin and tylosin are commonly used in animal production, such use is perceived to contribute the overall antimicrobial resistance (AR) reservoirs. Quantitative measurements of this type AR reservoir microbial communities required understand ecology (e.g., emergence, persistence, dissemination). We report here development, validation, six real-time PCR assays for quantifying classes erm genes (classes A through C, F, T, X) that encode major mechanism...
Abstract In microbial fuel cells (MFCs) bacteria generate electricity by mediating the oxidation of organic compounds and transferring resulting electrons to an anode electrode. The objective this study was test possibility generating with rumen microorganisms as biocatalysts cellulose electron donor in two‐compartment MFCs. cathode chambers were separated a proton exchange membrane graphite plates used electrodes. medium chamber inoculated microorganisms, catholyte compartment ferricyanide...
We report here the development, validation, and use of three real-time PCR assays to quantify abundance following groups tetracycline resistance genes: tet(A) tet(C); tet(G); tet genes encoding ribosomal protection proteins, including tet(M), tet(O), tetB(P), tet(Q), tet(S), tet(T), tet(W). The were validated using known numbers sample-derived gene templates added microbiome DNA. These are both precise accurate over at least 6 log copies. New variants also identified from cloned amplicons as...
The intestinal microbiota of broiler chickens and the in litter have been well studied, but interactions between these two microbiotas remain to be determined. Therefore, we examined their reciprocal effects by analyzing broilers reared on fresh pine shavings versus reused litter, as over a 6-week cycle. Composite ileal mucosal cecal luminal samples from birds (n = 10) with both conditions (fresh reused) were collected at 7, 14, 21, 42 days age. Litter also 42. profiled compared within...