A5004944363- Brucella: diagnosis, epidemiology, treatment
- Salmonella and Campylobacter epidemiology
- Microbial infections and disease research
- Bacteriophages and microbial interactions
- Mycobacterium research and diagnosis
- Escherichia coli research studies
- Reproductive tract infections research
- Animal Diversity and Health Studies
- Plant and fungal interactions
- Galectins and Cancer Biology
- Burkholderia infections and melioidosis
- Diphtheria, Corynebacterium, and Tetanus
- Coccidia and coccidiosis research
- RNA and protein synthesis mechanisms
- Monoclonal and Polyclonal Antibodies Research
- Bacterial Identification and Susceptibility Testing
- Animal Behavior and Welfare Studies
- Reproductive Physiology in Livestock
- Infectious Diseases and Mycology
- RNA regulation and disease
- Aquaculture disease management and microbiota
- Animal Disease Management and Epidemiology
- Prion Diseases and Protein Misfolding
- Immunotherapy and Immune Responses
- Virus-based gene therapy research
Universidad Nacional Autónoma de México
2014-2024
Hospital General Dr. Manuel Gea Gonzalez
2021
Fundação de Estudo e Pesquisa em Medicina Veterinária e Zootecnia
2021
Weatherford College
2021
Instituto Nacional de Investigaciones Forestales Agrícolas y Pecuarias
1999-2008
Inserm
2003-2005
Centre d’Immunologie de Marseille-Luminy
2003
Centre National de la Recherche Scientifique
2003
Dr. Hari Singh Gour University
1999
The virB locus, which encodes for the type IV secretion system, is a major component of virulence in Brucella. A non-polar virB10 mutant and virB11 deletion were constructed Brucella canis. In mouse model both mutants cleared at day 21 p.i., indicating reduced mice. After challenge with wild B. canis amount CFU recovered 15 was significantly lower group previously vaccinated mutant. Levels IgG1, IgG2a, IgG2b IgM as well induction cytokines IL-2, IL-4, IL-10 IFN- production, measured...
The in vivo efficacy of rifampicin encapsulated poly(lactic-co-glycolic acid) (PLGA) nanoparticles was evaluated for the treatment BALB/c mice experimentally infected with Brucella canis. PLGA loaded (RNP) were prepared using single emulsification-solvent evaporation technique, resulting a hydrodynamic diameter 138 ± 6 nm. zeta potential and polydispersity index values indicated that system relatively stable narrow size distribution. release from studied phosphate buffer at pH 7.4 37 °C....
Corynebacterium pseudotuberculosis is a pathogenic bacterium which has been rapidly spreading all over the world, causing economic losses in agricultural sector and sporadically infecting humans. Six C. strains were isolated from goats, sheep, horses with distinct abscess locations. For first time, Mexican genomes of this sequenced studied silico. All using Ion Personal Genome Machine sequencer, assembled Newbler SPAdes software. The automatic genome annotation was done software RAST...
Some Brucella isolates are known to require an increased concentration of CO2 for growth, especially in the case primary cultures obtained directly from infected animals. Moreover, different species and biovars show a characteristic pattern requirement, this trait has been included among routine typing tests used biovar differentiation. By comparing differences gene content CO2-dependent CO2-independent strains, we have confirmed that carbonic anhydrase (CA) II is enzyme responsible...
Brucellosis is a zoonotic infection caused by the consumption of contaminated raw milk and dairy products. This study aims to compare survival rates Brucella abortus RB51 S19 vaccine strains that virulent B. 2308 strain during manufacture fresh ripened cheeses. To do this, we inoculated pasteurized with RB51, S19, or at 6 × 108 colony-forming unit per milliliter concentration cheese making process. Cheese was manufactured room temperature, then, cheeses were conserved either 4°C 25°C for 7...
The objective of this work was to evaluate the survival a Brucella abortus aqpX mutant during elaboration and conservation fresh ripened cheeses at 4 °C 24 °C. pH values water activity were monitored for each type cheese. cheese elaborated with raw milk inoculated 6×10⁸ colony-forming units (CFU)/mL parental strain. Ripening both pasteurized 12×10⁸ CFU/mL strains. In cheese, observed 7 days in number survivors strain 10 times lower compared 5 a(w) 0.930. °C, strains survived until day 17 4.0...
Similar to what has been described in other Gram-negative bacteria, B. melitensis releases outer membrane vesicles (OMVs). OMVs from 16M and the rough-mutant VTRM1 were able induce a protective immune response mice. Some proteins which had previously reported protection against Brucella found proteome of 16M. However, not determined. In order be better understand role host-cell interactions, aim this work was compare proteomes derived VTRM1, as well characterize induced by on host cells....
Some of the mechanisms underlying invasion and intracellular survival B. melitensis are still unknown, including role a subfamily NUDIX enzymes, which have been described in other bacterial species as invasins present Brucella spp. We generated mutation coding gene one these proteins, invA (BMEI0215) strain 133, to understand its virulence. HeLa cell results showed that mutant was decreased 5-fold compared with parental at 2 h pi (P < 0.001). In goat macrophage infection assay, replication...
Currently, Salmonella spp. is the bacterium causing highest number of food-borne diseases (FADs) in world. It primarily associated with contaminated water used to that irrigates crops from intensive livestock farming. However, literature emphasizes reservoirs for remain wildlife and there are unconventional sources or secondary reservoirs, such as soil. Human soil-borne have not been modeled spatial scenarios, therefore it necessary consider soil other climatic factors anticipate emergence...
Although Chlamydia abortus is classified as an exotic agent in Mexico, there increasing evidence of its presence. The objective this study was to isolate C. dairy goat herds with problems abortion the state Guanajuato, and develop appropriate diagnostic methods for detection. Serological samples vaginal swabs were taken from 6 herds. ELISA revealed a seropositivity 9.60% abortus. PCR test based on mucus resulted 30 126 positive animals (23.8%). spp. isolated 34 tested (26.98%). 3 valuable...
Con el objetivo de inducir la infeccion experimental A. seminis, se utilizaron 18 corderos seis meses edad: 4 testigos negativos, otros 3 inocularon con seminis via intraepididimal (IE) y 11 fueron inoculados por intrauretral (IU) como sigue: cuatro recibieron factor liberador gonadotropinas (GnRH) cinco dias previos al desafio, etilenglicol IE 24 h previas desafio tres no tratamiento previo. De los dos GnRH etilenglicol. Se tomaron muestras semanales suero para inmunodifusion, semen...
Summary Two Quarter Horse stallions from the same region in Mexico presented with pectoral abscesses and lameness. Exudate samples were taken drained muscular Corynebacterium pseudotuberculosis , identified by bacteriological techniques multiplex polymerase chain reaction, was obtained a pure isolation culture both samples. The sequences of hypervariable rpoB gene showed 100% 99% identity to C. 316 biovar equi ( CP 003077.1) isolated horse. Both strains' located phylogenetic cluster as other...
The milk ring test is a detection assay for antibodies against Brucella in bovine milk. It has good sensitivity but tends to give false positive results. In this study, we standardized the application of fluorescence polarization (FPA) B.melitensis goat We obtained negative serum and samples from healthy flocks northern zone Nuevo León. Positive negative, weak, strong controls were by mixing volumes control with Milk treated citric acid, after which an FPA was performed. Results then...