A5070869715- Neuroscience and Neuropharmacology Research
- Alzheimer's disease research and treatments
- Memory and Neural Mechanisms
- Antibiotics Pharmacokinetics and Efficacy
- Pharmacological Receptor Mechanisms and Effects
- RNA regulation and disease
- Cancer therapeutics and mechanisms
- Prion Diseases and Protein Misfolding
- Pharmacological Effects and Toxicity Studies
- Photodynamic Therapy Research Studies
- Neuroscience and Neural Engineering
- Phytochemistry and Biological Activities
- Lung Cancer Research Studies
- Metabolomics and Mass Spectrometry Studies
- Photoacoustic and Ultrasonic Imaging
- Civil and Geotechnical Engineering Research
- Nonlinear Optical Materials Research
- Plant-based Medicinal Research
- Pharmacogenetics and Drug Metabolism
- Peroxisome Proliferator-Activated Receptors
- Hepatocellular Carcinoma Treatment and Prognosis
- Pneumonia and Respiratory Infections
- Drug-Induced Hepatotoxicity and Protection
- Cardiac tumors and thrombi
- Bone and Joint Diseases
Southwest Jiaotong University
2024
China Medical University
2011-2022
Liaoning Cancer Hospital & Institute
2014-2022
Trinity College Dublin
2014-2021
University of Science and Technology of China
2019
Shenyang Pharmaceutical University
2000-2012
Shanghai Medical College of Fudan University
2008
Neuronal network dysfunction is a hallmark of Alzheimer's disease (AD). However, the underlying pathomechanisms remain unknown. We analyzed hippocampal micronetwork in transgenic McGill-R-Thy1-APP rats (APPtg) at beginning extracellular amyloid beta (Aβ) deposition. established two-photon Ca2+ -imaging vivo hippocampus and found hyperactivity CA1 neurons. Patch-clamp recordings brain slices vitro revealed increased neuronal input resistance prolonged action potential width pyramidal did...
Long before synaptic loss occurs in Alzheimer's disease significant harbingers of may be detected at the functional level. Here we examined if long-term potentiation is selectively disrupted prior to extracellular deposition Aß a very complete model amyloidosis, McGill-R-Thy1-APP transgenic rat. Longitudinal studies freely behaving animals revealed an age-dependent, relatively rapid-onset and persistent inhibition without change baseline transmission CA1 area hippocampus. Thus ability...
10-hydroxy camptothecin (10-HCPT) is an antitumor agent effective in the treatment of several solid tumors but its use hampered by poor water solubility, low lactone stability, short plasma half-life and dose-limiting toxicity. In this study, 10-HCPT-hydroxyethyl starch (HES) conjugate was prepared to overcome these limits 10-HCPT. The solubility 10-HCPT 0.72 mg/ml, about 100 times free showed good sustained release effect phosphate-buffered saline (PBS), rat liver homogenate. Meanwhile,...
Abstract Synaptic dysfunction is a likely proximate cause of subtle cognitive impairment in early Alzheimer’s disease. Soluble oligomers are the most synaptotoxic forms amyloid ß-protein (Aß) and mediate synaptic plasticity disruption disease amyloidosis. Because presence extent cortisol excess prodromal predicts onset symptoms we hypothesised that corticosteroids would exacerbate inhibition hippocampal long-term potentiation rat model In longitudinal experimental design using freely...
Purpose: We developed and validated an ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method for simultaneous therapeutic drug monitoring (TDM) clinical pharmacokinetic antipsychotic drugs: clozapine (CLP), chlorpromazine (CPZ), risperidone (RPD), paliperidone (PLD), quetiapine (QTP;), aripiprazole (APZ), dehydroaripiprazole (DAP), olanzapine (OZP), ziprasidone (ZRD), amisulpride (ASP). Methods: Analytes internal standards (ISs) were separated using a...
A carbody swaying with low frequency of about 2 Hz occurs during vehicles linear induction motors (LIM) running in shallow curve and straight sections on a China metro line, which significantly deteriorates the ride comfort passengers. series field tests were carried out to identify main cause possible solutions. During tests, vibration characteristics, dynamic performance, wheel–rail contact condition track analysed. The test results show that abnormal is manifestation coupled resonance...
Human cytomegalovirus (HCMV) is a double-stranded DNA virus with the largest genome (~235 kb) of known human herpes viruses.The coding potential and transcript structures most HCMV predicted genes have not been identified.New or unknown could exist in clinical strains.The SMART (switching mechanism at 5ꞌ end RNA template reverse transcriptase) technique was used to construct full-length cDNA library an strain late expression phase.Randomly selected clones were sequenced.The sequenced...
Dehydroepiandrosterone sulfate (DHEAS) is one of the most important neuroactive steroids. The present study examined effect DHEAS on spontaneous and evoked glutamate release in pyramidal cells layers V VI rat prelimbic cortex by using whole-cell patch-clamp recordings slices further investigated its mechanism. results showed that at 1 μM had no but inhibited 5-hydroxytryptaime (5-HT)-evoked release. concentration-response relationship this was U-shaped with a maximum μM, inhibition seemed to...
How endogenously produced soluble amyloid ß-protein (Aß) affects synaptic plasticity in vulnerable circuits should provide insight into early Alzheimer's disease pathophysiology. McGill-R-Thy1-APP transgenic rats, modeling amyloidosis, exhibit an age-dependent Aß-mediated impairment of the induction long-term potentiation (LTP) by 200 Hz conditioning stimulation at apical CA3-to-CA1 synapses. Here, we investigated if weakening these synapses form activity-dependent persistent reversal...
10-HCPT is a topoisomerase I inhibitor effective in the treatment of liver cancer but its use hampered by resistance. The expression hypoxia-inducible factor-1α (HIF-1α) reportedly upregulated tissues, which directly linked to resistance 10-HCPT. While BBR can significantly decrease level HIF-1α according literature report. Thus, aim this study was prepare novel intravenous 10-HCPT-BBR-loaded lipid microsphere (LM) and evaluate their synergistic effect on treatment. optimal preparation...
Multifunctional nanoparticles with targeted therapeutic function and diagnostic-imaging are of great interest in the domain precision therapy. NIR laser responsive (PLGA-PEG-FA encapsulating Bi2S3, PFP, Dox (designed as FBPD NPs)) synthesized for ovarian cancer combination therapy CT/PA dual-modal imaging guidance (PA: photoacoustic; CT: X-ray computed tomography).The NPS prepared by double emulsification method revealed excellent dispersity, stability, outstanding optical properties. The...
A reversed-phase high-performance liquid chromatographic method is developed for the determination of astragaloside (AGS) IV, which known as active constituent Radix Astragali. The uses precolumn derivatization with benzoyl chloride to form ester AGS IV quantitatively and carried out a wide-ranging concentration (0.004-0.080 mg/mL) derivatized IV. eluent consists 90% methanol, 4% tetrahydrofuran, 6% water, 0.2% triethylamine, vitamin D3 added internal standard.
Pregnenolone sulphate (PREGS) is one of the most important neuroactive steroids. Previous study showed that PREGS enhanced long-term potentiation (LTP) via activation post-synaptic NMDA receptors at excitatory synapses in hippocampus. The present paper studied effect on LTP pyramidal cells layers V–VI medial prefrontal cortex (mPFC) using whole-cell patch-clamp slices and made a comparison with We also mechanism mPFC. found inhibited induction mPFC had no influence currents, which was...
Hepatocellular carcinoma (HCC) is a complicated and poor prognosis cancer, necessitating the development of potential treatment strategy. In this study, we initially revealed that LukS-PV belonged to leukocidin family performs an anti-HCC action. Then, used liquid chromatography-mass spectrometry (LC/MS) compare protein expression profiles LukS-PV-treated human HCC cell lines HepG2 control cells. GO annotations Kyoto Encyclopedia Genes Genomes pathway analysis were carried out differential...
Abstract With the purpose of carrying out therapeutic drug monitoring (TDM) nine antiepileptic drugs simultaneously in clinic, a UPLC–MS/MS assay method was developed and validated. Separation internal standard (naproxen diazepam) performed on an Agilent Eclipse XDB‐C 18 column (50.0 × 2.1 mm, 1.7 μm) using water acetonitrile as mobile phase for gradient elution. Polarity switch ionization mode (positive–negative–positive) total run time 3.0 min. The validation conducted based bioanalytical...
Abstract Neuronal network dysfunction is a hallmark of Alzheimer’s disease (AD). However, the underlying pathomechanisms remain unknown. We analyzed hippocampal micronetwork in rat model AD at an early stage beginning extracellular amyloid beta (Aβ) deposition. established two-photon Ca 2+ -imaging vivo hippocampus rats and found hyperactivity CA1 neurons. Patch-clamp recordings brain slices vitro revealed changes passive properties intrinsic excitability pyramidal Furthermore, we observed...
A simple and specific LC-MS/MS method for the determination of FK506 in blood was developed, validated, successfully applied pharmacokinetic studies effect silymarin on rats. Blood samples were extracted by liquid-liguid extraction with methyl tert-butyl ether separated an Eclipse plus-C18 column (150 × 2.1 mm, 3.5 µm) mobile phase acetonitrile-10 mM ammonium acetate buffer solution (80:20, v/v). detected without interference multiple-reaction monitoring mode positive electrospray...
Recently, we showed that an Aß-mediated, age-dependent, inhibition of long-term potentiation (LTP) in McGill-R-Thy1-APP transgenic rats could be overcome by increasing the strength conditioning stimulation (Qi et al., Acta Neuropath. Comm., 2014). Here, investigated effect APP overexpression on depotentiation previously established LTP, a form synaptic plasticity also believed to underpin successful memory function. In vivo experiments were carried out both acutely urethane-anaesthetized and...
Abstract Background Growing evidence shows that targeting the integrated stress response (ISR) through inhibition of phosphorylation eIF2α provides beneficial effects in animal models neurodegenerative diseases including Alzheimer’s disease (AD). However, those results are inconsistent and somehow conflicting likely due to important role ISR both cell death survival. Aβ-triggered pathologies microvascular hypoxia, neuronal hyperactivation, neuroinflammation common inducers ISR. The...