A5090558107- RNA Research and Splicing
- RNA and protein synthesis mechanisms
- RNA modifications and cancer
- Cancer-related molecular mechanisms research
- RNA regulation and disease
- Bacteriophages and microbial interactions
- Viral Infections and Immunology Research
- Legume Nitrogen Fixing Symbiosis
- Genetic Syndromes and Imprinting
- Genomics and Phylogenetic Studies
European Molecular Biology Laboratory
2019-2023
Institut de Génétique Moléculaire de Montpellier
2023
Centre National de la Recherche Scientifique
2023
Université de Montpellier
2023
Howard Hughes Medical Institute
2013-2016
Yale University
2013-2016
University of New Haven
2016
Estación Experimental del Zaidín
2011-2014
Consejo Superior de Investigaciones Científicas
2010-2014
Long non-coding RNAs (lncRNAs) are key regulatory molecules, but unlike with other RNAs, the direct link between their tertiary structure motifs and function has proven elusive. Here we report structural functional studies of human maternally expressed gene 3 (MEG3), a tumor suppressor lncRNA that modulates p53 response. We found that, in an evolutionary conserved region MEG3, two distal interact by base complementarity to form alternative, mutually exclusive pseudoknot structures ("kissing...
LincRNA-p21 is a long intergenic non-coding RNA (lincRNA) involved in the p53-mediated stress response. We sequenced human lincRNA-p21 (hLincRNA-p21) and found that it has single exon includes inverted repeat Alu elements (IRAlus). Sense antisense fold independently of one another into secondary structure conserved among primates. Moreover, structures formed by IRAlus are localization hLincRNA-p21 nucleus, where colocalizes with paraspeckles. Our results underscore importance for function during
Abstract Group II introns are ubiquitous self-splicing ribozymes and retrotransposable elements evolutionarily chemically related to the eukaryotic spliceosome, with potential applications as gene-editing tools. Recent biochemical structural data have captured intron in multiple conformations at different stages of catalysis. Here, we employ enzymatic assays, X-ray crystallography, molecular simulations resolve spatiotemporal location function conformational changes occurring between first...
Group II introns act as both large catalytic RNAs and mobile retroelements. They are found in organelle bacterial genomes spliced via a lariat intermediate, mechanism similar to that of spliceosomal introns. However, their distribution insertion patterns, particularly for group introns, suggest they function behave more like retroelements than RmInt1 is an efficient intron within the IS Rm2011-2 sequence symbiotic bacterium Sinorhizobium meliloti . This excised, vivo vitro , lariats....
Despite hundreds of RNA modifications described to date, only editing results in a change the nucleotide sequence molecules compared genome. In mammals, two kinds have been so far, adenosine inosine (A-to-I) and cytidine uridine (C-to-U) editing. Recent improvements sequencing technologies led discovery continuously growing number sites. These methods are powerful but not error-free, making routine validation newly-described sites necessary. During one these validations on DDX58 mRNA, along...
Long noncoding RNAs (lncRNAs) are recently discovered transcripts that regulate vital cellular processes, such as differentiation and DNA replication, crucially connected to diseases. Although the 3D structures of lncRNAs key determinants their function, unprecedented molecular complexity has so far precluded structural characterization at high resolution. It is thus paramount develop novel approaches for biochemical biophysical these challenging targets. Here, we present a protocol...
Group II introns are self-splicing, retrotransposable ribozymes that contribute to gene expression and evolution in most organisms. The ongoing identification of new group recent bioinformatic analyses have suggested there novel lineages, which include the IIE IIF introns. Because function biochemical activity never been experimentally tested because these appear features distinguish them from other introns, we set out determine if they were indeed catalytically active RNA molecules. To this...
Abstract Background Group II intron splicing proceeds through two sequential transesterification reactions in which the 5' and 3'-exons are joined together lariat is released. The intron-encoded protein (IEP) assists of vivo remains bound to excised RNA a ribonucleoprotein particle (RNP) that promotes mobility. Exon recognition occurs base-pairing interactions between guide sequences on ribozyme domain dI known as EBS1 EBS2 stretches sequence IBS1 IBS2 exon, whereas 3' exon recognized...
Group II introns are catalytic RNAs that excised from their precursors in a protein-dependent manner vivo. Certain group can also react protein-independent under nonphysiological conditions vitro. The efficiency and fidelity of the splicing reaction is crucial, to guarantee correct formation expression protein-coding mRNA. RmInt1 an efficient mobile intron found within IS Rm2011-2 insertion sequence symbiotic bacterium Sinorhizobium meliloti . self-splices vitro, but this generates side...
Abstract While the regulatory role of lincRNAs is becoming increasingly appreciated, functional RNA structures within these molecules not well understood. We have embarked on a study to understand folding lincRNA molecules, and identify structural modules large transcripts. Using approaches promote native we been purifying lincRNAs, mapping their with chemical probes, then using this information construct maps architecture. This work complemented by phylogenetic genetic for exploring...