A5007909912- RNA and protein synthesis mechanisms
- RNA modifications and cancer
- RNA Research and Splicing
- interferon and immune responses
- Advanced biosensing and bioanalysis techniques
- Bacteriophages and microbial interactions
- Viral Infections and Immunology Research
- DNA and Nucleic Acid Chemistry
- Immune Response and Inflammation
- Hepatitis C virus research
- Cancer-related molecular mechanisms research
- Genomics and Phylogenetic Studies
- Immune Cell Function and Interaction
- RNA regulation and disease
- Hepatitis B Virus Studies
- Chemical Synthesis and Analysis
- Influenza Virus Research Studies
- Monoclonal and Polyclonal Antibodies Research
- RNA Interference and Gene Delivery
- Cancer Research and Treatments
- Immunotherapy and Immune Responses
- Inflammasome and immune disorders
- Cytokine Signaling Pathways and Interactions
- Bacterial Genetics and Biotechnology
- Enzyme Structure and Function
Howard Hughes Medical Institute
2016-2025
Yale University
2016-2025
University of New Haven
2012-2023
University of Colorado Boulder
1990-2023
Whitney Museum of American Art
2003-2023
Vanderbilt-Ingram Cancer Center
2019
Vanderbilt University
2019
Institut thématique Génétique, génomique et bioinformatique
2012-2015
Florida State University
2011
Charoenkrung Pracharak Hospital
2007
Human melanoma cells can be reprogrammed to terminally differentiate and irreversibly lose proliferative capacity by appropriate pharmacological manipulation. Subtraction hybridization identified differentiation-associated gene-5 ( mda-5 ) as a gene induced during differentiation, cancer reversion, programmed cell death (apoptosis). This contains both caspase recruitment domain putative DExH group RNA helicase domains. Atypical motifs of MDA-5 deviate from consensus sequences but are well...
Group II introns are self-splicing ribozymes that catalyze their own excision from precursor transcripts and insertion into new genetic locations. Here we report the crystal structure of an intact, self-spliced group intron Oceanobacillus iheyensis at 3.1 angstrom resolution. An extensive network tertiary interactions facilitates ordered packing subdomains around a ribozyme core includes catalytic domain V. The bulge V adopts unusual helical is located adjacent to major groove triple helix...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTDNA photocleavage by phenanthrenequinone diimine complexes of rhodium(III): shape-selective recognition and reactionAyesha Sitlani, Eric C. Long, Anna M. Pyle, Jacqueline K. BartonCite this: J. Am. Chem. Soc. 1992, 114, 7, 2303–2312Publication Date (Print):March 1, 1992Publication History Published online1 May 2002Published inissue 1 March 1992https://pubs.acs.org/doi/10.1021/ja00033a003https://doi.org/10.1021/ja00033a003research-articleACS...
RNA molecules adopt specific three-dimensional structures critical to their function. Many essential metabolic processes, including protein synthesis and splicing, are carried out by with elaborate tertiary (e.g. 3QIQ, right). Indeed, the ribosome self-splicing introns complex machines. But even coding regions in messenger RNAs viral flanked highly structured untranslated regions, which provide regulatory information necessary for gene expression.RNA structure is defined as arrangement of...
NS3, an essential helicase for replication of hepatitis C virus, is a model enzyme investigating function. Using single-molecule fluorescence analysis, we showed that NS3 unwinds DNA in discrete steps about three base pairs (bp). Dwell time analysis indicated hidden are required before 3-bp step taken. Taking into account the available structural data, propose spring-loaded mechanism which several one nucleotide per adenosine triphosphate molecule accumulate tension on protein-DNA complex,...
Significance Rhinovirus is the most frequent cause of common cold, as well one important causes asthma exacerbations. Most rhinovirus strains replicate better at cooler temperatures found in nasal cavity than lung temperature, but underlying mechanisms are not known. Using a mouse-adapted virus, we that airway epithelial cells supporting replication initiate more robust antiviral defense response through RIG-I–like receptor (RLR)–dependent interferon secretion and enhanced responsiveness...
There are currently limited Food and Drug Administration (FDA)-approved drugs vaccines for the treatment or prevention of Coronavirus Disease 2019 (COVID-19). Enhanced understanding Severe Acute Respiratory Syndrome 2 (SARS-CoV-2) infection pathogenesis is critical development therapeutics. To provide insight into viral replication, cell tropism, host–viral interactions SARS-CoV-2, we performed single-cell (sc) RNA sequencing (RNA-seq) experimentally infected human bronchial epithelial cells...
Abstract SARS-CoV-2 remdesivir resistance mutations have been generated in vitro but not reported patients receiving treatment with the antiviral agent. We present a case of an immunocompromised patient acquired B-cell deficiency who developed indolent, protracted course infection. Remdesivir therapy alleviated symptoms and produced transient virologic response, her was complicated by recrudescence high-grade viral shedding. Whole genome sequencing identified mutation, E802D, nsp12...
All aspects of cellular RNA metabolism and the replication many viruses require DExH/D proteins that manipulate in a manner requires nucleoside triphosphates. Although have been shown to unwind purified duplexes, most molecules environment are complexed with proteins. It has therefore speculated may also affect RNA-protein interactions. We demonstrate DExH protein NPH-II from vaccinia virus can displace U1A an active adenosine triphosphate–dependent fashion. increases rate dissociation by...
A consensus classification and nomenclature are defined for RNA backbone structure using all of the torsion angles. By a several independent analysis methods, 46 discrete conformers identified as suitably clustered in quality-filtered, multidimensional dihedral angle distribution. Most these represent identifiable features or roles within structures. The given two-character names that reflect seven-angle delta epsilon zeta alpha beta gamma combinations empirically found favorable...
Group II introns are self-splicing ribozymes that share a reaction mechanism and common ancestor with the eukaryotic spliceosome, thereby providing model system for understanding chemistry of pre-mRNA splicing. Here we report 14 crystal structures group intron at different stages catalysis. We provide detailed first step splicing, describe reversible conformational change between second steps present ligand-free structure after splicing in an active state corresponds to retrotransposable...