A5068789538- Clostridium difficile and Clostridium perfringens research
- Microscopic Colitis
- Gut microbiota and health
- Gastrointestinal motility and disorders
- Helicobacter pylori-related gastroenterology studies
- Microbial Metabolic Engineering and Bioproduction
- Vestibular and auditory disorders
- Enzyme function and inhibition
- Neuroscience and Neuropharmacology Research
- Hydrocarbon exploration and reservoir analysis
- Nosocomial Infections in ICU
- biodegradable polymer synthesis and properties
- Bacterial Genetics and Biotechnology
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Metabolomics and Mass Spectrometry Studies
- Fungal and yeast genetics research
- Respiratory Support and Mechanisms
- Lipid Membrane Structure and Behavior
- Hearing, Cochlea, Tinnitus, Genetics
University of Michigan
2017-2025
California Institute of Technology
2014-2017
University of Missouri–Kansas City
2010-2013
Inhaled oxygen alters the microbiota of mice and humans, which may contribute to oxygen-induced lung injury.
Significance Quiescent bacteria are intrinsically resistant to antibiotics and the host immune response. A conserved bacterial starvation survival response is consumption of ATP make an inorganic polymer, polyphosphate (polyP), which then forms granule superstructures. PolyP granules occur in all three domains life, yet how why cells form these structures poorly understood. Through high-resolution spatiotemporal characterization de novo genesis, we find that polyP synthesis required for...
Abstract Background Clostridioides difficile is a toxin-producing bacteria that the most common cause of nosocomial diarrhea. The CDC estimates there are around 500,000 infections caused by C. each year and has declared an urgent public health threat . production toxin thought to be primary driver disease pathogenesis. To understand relationship between severity, we analyzed correlation clinical histopathologic scores amount using murine model. Real Time Cellular Analysis utilizing Aligent...
The organization of lipids within biological membranes is poorly understood. Some studies have suggested group into microdomains cells, but the evidence remains controversial due to non-native imaging techniques. A recently developed NanoSIMS technique indicated that sphingolipids human fibroblast cells. We extended this approach study localization hopanoid in bacterial cells by developing a stable isotope labeling method directly detect subcellular specific bacteria with ca. 60 nm...
The incidence of C. difficile infection (CDI) has increased significantly among patients with IBD, independently antibiotic use, yet the relationship between IBD and risk for CDI remains to be understood. Our study sought describe utilize an antibiotic-independent mouse model specifically explore IBD-associated gut susceptibility colonization development.
Clostridioides difficile infection (CDI) can result in severe disease and death, with no accurate models that allow for early prediction of adverse outcomes. To address this need, we sought to develop serum-based biomarker predict CDI We prospectively collected sera ≤48 h after diagnosis two cohorts. Biomarkers were measured a custom multiplex bead array assay. Patients classified using IDSA severity criteria the development disease-related complications (DRCs), which defined as ICU...
The model filamentous fungus Neurospora crassa has been studied for over fifty years and many temperature-sensitive mutants have generated. While most of these mapped genetically, remain anonymous. mutation in the N. lethal mutant un-7 was identified by a complementation based approach as being open reading frame designated NCU00651 on linkage group I. Other mutations this gene that lead to morphological phenotype called png-1. underlying result serine phenylalanine change at position 273 an...
Clostridioides (formerly Clostridium ) difficile is the most common cause of hospital-acquired infection, and advanced age a risk factor for C. infection. Disruption intestinal microbiota immune responses contribute to host susceptibility severity However, specific impact aging on during infection remains be well described.
ABSTRACT Clostridioides (formerly Clostridium ) difficile is the most common cause of hospital-acquired infection, and advanced age a risk factor for C. infection. Disruption intestinal microbiota immune responses contribute to host susceptibility severity However, impact aging on cellular response associated with infection in setting remains be well described. This study explores effect as disease severity. Young adult mice (2-3 months old) aged (22-28 were rendered susceptible cefoperazone...
Abstract Background C. difficile infection is more common and severe in people over 65-years old. Understanding the pathogenesis within triangle of host, pathogen, microbiota aged population will help decrease disease severity. Methods Young (3-month-old) (22 to 25-month-old) male female C57BL/6 mice were treated with 0.5g/L cefoperazone for ten days render them susceptible infection. Antibiotic-treated challenged 103 104 spores via oral gavage. Three strains selected challenge. These vary...
Abstract Background Clostridium difficile infection (CDI) can result in severe disease and death. We are currently unable to identify patients at risk for developing adverse outcomes. previously showed multiple inflammatory mediators were associated with severity Here, we set out validate these findings a murine model of CDI. Methods CDI was diagnosed by the clinical microbiology laboratory. Sera collected ≤48 hours after diagnosis from pilot (October 2010–November 2012) validation...
Abstract Clostridium difficile (C. difficile) is a spore-forming bacterium that produces enterotoxins damage the intestinal epithelium. The incidence of C. infection (CDI) increased significantly over last two decades, particularly among those ≥65 years age. objective this study was to determine role age in immune responses mice, utilizing strains varying virulence. We have shown mice infected with strain VPI 10463 (VPI) exhibit more severe disease compared 630. Thus, examine an age-related...
ABSTRACT Clostridioides difficile has emerged as a noteworthy pathogen in patients with inflammatory bowel disease (IBD). Concurrent IBD and CDI is associated increased morbidity mortality compared to alone. alterations of the gut microbiota, an important mediator colonization resistance C. . Here, we describe utilize mouse model explore role intestinal inflammation susceptibility subsequent severity animals underlying IBD. Helicobacter hepaticus , normal member was used trigger distal...