A5025644300- Hepatitis C virus research
- Hepatitis B Virus Studies
- HIV Research and Treatment
- T-cell and Retrovirus Studies
- interferon and immune responses
- Liver Disease Diagnosis and Treatment
- RNA Research and Splicing
- Animal Disease Management and Epidemiology
- RNA modifications and cancer
- Chromosomal and Genetic Variations
- Virus-based gene therapy research
- Biochemical and Molecular Research
- Vector-Borne Animal Diseases
- Animal Virus Infections Studies
- DNA Repair Mechanisms
- CRISPR and Genetic Engineering
- RNA regulation and disease
- HIV/AIDS drug development and treatment
- RNA Interference and Gene Delivery
- SARS-CoV-2 and COVID-19 Research
- Viral Infections and Immunology Research
- COVID-19 Clinical Research Studies
- NF-κB Signaling Pathways
- Systemic Lupus Erythematosus Research
- RNA and protein synthesis mechanisms
Kumamoto University
2011-2023
National Institute of Infectious Diseases
2022-2023
Okayama University
2007-2013
Nagoya University
2012
University of Geneva
2005-2006
Kyoto University
1993-2005
Shionogi (Japan)
1993
Osaka Medical and Pharmaceutical University
1993
Kyoto Prefectural University of Medicine
1993
ABSTRACT DDX3, a DEAD-box RNA helicase, binds to the hepatitis C virus (HCV) core protein. However, role(s) of DDX3 in HCV replication is still not understood. Here we demonstrate that accumulation both genome-length (HCV-O, genotype 1b) and its replicon were significantly suppressed HuH-7-derived cells expressing short hairpin targeted by lentivirus vector transduction. As well, JFH1 (genotype 2a) release into culture supernatants knockdown after inoculation cell culture-generated HCVcc....
The microRNA miR-122 and DDX6/Rck/p54, a effector, have been implicated in hepatitis C virus (HCV) replication. In this study, we demonstrated for the first time that HCV-JFH1 infection disrupted processing (P)-body formation of effectors DDX6, Lsm1, Xrn1, PATL1, Ago2, but not decapping enzyme DCP2, dynamically redistributed these to HCV production factory around lipid droplets HuH-7-derived RSc cells. Notably, also stress granule components GTPase-activating protein (SH3 domain)-binding 1...
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has the largest RNA genome, approximately 30 kb, among viruses. The DDX DEAD box helicase is a multifunctional protein involved in all aspects of metabolism. Therefore, host helicases may regulate and maintain such large viral genome. In this study, I investigated potential role several cellular SARS-CoV-2 infection. Notably, DDX21 knockdown markedly accumulated intracellular production, as well infectivity SARS-CoV-2, indicating...
Integration of a DNA copy the viral RNA genome is crucial step in life cycle human immunodeficiency virus type 1 (HIV-1) and other retroviruses. While virally encoded integrase key to this process, cellular factors yet be characterized are suspected participate its completion. damage sensors such as ATM (ataxia-telangiectasia mutated), ATR (ATM- Rad3-related), DNA-PK (DNA-dependent protein kinase), PARP-1 [poly(ADP-ribose) polymerase 1] play central roles responses various forms injury could...
Background Recently, lipid droplets have been found to be involved in an important cytoplasmic organelle for hepatitis C virus (HCV) production. However, the mechanisms of HCV assembly, budding, and release remain poorly understood. Retroviruses some other enveloped viruses require endosomal sorting complex required transport (ESCRT) components their associated proteins budding process. Methodology/Principal Findings To determine whether or not ESCRT system is needed production, we examined...
Cellular responses to DNA damage are crucial for maintaining genome integrity, virus infection, and preventing the development of cancer. Hepatitis C (HCV) infection expression HCV nonstructural protein NS3 core have been proposed as factors involved in induction double-stranded breaks enhancement mutation frequency cellular genes. Since sensors, such ataxia-telangiectasia mutated kinase (ATM), ATM- Rad3-related (ATR), poly(ADP-ribose) polymerase 1 (PARP-1), checkpoint 2 (Chk2), play central...
Abstract Integration of human immunodeficiency virus type 1 (HIV-1) into the host genome is catalyzed by viral integrase (IN) and preferentially occurs within transcriptionally active genes. During early phase HIV-1 infection, incoming preintegration complex (PIC) recruits interactor (INI1)/hSNF5, a chromatin remodeling factor which directly binds to IN. The impact this event on replication so far unknown, although it has been hypothesized that could tether genes, thus contributing bias HIV...
Abstract Recently, we reported that β-carotene, vitamin D 2 , and linoleic acid inhibited hepatitis C virus (HCV) RNA replication in hepatoma cells. Interestingly, the course of study, found antioxidant E negated anti-HCV activities these nutrients. These results suggest oxidative stress caused by three nutrients is involved their activities. However, molecular mechanism which induces status remains unknown. Oxidative also known to activate extracellular signal-regulated kinase (ERK)....
Pol283-8-specific, HLA-B*51:01-restricted, cytotoxic T cells (CTLs) play a critical role in the long-term control of HIV-1 infection. However, these CTLs select for reverse transcriptase (RT) I135X escape mutation, which may be accumulating circulating sequences. We investigated selection mutation by specific same epitope but restricted HLA-B*52:01. found that HLA-B*52:01-restricted were elicited predominantly chronically HIV-1-infected individuals. These had strong ability to suppress...
Arsenic trioxide (ATO), a therapeutic reagent used for the treatment of acute promyelocytic leukemia, has recently been reported to increase human immunodeficiency virus type 1 infectivity. However, in this study, we have demonstrated that replication genome-length hepatitis C (HCV) RNA (O strain genotype 1b) was notably inhibited by ATO at submicromolar concentrations without cell toxicity. HCV-JFH1 (genotype 2a) and release core protein into culture supernatants were also after HCV...
Ribavirin (RBV) is often used in conjunction with interferon-based therapy for patients chronic hepatitis C. There a drastic difference the anti-hepatitis C virus (HCV) activity of RBV between HuH-7-derived assay system, OR6, possessing RBV-resistant phenotype (50% effective concentration [EC50 ]: >100 µM) and recently discovered Li23-derived ORL8, RBV-sensitive (EC50 : 8 µM; clinically achievable concentration). This because anti-HCV was mediated by inhibition inosine monophosphate...