A5011627576- Vibrio bacteria research studies
- Aquaculture disease management and microbiota
- Identification and Quantification in Food
- Salmonella and Campylobacter epidemiology
- Antibiotic Resistance in Bacteria
- Microbial infections and disease research
- Listeria monocytogenes in Food Safety
- Yersinia bacterium, plague, ectoparasites research
- Biosensors and Analytical Detection
- Chemical Synthesis and Analysis
- Healthcare Regulation
- Chemotherapy-related skin toxicity
- Cancer and Skin Lesions
- Bacterial Identification and Susceptibility Testing
- Cutaneous lymphoproliferative disorders research
- Bacterial biofilms and quorum sensing
- Zoonotic diseases and public health
- Dental materials and restorations
- Bone Tissue Engineering Materials
- Water Quality and Pollution Assessment
- S100 Proteins and Annexins
- Environmental DNA in Biodiversity Studies
- Water Quality and Resources Studies
- Mycobacterium research and diagnosis
- Bacillus and Francisella bacterial research
Universidade Federal de Pernambuco
2021-2024
Centro de Tecnologias Estratégicas do Nordeste
2022
Fundação Oswaldo Cruz
2012-2020
Acinetobacter baumannii is an opportunistic bacterial pathogen infecting immunocompromised patients and has gained attention worldwide due to its increased antimicrobial resistance. Here, we report a comparative whole-genome sequencing analysis coupled with assessment of antibiotic resistance 46 strains (45 A. plus one nosocomialis) originated from five hospitals the city Recife, Brazil, between 2010 2014. An average 3,809 genes were identified per genome, although only 2,006 single copy...
Aeromonas spp. are gram-negative bacteria that can cause a variety of infections in both humans and animals play controversial role diarrhea outbreaks. Our aim was to identify clinical environmental isolates associated with cholera outbreak northeast county Brazil at the species level. We also aimed determine genetic structure bacterial population virulence potential isolates.Analysis based on concatenated sequences 16S rRNA gyrB genes suggested classification 119 studied into following...
This work aimed to assess pathogenic potential and clonal relatedness of Aeromonas sp. Vibrio cholerae isolates recovered during a diarrhea outbreak in Brazil. Clinical environmental were investigated for the presence known genes was assessed by intergenic spacer region (ISR) 16S-23S amplification. Four (lip, exu, gcat, flaA/B) found at high overall frequency both clinical although lip gene specifically absent from selected species. A fifth gene, aerA, rarely A. caviae, most abundant The ISR...
Here, we report the isolation of 31 Acinetobacter baumannii strains producing OXA-253 in a single large Brazilian city. These belonged to five different sequence types (STs), including 4 STs not previously associated with blaOXA-253 In all strains, gene was located plasmid within genetic environment similar what found Brazil and Italy. The reported data emphasize successful transmission through area tendency for this resistance determinant remain A. population.
Introduction The number of reports intestinal infections caused by Aeromonas spp. has increased significantly in recent years. In most clinical laboratories, identification these bacteria is carried out general phenotypic tests that sometimes do not accurately differentiate and Vibrio. Methods A duplex-polymerase chain reaction (PCR) was developed directed to 2 targets identifying pathogenic humans. Results duplex-PCR results were reproducible specific for Conclusions This method will allow...
Plague caused by Yersinia pestis is one of the infectious diseases subject to International Health Regulations (IHR). Permanent monitoring focal plague areas mandatory in order enable prompt control measures prevent spread disease. Therefore, availability efficient diagnosis tests paramount importance. Here, we describe a loop-mediated isothermal amplification (LAMP)-based procedure for rapid Y. detection. We constructed set LAMP primers, which were used assays establish reaction conditions...
Rapid identification and characterization of Listeria monocytogenes are required for the food industry, epidemiological studies, disease prevention control. However, typing procedures labor-intensive time-consuming, they require technical expertise, a panel sera reference culture strains or sophisticated expensive equipment. To improve upon traditional diagnostic methods L. we developed evaluated an efficient procedure specific major pathogenic serotypes species based on loop-mediated...
Aeromonads are mainly opportunistic pathogens; however, many species emerging as important human pathogens. Therefore, monitoring these bacteria and their accurate characterization of its is highly important. Aeromonas Aer593 strain was recovered from a diarrhoea outbreak did not group with any previously described by housekeeping gene sequencing. To clarify the taxonomic position Aer593, genome sequenced analysed multilocus phylogenetic analysis (MLPA), in silico DNA-DNA hybridization...
Titanium is usually used in the manufacturing of metal implants due to its biocompatibility and high resistance corrosion. A structural functional connection between living bone surface implant, a process called osseointegration, mandatory for avoiding prolonged healing, infections, tissue loss. Therefore, osseointegration crucial success implantation procedure. Osseointegration mediated by bone-matrix progenitor cells' proteins, named integrins. In this study, we an silico approach assemble...
Life-threatening bacterial infections are a major concern in health care services worldwide. This retrospective study aimed to demonstrate genetic and biochemical diversity isolates of Acinetobacter baumannii Pseudomonas aeruginosa from public hospital Brazil. A total 63 collected different sites infection sectors were characterized, their susceptibility profile antibiotics was assessed for 18 drugs belonging 8 antimicrobial categories using the automated BACTEC system. Genetic multiple...
After the worldwide cholera epidemic in 1993, permanent environmental monitoring of hydrographic basins was established Pernambuco, Brazil, where is endemic. a quiescent period, 4 rfb N (serogroup O1) positive water samples that were culture negative detected by multiplex single‐tube nested PCR (MSTNPCR); 2 these also ctx A (cholera toxin) positive. From May to June 2012, 30 V. cholerae O1 isolates obtained culturing samples. These analyzed for presence virulence genes PCR, intergenic spacer...
Background: In Brazil, listeriosis is not a notifiable disease; thus, the incidence of Brazilian cases remains unknown. Listeria monocytogenes always included in automated systems, and its detection depends on high skill level microbiology laboratory professionals. This paper describes characteristics L. isolates fortuitously obtained from an endocarditis case Recife, PE, Brazil. Methods: Six bacterial six blood cultures 28-year-old male bearing prosthetic mitral heart valve were analyzed by...