A5021675226- Yersinia bacterium, plague, ectoparasites research
- Vibrio bacteria research studies
- Salmonella and Campylobacter epidemiology
- Zoonotic diseases and public health
- Aquaculture disease management and microbiota
- Vector-borne infectious diseases
- Bacterial biofilms and quorum sensing
- Antimicrobial Resistance in Staphylococcus
- Antibiotic Resistance in Bacteria
- Bacterial Identification and Susceptibility Testing
- Bacillus and Francisella bacterial research
- Mosquito-borne diseases and control
- Identification and Quantification in Food
- Mycobacterium research and diagnosis
- Bee Products Chemical Analysis
- Child Nutrition and Water Access
- Pharmacological Effects of Natural Compounds
- Listeria monocytogenes in Food Safety
- Food Safety and Hygiene
- Enterobacteriaceae and Cronobacter Research
- Tuberculosis Research and Epidemiology
- Plant-based Medicinal Research
- Bacteriophages and microbial interactions
- Fecal contamination and water quality
- Research on Leishmaniasis Studies
Fundação Oswaldo Cruz
2014-2024
ORCID
2021
Polytechnic Institute of Coimbra
2020
Universidade Federal Rural de Pernambuco
2009
Faculdade de Agudos
1999-2004
Universidade Federal de Pernambuco
1991-2001
Universidade Federal de São Paulo
2001
Hospital das Clínicas da Universidade Federal de Pernambuco
1994
The natural history of lymphatic disease in human filariasis remains unclear, but recurrent episodes acute lymphangitis are believed to constitute a major risk factor for the development chronic lymphoedema and elephantiasis. Prospective analysis 600 patients referred clinic Centro de Pesquisas Aggeu Magalhães/FIOCRUZ Recife, Brazil, indicated that 2 distinct syndromes accompanied by occur residents this filariasis-endemic area. One syndrome, which we call filarial (AFL), is caused death...
Acinetobacter baumannii is an opportunistic bacterial pathogen infecting immunocompromised patients and has gained attention worldwide due to its increased antimicrobial resistance. Here, we report a comparative whole-genome sequencing analysis coupled with assessment of antibiotic resistance 46 strains (45 A. plus one nosocomialis) originated from five hospitals the city Recife, Brazil, between 2010 2014. An average 3,809 genes were identified per genome, although only 2,006 single copy...
Aeromonas spp. are gram-negative bacteria that can cause a variety of infections in both humans and animals play controversial role diarrhea outbreaks. Our aim was to identify clinical environmental isolates associated with cholera outbreak northeast county Brazil at the species level. We also aimed determine genetic structure bacterial population virulence potential isolates.Analysis based on concatenated sequences 16S rRNA gyrB genes suggested classification 119 studied into following...
Acinetobacter baumannii is one of the most frequent Gram-negative opportunistic pathogens associated with hospital-acquired infection worldwide. We briefly describe A. isolates that were recovered from surrounding ICU bed surfaces, exhibiting multidrug resistance phenotype and belonging to some widely spread clonal complexes clinical isolates.
We have developed a procedure for the rapid diagnosis of plague that also allows identification prominent virulence markers Y. pestis strains. This is based upon use single polymerase chain reaction with multiple pairs primers directed at genes present in three plasmids as well chromosomal pathogenicity island bacterium. The technique allowed discrimination strains which lacked one or more known pathogenic loci, using template total DNA obtained from bacterial cultures and simulated blood...
To evaluate the utility of random amplification polymorphic DNA (RAPD) technique for routine practice in public health laboratories epidemiological studies Vibrio cholerae O1 isolates.Seventy-nine strains were examined by PCR toxin genes (ctx A, zot and ace), virulence-associated (tcp A tox T) RAPD sequences. Except one strain (no. 1123) from Amazonas State, all analysed carried ctx zot, ace, tcp T. fingerprinting revealed variability but no correlation with serotype, biotype or geographical...
To examine the virulence factors and genetic relationship isolates of serogroup O3 Vibrio parahaemolyticus in outbreaks diarrhoea northeast region Brazil.Eighteen samples O3:K6 O3:KUT serotypes V. were analysed by multiplex polymerase chain reaction (m-PCR) for detection tl, tdh trh genes, random-amplified polymorphic DNA (RAPD) using two primers, amplification rDNA 16S-23S region. The gene tl was amplified all samples, 16 while none; generated only one profile; each RAPD primer produced...
Geographical differences in the prevalence of Helicobacter pylori genes and their association with disease severity have been identified. This study analyzes prevalences cagA gene alleles vacA H. pylori-associated gastroduodenal diseases isolates from Recife, PE, Brazil. Gastric biopsy 61 pylori-positive patients were submitted to DNA extraction amplification by polymerase chain reaction. Among patients, 21 suffered duodenal ulcer (DU) 40 gastritis (GT). The strains harbouring was higher DU...
Methicillin-resistant Staphylococcus aureus (MRSA) strains have been responsible for many nosocomial outbreaks. Within hospitals, colonized employees often act as reservoirs the spread of this organism. This study collected clinical samples 91 patients admitted to intensive care unit (ICU), hemodialysis/nephrology service and surgical clinic, biological from nasal cavities 120 professionals working in those environments, a University Hospital Recife, State Pernambuco, Brazil. The main...
Journal Article Typing of human and bovine Staphylococcus aureus by RAPD‐PCR ribotyping‐PCR Get access M.S.V. Pereira, Pereira Universidade Federal de Pernambuco, Recife, PE, BrazilUniversidade da Paraíba, Joa˜o Pessoa, PB, Brazil Dr , DBM/CCEN, 58059–900 Pessoa (PB), (e‐mail: svieira@dbm.ufpb.br). Search for other works this author on: Oxford Academic Google Scholar N.C. Leal, Leal Centro Pesquisas Aggeu Magalha˜es – FIOCRUZ, T.C.A. M. Sobreira, Sobreira A.M.P. De Almeida, Almeida J.P....
During a plaque outbreak in the Borborema Plateau focus (Paraiba), bacteriological and serological studies were carried out material from 452 patients (48 positives), 1,938 rodents other small mammals (75 4,756 dogs (141 positives) 2,047 cats (57 obtained 41 counties (out of which, 21 produced positive samples). Twenty Yersinia pestis strains isolated 3 17 rodents, displayed biochemical reactions, virulence factors, antibiotic susceptibility animal experimental pathogenicity similar to those...
This work aimed to assess pathogenic potential and clonal relatedness of Aeromonas sp. Vibrio cholerae isolates recovered during a diarrhea outbreak in Brazil. Clinical environmental were investigated for the presence known genes was assessed by intergenic spacer region (ISR) 16S-23S amplification. Four (lip, exu, gcat, flaA/B) found at high overall frequency both clinical although lip gene specifically absent from selected species. A fifth gene, aerA, rarely A. caviae, most abundant The ISR...
One hundred seventy nine Vibrio cholerae non-O1/non-O139 strains from clinical and different environmental sources isolated in Brazil 1991 to 2000 were serogrouped screened for the presence of four virulence factors. The Random Amplification Polymorphic DNA (RAPD) technique was used evaluate genetic relatedness among strains. Fifty-four serogroups identified V. O26 most common (7.8%). PCR analysis three genes (ctxA, zot, ace) located CTX element one gene (tcpA) on VPI pathogenicity island...
Here, we report the isolation of 31 Acinetobacter baumannii strains producing OXA-253 in a single large Brazilian city. These belonged to five different sequence types (STs), including 4 STs not previously associated with blaOXA-253 In all strains, gene was located plasmid within genetic environment similar what found Brazil and Italy. The reported data emphasize successful transmission through area tendency for this resistance determinant remain A. population.
In an attempt to elucidate the virulence factors and pathogenic mechanisms of Providencia alcalifaciens, 36 isolates identified in 1994–1995 Recife city, Brazil were analysed by PCR investigate presence DNA sequences homologous genes described other invasive enterobacteria, as well their ability invade HeLa cells, plasmid profiles antibiotic resistance patterns. The genetic diversity was also RAPD-PCR. No observed with any P. alcalifaciens studied. Ten had no 26 harboured one-to-five...