A5051099625- Legume Nitrogen Fixing Symbiosis
- Plant nutrient uptake and metabolism
- Coastal wetland ecosystem dynamics
- Bacterial Genetics and Biotechnology
- Escherichia coli research studies
- Bacteriophages and microbial interactions
- Microbial Community Ecology and Physiology
- Evolution and Genetic Dynamics
- Antimicrobial Resistance in Staphylococcus
- Protist diversity and phylogeny
- Plant responses to water stress
- Soil Carbon and Nitrogen Dynamics
- Nematode management and characterization studies
- Microbial Metabolism and Applications
- Plant Stress Responses and Tolerance
- Bacterial biofilms and quorum sensing
- Plant Pathogenic Bacteria Studies
- Agronomic Practices and Intercropping Systems
- Marine and coastal ecosystems
Smith College
2015-2022
Michigan State University
1998-2013
University of Massachusetts Amherst
2006-2013
University of Massachusetts System
2012
Bacteriocins are a large and functionally diverse family of toxins found in all major lineages Bacteria. Colicins, those bacteriocins produced by Escherichia coli, serve as model system for investigations bacteriocin structure–function relationships, genetic organization, their ecological role evolutionary history. Colicin expression is often dependent on host regulatory pathways (such the SOS system), usually confined to times stress, results death producing cells. This study investigates...
Abstract Sinorhizobium meliloti is a microorganism commercially used in the production of e.g . Medicago sativa seed inocula. Many inocula are powder-based and includes drying step. Although S. survives well, quality reduced during this process. In study we determined survival desiccation commercial strains 102F84 102F85 as well model strain USDA1021. The 1021 was estimated nine weeks at 22% relative humidity. We found that after an initial rapid decline colony forming units, slowed to...
To identify physical and physiological conditions that affect the survival of Sinorhizobium meliloti USDA 1021 during desiccation.An assay was developed to study desiccation response S. over a range environmental conditions. We determined in relation (i) matrices media, (ii) growth phase, (iii) temperature, (iv) chloride sulfate availability.This indicates is enhanced: when cells were dried stationary with increasing drying temperature at an optimum 37 degrees C, increase sulfate, but not...
ABSTRACT Stachydrine, a betaine released by germinating alfalfa seeds, functions as an inducer of nodulation genes, catabolite, and osmoprotectant in Sinorhizobium meliloti . Two stachydrine-inducible genes were found S. 1021 mutation with Tn 5-luxAB promoter probe. Both mutant strains (S10 S11) formed effective root nodules, but neither grew on stachydrine the sole carbon nitrogen source. When grown absence or presence salt stress, S10 S11 took up [ 14 C]stachydrine well wild-type cells...
ABSTRACT Elucidating the relationship between ciliate communities in benthos and plankton is critical to understanding diversity marine systems. Although data for many lineages are sparse, at least some members of dominant clades Oligotrichia Choreotrichia can be found both benthos, latter either as cysts or active forms. In this study, we developed a molecular approach address ciliates those underlying same locations. Samples from sediments were compared across three sites along New England...
Bacteria engage in a never-ending arms race which they compete for limited resources and niche space. The outcome of this intense interaction is the evolution powerful arsenal biological weapons. Perhaps most studied these are colicins, plasmid-based toxins produced by active against Escherichia coli. present study was designed to explore molecular responses colicin-producing strain during serial transfer evolution. What evolutionary changes occur when colicins with no target present? Can...
The Rhizobiaceae are a bacterial family of enormous agricultural importance due to the ability its members fix atmospheric nitrogen in an intimate relationship with plants. Their survival as naturally occurring soil bacteria soils well popular seed inocula is affected directly by drought and salinity. Survival after desiccation presence NaCl enabled underlying genetic mechanisms model organism Sinorhizobium meliloti 1021. Since salt stress parallels loss water activity, identification...
Ever since the first observation of antagonism by microorganisms Ernest Duchesne (E. Duchesne, Contribution à l’étude de la concurrence vitale chez les microorganisms. Antagonism entre moisissures et microbes, These pour obtenir le grade docteur en medicine, Lyon, France, 1897), many scientists successfully identified and applied bacteriogenic bioactive compounds from soils to cure infection.
Many Staphylococcus and Pseudomonas species, such as aureus aeruginosa , are opportunistic human pathogens. However, species also known to produce bioactive compounds. Here, we report on the genome sequences of a isolate unknown origin that it inhibits.