A5082379165- Cellular Mechanics and Interactions
- CAR-T cell therapy research
- Microfluidic and Bio-sensing Technologies
- RNA Interference and Gene Delivery
- Nuclear Structure and Function
- Nanowire Synthesis and Applications
- 3D Printing in Biomedical Research
- Viral Infectious Diseases and Gene Expression in Insects
The Wallace H. Coulter Department of Biomedical Engineering
2019-2023
Georgia Institute of Technology
2019-2023
Cells respond to mechanical forces by deforming in accordance with viscoelastic solid behavior. Studies of microscale cell deformation observed high speed video microscopy have elucidated a new behavior which sufficiently rapid compression cells can lead transient volume loss and then recovery. This work has discovered that the resulting exchange between interior surrounding fluid be utilized for efficient, convective delivery large macromolecules (2000 kDa) interior. However, many...
Stiffness has been observed to decrease for many cancer cell types as their metastatic potential increases. Although mechanics and are related, the underlying molecular factors associated with these phenotypes remain unknown. Therefore, we have developed a workflow measure mechanical properties gene expression of single cells that is used generate large linked-datasets. The process combines atomic force microscopy individual multiplexed RT-qPCR analysis on same cells. Surprisingly, genes...
We developed a workflow to produce potent gene-edited CAR T cells that combines microfluidic transfection and lentiviral transduction using healthy donor- patient-derived cells. an understanding on how biomechanics of impact mechanoporation.