A5009156872- Nanoparticle-Based Drug Delivery
- Monoclonal and Polyclonal Antibodies Research
- Nanoplatforms for cancer theranostics
- Glycosylation and Glycoproteins Research
- Cancer Research and Treatments
- Protein purification and stability
- Immunotherapy and Immune Responses
- Radiopharmaceutical Chemistry and Applications
- Ferroptosis and cancer prognosis
- Dendrimers and Hyperbranched Polymers
- Lung Cancer Research Studies
- Pancreatic and Hepatic Oncology Research
- Chemotherapy-induced cardiotoxicity and mitigation
- Neutropenia and Cancer Infections
- Clostridium difficile and Clostridium perfringens research
- Advanced biosensing and bioanalysis techniques
- Inflammasome and immune disorders
- Cancer therapeutics and mechanisms
- RNA modifications and cancer
- Dental Implant Techniques and Outcomes
- Advanced Biosensing Techniques and Applications
- Receptor Mechanisms and Signaling
- Bone and Dental Protein Studies
- Bone Tissue Engineering Materials
- Lymphoma Diagnosis and Treatment
Kaohsiung Medical University
1997-2024
National Yang Ming Chiao Tung University
2020
Academia Sinica
2020
National Sun Yat-sen University
2020
Kaohsiung Medical University Chung-Ho Memorial Hospital
2020
Renmin Hospital of Wuhan University
2018
Wuhan University
2018
Since the five-year survival rate is less than 5%, pancreatic ductal adenocarcinoma (PDAC) remains 4th cause of cancer-related death.Although PDAC has been repeatedly researched in recent years, it still predicted to be second leading cancer death by year 2030.In our study, differentially expressed genes dataset GSE62452 were used construct a co-expression network WGCNA.The yellow module related grade was screened.Combined with and PPI network, 36 candidates screened.After regression...
Abstract Systemic injection of therapeutic antibodies may cause serious adverse effects due to on-target toxicity the antigens expressed in normal tissues. To improve targeting selectivity region disease sites, we developed protease-activated pro-antibodies by masking binding sites with inhibitory domains that can be removed proteases are highly at sites. The latency-associated peptide (LAP), C2b or CBa complement factor 2/B were linked, through a substrate matrix metalloproteinase-2...
One-step formulation of BsAb with PLD is a simple method to enhance tumor specificity, internalization and the anti-cancer activity.
Abstract Background PEGylated nanoparticles (PEG-NPs) are not effective for hematologic malignancies as they lack the enhanced permeability and retention effect (EPR effect). Tumor-targeted PEG-NPs can systemically track lymphoma actively internalize into cancer cells to enhance therapeutic efficacy. We generated an anti-PEG bispecific antibody (BsAb; mPEG × CD20) which was able simultaneously bind methoxy PEG on liposomes CD20 form multivalent αCD20-armed liposomes. This liposome crosslink...
During rheumatoid arthritis (RA) treatment, long-term injection of antitumor necrosis factor α antibodies (anti-TNFα Abs) may induce on-target toxicities, including severe infections (tuberculosis [TB] or septic arthritis) and malignancy. Here, we used an immunoglobulin G1 (IgG1) hinge as Ab lock to cover the TNFα-binding site Infliximab by linking it with matrix metalloproteinase (MMP) -2/9 substrate generate pro-Infliximab that can be specifically activated in RA region enhance selectivity...
Spatial hindrance-based pro-antibodies (pro-Abs) are engineered antibodies to reduce monoclonal antibodies' (mAbs) on-target toxicity using universal designed blocking segments that mask mAb antigen-binding sites through spatial hindrance. By linking protease substrates and linkers, these can be removed site-specifically. Although many types of have been developed, such as coiled-coil hinge-based Ab locks, the molecular structure pro-Ab, particularly region showing how fragment blocks mAb,...
Sensitive determination of the pharmacokinetics PEGylated molecules can accelerate process drug development. Here, we combined different anti-PEG Fab expressing 293T cells as capture (293T/3.3, 293T/6.3, and 293T/15–2b cells) with four detective antibodies (3.3, 6.3, 7A4, or 15–2b) to optimize an cell-based sandwich ELISA. Then, quantified free PEG (mPEG2K-NH2 mPEG5K-NH2) PEG-conjugated small (mPEG5K-biotin mPEG5K-NIR797), proteins (PegIntron Pegasys), nanoparticles (Liposomal-Doxorubicin...
Abstract Background Developing a universal strategy to improve the specificity and sensitivity of PEGylated nanoaparticles (PEG-NPs) for assisting in diagnosis tumors is important multimodality imaging. Here, we developed anti-methoxypolyethylene glycol (mPEG) bispecific antibody (BsAb; mPEG × HER2), which has dual human epidermal growth factor receptor 2 (HER2), with diverse array PEG-NPs confer nanoparticles HER2 stronger intensity. Result We used one-step formulation rapidly modify...
Sensitive quantification of the pharmacokinetics poly(ethylene glycol) (PEG) and PEGylated molecules is critical for drug development. Here, we developed a sensitive sandwich enzyme-linked immunosorbent assay (ELISA) PEG by tethering an anti-PEG antibody (AGP3) via tethers with different dimensions on surface 293T cells (293T/S-αPEG, short-type cells; 293T/L-αPEG, long-type 293T/SL-αPEG, hybrid-type cells) to improve binding capacity detection limit free molecules. The PEG-like...
The gut microbiota is reported to play an important role in carcinogenesis and the treatment of CRC. SW480 SW620 colon cancer cells integrated with infrared fluorescent proteins were injected into rectal submucosa nude mice. In subsequent 30 days, we observed tumor growth weekly using vivo imaging system. bacterial solution was infused anally mice perform transplant. Phosphate-buffered saline, Acinetobacter lwoffii, Bifidobacterium longum solutions individually. 16S ribosomal DNA (rDNA)...
Abstract Canakinumab is a fully human monoclonal antibody that specifically neutralizes interleukin (IL)-1β and has been approved by the US Food Drug Administration for treating different types of autoinflammatory disorders such as cryopyrin-associated periodic syndrome, tumor necrosis factor receptor-associated syndrome systemic juvenile idiopathic arthritis. However, long-term neutralization IL-1β may cause severe adverse events serious upper respiratory tract infections inflammation,...
Clinical nanoparticles were armed with TsAb through a one-step non-covalent modification to achieve the maximal specific killing against TAF-rich solid tumors.
Membrane antigens (mAgs) are important targets for the development of antibody (Ab) drugs. However, native mAgs not easily prepared, causing difficulties in acquiring functional Abs. In this study, we present a platform which human were expressed form on cell adjuvants made with membrane-bound cytokines that then used immunize syngeneic mice directly. The as immune stimulators to enhance specific Ab responses against desired mAgs. Then, mAgs-expressing xenogeneic cells characterization...
Ovarian cancer is highly metastatic, with a high frequency of relapse, and the most fatal gynecologic malignancy in women worldwide. It important to elevate drug susceptibility cytotoxicity ovarian cells, thereby eliminating resident cells for more effective therapeutic efficacy. Here, we developed bispecific antibody (BsAb; mPEG × HER2) that can easily provide HER2+ tumor tropism mPEGylated liposomal doxorubicin (PLD) further increase accumulation via receptor-mediated endocytosis, improve...
Additional file 1: Table S1. The BsAb-conjugation rate of αHER2/PEG-NPs. S2. Characterization BsAb/mPEG-NPs. Figure Immunogenicity humanized BsAbs. We cocultured dendritic cells differentiated from human PBMCs with autologous CD4+ T and stimulated control medium (represented as DC+T), PHA (as positive control), PEG×HER2, PEG×DNS, respectively, for 5 days. Then, we detected the proliferation by ATPlite assay. Bars, SD. CPM, counts per minute; PBMC, peripheral blood mononuclear cell; PHA,...
Abstract Background: Developing a universal strategy to improve the specificity and sensitivity of PEGylated nanoaparticles (PEG-NPs) for assisting in diagnosis tumors is important multimodality imaging. Here, we developed anti-methoxypolyethylene glycol (mPEG) bispecific antibody (BsAb; mPEG×HER2), which has dual mPEG human epidermal growth factor receptor 2 (HER2), with diverse array PEG-NPs confer nanoparticles HER2 stronger intensity. Result: We used one-step formulation rapidly modify...
Abstract Background Tumor-targeted nanoparticles hold great promise as new tools for therapy of liquid cancers. Furthermore, the therapeutic efficacy can be improved by enhancing cancer cellular internalization. Methods In this study, we developed a humanized bispecific antibody (BsAbs: CD20 Ab×mPEG scFv) which retains clinical anti-CD20 whole (Ofatumumab) and is fused with anti-mPEG single chain antibodies (scFv) that target systemic tumor cells. This combination achieves function...