A5085108174- Monoclonal and Polyclonal Antibodies Research
- Nanoparticle-Based Drug Delivery
- Protein purification and stability
- Glycosylation and Glycoproteins Research
- Cell Adhesion Molecules Research
- Nanoplatforms for cancer theranostics
- Immunotherapy and Immune Responses
- Cancer Immunotherapy and Biomarkers
- Rheumatoid Arthritis Research and Therapies
- Cancer Research and Treatments
- Robotics and Sensor-Based Localization
- Probiotics and Fermented Foods
- Synthesis and biological activity
- Colorectal Cancer Treatments and Studies
- SARS-CoV-2 and COVID-19 Research
- Radiopharmaceutical Chemistry and Applications
- Pharmacological Effects and Toxicity Studies
- RNA Interference and Gene Delivery
- Pancreatic function and diabetes
- Electroconvulsive Therapy Studies
- Inflammasome and immune disorders
- Advanced Vision and Imaging
- Pharmacology and Obesity Treatment
- IL-33, ST2, and ILC Pathways
- 3D Shape Modeling and Analysis
National Sun Yat-sen University
2017-2024
Kaohsiung Medical University
2020-2024
Academia Sinica
2020-2024
Kaohsiung Medical University Chung-Ho Memorial Hospital
2020-2024
National Kaohsiung University of Science and Technology
2022
National Yang Ming Chiao Tung University
2020
National Taiwan University Hospital
2009
During rheumatoid arthritis (RA) treatment, long-term injection of antitumor necrosis factor α antibodies (anti-TNFα Abs) may induce on-target toxicities, including severe infections (tuberculosis [TB] or septic arthritis) and malignancy. Here, we used an immunoglobulin G1 (IgG1) hinge as Ab lock to cover the TNFα-binding site Infliximab by linking it with matrix metalloproteinase (MMP) -2/9 substrate generate pro-Infliximab that can be specifically activated in RA region enhance selectivity...
Abstract Background PEGylated nanoparticles (PEG-NPs) are not effective for hematologic malignancies as they lack the enhanced permeability and retention effect (EPR effect). Tumor-targeted PEG-NPs can systemically track lymphoma actively internalize into cancer cells to enhance therapeutic efficacy. We generated an anti-PEG bispecific antibody (BsAb; mPEG × CD20) which was able simultaneously bind methoxy PEG on liposomes CD20 form multivalent αCD20-armed liposomes. This liposome crosslink...
Coronavirus 3C-like protease (3CLpro) is found in SARS-CoV-2 virus, which causes COVID-19. 3CLpro controls virus replication and a major target for target-based antiviral discovery. As reported by Pfizer, Nirmatrelvir (PF-07321332) competitive protein inhibitor clinical candidate orally delivered medication. However, the binding mechanisms between complex structures remain unknown. This study incorporated ligand Gaussian accelerated molecular dynamics, one-dimensional two-dimensional...
Sensitive quantification of the pharmacokinetics poly(ethylene glycol) (PEG) and PEGylated molecules is critical for drug development. Here, we developed a sensitive sandwich enzyme-linked immunosorbent assay (ELISA) PEG by tethering an anti-PEG antibody (AGP3) via tethers with different dimensions on surface 293T cells (293T/S-αPEG, short-type cells; 293T/L-αPEG, long-type 293T/SL-αPEG, hybrid-type cells) to improve binding capacity detection limit free molecules. The PEG-like...
Abstract Background CTLA4Ig is a dimeric fusion protein of the extracellular domain cytotoxic T-lymphocyte 4 (CTLA4) and an Fc (Ig) fragment human IgG 1 that approved for treating rheumatoid arthritis. However, may induce adverse effects. Developing lesion-selective variant improve safety while maintaining efficacy treatment. Methods We linked albumin to N-terminus (termed Alb-CTLA4Ig) via substrate sequence matrix metalloproteinase (MMP). The binding activities biological Alb-CTLA4Ig before...
Abstract Canakinumab is a fully human monoclonal antibody that specifically neutralizes interleukin (IL)-1β and has been approved by the US Food Drug Administration for treating different types of autoinflammatory disorders such as cryopyrin-associated periodic syndrome, tumor necrosis factor receptor-associated syndrome systemic juvenile idiopathic arthritis. However, long-term neutralization IL-1β may cause severe adverse events serious upper respiratory tract infections inflammation,...
Membrane antigens (mAgs) are important targets for the development of antibody (Ab) drugs. However, native mAgs not easily prepared, causing difficulties in acquiring functional Abs. In this study, we present a platform which human were expressed form on cell adjuvants made with membrane-bound cytokines that then used immunize syngeneic mice directly. The as immune stimulators to enhance specific Ab responses against desired mAgs. Then, mAgs-expressing xenogeneic cells characterization...
Ovarian cancer is highly metastatic, with a high frequency of relapse, and the most fatal gynecologic malignancy in women worldwide. It important to elevate drug susceptibility cytotoxicity ovarian cells, thereby eliminating resident cells for more effective therapeutic efficacy. Here, we developed bispecific antibody (BsAb; mPEG × HER2) that can easily provide HER2+ tumor tropism mPEGylated liposomal doxorubicin (PLD) further increase accumulation via receptor-mediated endocytosis, improve...
The pro-Ab blocks the Ag binding site using an Ab lock. We designed a method which uses structure-based computational simulation (MSCS) to predict cover rate of locks with various linkers and select suitable linker for each Ab.
Abstract Background Humanization of mouse monoclonal antibodies (mAbs) is crucial for reducing their immunogenicity in humans. However, humanized mAbs often lose binding affinities. Therefore, an silico humanization method that can prevent the loss affinity needed. Methods We developed V(D)J recombination platform which we used human germline gene sequences to design five candidates anti-tumor necrosis factor (TNF)-α (C1–C5) by using different templates. The were subjected molecular dynamics...
Abstract Glycosidic switch liposome (GSL) technology uses a reversible glucuronide ester to efficiently encapsulate and stably retain potent anticancer drugs in liposomes. Parental drug is generated by enzymatic hydrolysis of the lysosomes target cells. Here we investigated if bispecific molecules simultaneously targeting polyethylene glycol (PEG) on GSL an internalizing tumor antigen could increase uptake into cancer cells enhance activity. A PEG engager (mPEG×EphA2) was fusing humanized...
In recent years, 3D models have been widely used in the virtual/augmented reality industry. The traditional way of constructing for real-world objects remains expensive and time-consuming. With rapid development graphics processors, many approaches based on deep learning proposed to reduce time economic cost generation object models. However, quality generated leaves considerable room improvement. Accordingly, we designed implemented a voxel generator called VoxGen, autoencoder framework. It...
Abstract Background Tumor-targeted nanoparticles hold great promise as new tools for therapy of liquid cancers. Furthermore, the therapeutic efficacy can be improved by enhancing cancer cellular internalization. Methods In this study, we developed a humanized bispecific antibody (BsAbs: CD20 Ab×mPEG scFv) which retains clinical anti-CD20 whole (Ofatumumab) and is fused with anti-mPEG single chain antibodies (scFv) that target systemic tumor cells. This combination achieves function...
Abstract Background: The on-target toxicity of monoclonal antibodies (Abs) is mainly due to the fact that Abs cannot distinguish target antigens (Ags) expressed in disease regions from those normal tissues during systemic administration. In order overcome this issue, we “copied” an autologous Ab hinge as “Ab lock” and “pasted” it on binding site by connecting a protease substrate linker between generate pro-Ab, which can be specifically activated region enhance selectivity reduce side...
Correction for ‘Development of a structure-based computational simulation to optimize the blocking efficacy pro-antibodies’ by Bo-Cheng Huang <italic>et al.</italic>, <italic>Chem. Sci.</italic>, 2021, DOI: 10.1039/D1SC01748A.