A5018913848- Liver Disease Diagnosis and Treatment
- Diet, Metabolism, and Disease
- Lipid metabolism and biosynthesis
- Diet and metabolism studies
- Obesity, Physical Activity, Diet
- Dietary Effects on Health
- Circadian rhythm and melatonin
- Nutritional Studies and Diet
- Behavioral Health and Interventions
- Sleep and related disorders
- Cancer, Hypoxia, and Metabolism
- Metabolomics and Mass Spectrometry Studies
- Organ Transplantation Techniques and Outcomes
- Health Promotion and Cardiovascular Prevention
- Obesity and Health Practices
- Thermoregulation and physiological responses
- Cancer, Lipids, and Metabolism
Oxford Centre for Diabetes, Endocrinology and Metabolism
2016-2020
University of Oxford
2016-2020
University of Surrey
2014-2019
Churchill Hospital
2016-2018
John Radcliffe Hospital
2016
ABSTRACT Conflicting evidence exists as to whether there are differences between males and females in circadian timing. The aim of the current study was assess sex present regulation melatonin cortisol plasma urine matrices during a constant routine protocol. Thirty-two healthy individuals (16 taking oral contraceptive pill (OCP)), aged 23.8 ± 3.7 (mean SD) years, participated. Blood (hourly) (4-hourly) samples were collected for measurement cortisol, urinary 6-sulfatoxymelatonin (aMT6s)...
Human primary hepatocytes are the gold standard for investigating lipid metabolism in nonalcoholic fatty liver disease (NAFLD); however, due to limitations including availability and donor variability, hepatoma cell lines Huh7 HepG2 commonly used. Culturing these human serum (HS) has been reported improve functionality; direct comparison of acid (FA) response culturing HS is lacking. The aim this study was compare FA between cells different sera. Both were grown media containing 11 mmol/L...
Objective Increased hepatic de novo lipogenesis (DNL) is suggested to be an underlying cause in the development of nonalcoholic fatty liver disease and/or insulin resistance. It that omega-3 acids (FA) lower DNL. We investigated effects FA supplementation on DNL and oxidation using a combination human vivo vitro studies. Research design methods Thirty-eight healthy men were randomized take either supplement (4 g/day eicosapentaenoic acid (EPA)+docosahexaenoic (DHA) as ethyl esters) or...
Disruption to sleep and circadian rhythms can impact on metabolism. The study aimed investigate the effect of acute deprivation plasma melatonin, cortisol metabolites, increase understanding metabolic pathways involved in sleep/wake regulation processes. Twelve healthy young female participants remained controlled laboratory conditions for ~92 hr with respect posture, meals environmental light (18:00-23:00 07:00-09:00 <8 lux; 23:00-07:00 0 lux (sleep opportunity) or (continuous wakefulness);...
Studies suggest that eating breakfast (as opposed to skipping breakfast) has nutrition and health benefits. However, it is not altogether clear whether these benefits are attributes of as a meal or meals include cereals. To explore associations with nutrient intakes according the type chosen, we extracted data on all foods consumed at time (0600–1000 h) from individual weighed food records respondents in National Diet Nutrition Survey adults aged 19-to-64-years. Seven days were available for...
Abstract Primary human hepatocytes are considered to be the “gold standard” cellular model for studying hepatic fatty acid and glucose metabolism; however, they come with limitations. Although HepG2 cell line retains many of primary hepatocyte metabolic functions have a malignant origin low rates triglyceride secretion. The aim this study was investigate whether dimethyl sulfoxide supplementation in media cells would enhance functionality leading development an improved vitro that closely...
Summary Total water intake is seldom reported in dietary surveys and hydration status rarely measured. Although adequate (AI) levels have been suggested by the European Food Safety Authority, World Health Organization Institute of Medicine, uncertainties confused messages abound regarding optimum intake. This paper reviews data on internationally examines associations with beverage consumption habits adults UK, as determined from secondary analysis individual diet records. On average, total...
Background and Aims Nonalcoholic fatty liver disease (NAFLD) begins with steatosis, where a mixed macrovesicular pattern of large small lipid droplets (LDs) develops. Since in vitro models recapitulating this are limited, the aims study were to develop steatosis immortalized hepatocytes investigate effects on intracellular metabolism by altering nutritional substrates. Methods Huh7 cells cultured 11 mM glucose 2% human serum (HS) for 7 days before additional sugars acids (FAs), either 200 µM...
Searchable abstracts of presentations at key conferences in endocrinology ISSN 1470-3947 (print) | 1479-6848 (online)
Searchable abstracts of presentations at key conferences in endocrinology ISSN 1470-3947 (print) | 1479-6848 (online)