A5023716166- Acute Myeloid Leukemia Research
- Acute Lymphoblastic Leukemia research
- Chronic Lymphocytic Leukemia Research
- Chronic Myeloid Leukemia Treatments
- Immune Cell Function and Interaction
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Cytomegalovirus and herpesvirus research
- T-cell and B-cell Immunology
- Immunodeficiency and Autoimmune Disorders
- Lymphoma Diagnosis and Treatment
- CAR-T cell therapy research
- Hemoglobinopathies and Related Disorders
- Single-cell and spatial transcriptomics
- Epigenetics and DNA Methylation
- Hematological disorders and diagnostics
- PI3K/AKT/mTOR signaling in cancer
- Cancer, Hypoxia, and Metabolism
- Neonatal Health and Biochemistry
- Hematopoietic Stem Cell Transplantation
- Erythrocyte Function and Pathophysiology
Aix-Marseille Université
2020-2024
Inserm
2021-2024
Centre National de la Recherche Scientifique
2021-2024
Assistance Publique Hôpitaux de Marseille
2021-2024
Hôpital de la Timone
2021-2024
Centre d’Immunologie de Marseille-Luminy
2021-2023
Hôpital Nord
2022
Acute myeloid leukaemia (AML) remains a major unmet medical, despite recent progress in targeted molecular therapies. One aspect of leukaemic cell resistance to chemotherapy is the development clones with increased capacity respond cellular stress and production reactive oxygen species (ROS), thanks particular high aldehyde dehydrogenases (ALDH) 1A1/2 activity. At diagnosis, ROS level ALDH1A1/2 activity AML patients BM are correlated different ELN 2022 prognostic groups overall survival...
Specific antigen recognition by T cell receptor (TCR) activates TCR signaling pathway, leading to proliferation and differentiation into effector memory cells. Herein, we describe protocols for stimulation assays, including procedures the isolation enrichment of mouse splenic cells ex vivo with anti-CD3/CD28 antibodies, use ovalbumin-OT-II model in stimulation. We applied this protocol show that MYC protein is essential differentiation. For complete details on execution protocol, please...
In the thymus, T cell progenitors differentiate in order to generate naive lymphocytes which migrate periphery where they will fulfill their function adaptive immune response. During thymopoiesis, genomic alterations thymocytes can promote leukemia development. Among recurrent alteration is PTEN inactivation, associated MYC overexpression. Herein, we used conditional Pten and Myc knockout mouse models single-cell RNA-sequencing approach, investigate impact of loss on physio-pathological...
PTEN (Phosphatase and TENsin homolog) is a well-known tumor suppressor involved in numerous types of cancer, including T-cell acute lymphoblastic leukemia (T-ALL). In human, loss-of-function mutations are correlated to mature T-ALL expressing receptor (TCR) at their cell surface. accordance with human T-ALL, inactivation Pten gene mouse thymocytes induces TCRαβ+ development. Herein, we explored the functional interaction between TCRαβ signaling PTEN. First, performed single-cell RNA...
Molecular analysis is the hallmark of T-cell acute lymphoblastic leukemia (T-ALL) categorization. Several T-ALL sub-groups are well recognized based on aberrant expression specific transcription factors. This recently resulted in implementation eight provisional entities into novel 2022 International Consensus Classification, albeit not updated World Health Organization classification system. Despite this extensive molecular characterization, cytogenetic remains backbone diagnosis many...
A 2.7-kg female neonate was delivered at full-term to non-consanguineous parents by caesarean section due an abnormal fetal heart tracing. During pregnancy, a routine ultrasonography screening had revealed right ventricular hypertrophy and increased nuchal translucency. After birth, the baby respiratory distress, marked hepatosplenomegaly, ecchymosis transiently red urine. Blood tests showed severe thrombocytopenia (16 × 109/l), normocytic anaemia with reticulocytosis (haemoglobin...
Multiplexed single-cell RNA-sequencing (scRNA-seq) enables investigating several biological samples in one scRNA-seq experiment. Here, we use antibodies tagged with a hashtag oligonucleotide (Ab-HTO) to label each sample, and 10× Genomics technology analyze gene expression. Advantages of sample multiplexing are reduce the cost assay avoid batch effect. It may also facilitate cell-doublet removal merging assays. Herein, apply multiplexed investigate mouse thymocytes splenic T lymphocytes...