A5077735433- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Single-cell and spatial transcriptomics
- Cytomegalovirus and herpesvirus research
- Gene Regulatory Network Analysis
- Immunodeficiency and Autoimmune Disorders
- CAR-T cell therapy research
- Sepsis Diagnosis and Treatment
- Immune Response and Inflammation
- Bioinformatics and Genomic Networks
- Acute Lymphoblastic Leukemia research
- Fungal and yeast genetics research
- Chronic Lymphocytic Leukemia Research
- PI3K/AKT/mTOR signaling in cancer
Université Claude Bernard Lyon 1
2019-2025
bioMérieux (France)
2024-2025
Hospices Civils de Lyon
2024-2025
Centre National de la Recherche Scientifique
2019-2024
Inserm
2024
Aix-Marseille Université
2024
Centre d’Immunologie de Marseille-Luminy
2021-2022
École Normale Supérieure de Lyon
2019-2020
Lyon College
2019
Specific antigen recognition by T cell receptor (TCR) activates TCR signaling pathway, leading to proliferation and differentiation into effector memory cells. Herein, we describe protocols for stimulation assays, including procedures the isolation enrichment of mouse splenic cells ex vivo with anti-CD3/CD28 antibodies, use ovalbumin-OT-II model in stimulation. We applied this protocol show that MYC protein is essential differentiation. For complete details on execution protocol, please...
In the thymus, T cell progenitors differentiate in order to generate naive lymphocytes which migrate periphery where they will fulfill their function adaptive immune response. During thymopoiesis, genomic alterations thymocytes can promote leukemia development. Among recurrent alteration is PTEN inactivation, associated MYC overexpression. Herein, we used conditional Pten and Myc knockout mouse models single-cell RNA-sequencing approach, investigate impact of loss on physio-pathological...
We present a technological advancement for the estimation of affinities Protein-Protein Interactions (PPIs) in living cells. A novel set vectors is introduced that enables quantitative yeast two-hybrid system based on fluorescent fusion proteins. The allow simultaneous quantification reaction partners (Bait and Prey) reporter at single-cell level by flow cytometry. validate applicability this small but diverse PPIs (eleven protein families from six organisms) with different affinities;...
PTEN (Phosphatase and TENsin homolog) is a well-known tumor suppressor involved in numerous types of cancer, including T-cell acute lymphoblastic leukemia (T-ALL). In human, loss-of-function mutations are correlated to mature T-ALL expressing receptor (TCR) at their cell surface. accordance with human T-ALL, inactivation Pten gene mouse thymocytes induces TCRαβ+ development. Herein, we explored the functional interaction between TCRαβ signaling PTEN. First, performed single-cell RNA...
Abstract A better understanding of sepsis-induced immunosuppression pathophysiology is desirable for the development novel therapeutic strategies to prevent and reduce rates secondary infections their associated mortality. Here we demonstrate that PD-L1 + CD44 B220 Low CD138 IgM regulatory plasma cells (PCs) are induced in a murine model immune alterations critically ill patients with bacterial sepsis COVID-19. This was revealed both by detailed analysis phenotypical features gene expression...
Multiplexed single-cell RNA-sequencing (scRNA-seq) enables investigating several biological samples in one scRNA-seq experiment. Here, we use antibodies tagged with a hashtag oligonucleotide (Ab-HTO) to label each sample, and 10× Genomics technology analyze gene expression. Advantages of sample multiplexing are reduce the cost assay avoid batch effect. It may also facilitate cell-doublet removal merging assays. Herein, apply multiplexed investigate mouse thymocytes splenic T lymphocytes...
Abstract We present a technological advancement for the estimation of affinities Protein-Protein Interactions (PPIs) in living cells. A novel set vectors is introduced that enables quantitative yeast two-hybrid system based on fluorescent fusion proteins. The allow simultaneous quantification reaction partners (Bait and Prey) reporter at single-cell level by flow cytometry. validate applicability this PPIs with different affinities. After only two hours reaction, expression can easily be...