A5049895179- Cholesterol and Lipid Metabolism
- Cellular transport and secretion
- Lipoproteins and Cardiovascular Health
- Retinal Development and Disorders
- Plant Reproductive Biology
- CRISPR and Genetic Engineering
- RNA Interference and Gene Delivery
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Cell Image Analysis Techniques
- Toxin Mechanisms and Immunotoxins
- Animal Genetics and Reproduction
- Cancer, Lipids, and Metabolism
- Cell death mechanisms and regulation
- Drug Transport and Resistance Mechanisms
University of California, Berkeley
2024
Stanford University
2020-2022
Research Network (United States)
2022
Abstract Current approaches for inserting autonomous transgenes into the genome, such as CRISPR–Cas9 or virus-based strategies, have limitations including low efficiency and high risk of untargeted genome mutagenesis. Here, we describe precise RNA-mediated insertion (PRINT), an approach site-specifically primed reverse transcription that directs transgene synthesis directly at a multicopy safe-harbor locus. PRINT uses delivery two in vitro transcribed RNAs: messenger RNA encoding avian R2...
Activating mutations in the leucine-rich repeat kinase 2 (LRRK2) cause Parkinson's disease, and previously we showed that activated LRRK2 phosphorylates a subset of Rab GTPases (Steger et al., 2017). Moreover, Golgi-associated Rab29 can recruit to surface Golgi activate it there for both auto- substrate phosphorylation. Here, define precise binding region Armadillo domain between residues 360-450 show this domain, termed 'site #1,' also bind additional substrates, Rab8A Rab10. identify...
Transport of LDL-derived cholesterol from lysosomes into the cytoplasm requires NPC1 protein; NPC1L1 mediates uptake dietary cholesterol. We introduced single disulfide bonds and to explore importance inter-domain dynamics in transport. Using a sensitive method monitor lysosomal efflux, we found that NPC1's N-terminal domain need not release rest protein for efficient export. Either introducing constrain lumenal/extracellular domains or shortening cytoplasmic loop abolishes transport...
Abstract Activating mutations in the Leucine Rich Repeat Kinase 2 (LRRK2) cause Parkinson’s disease and previously we showed that activated LRRK2 phosphorylates a subset of Rab GTPases (Steger et al., 2017). Moreover, Golgi-associated Rab29 can recruit to surface Golgi activate it there for both auto- substrate phosphorylation. Here define precise binding region Armadillo domain between residues 360-450 show this domain, termed “Site #1”, also bind additional substrates, Rab8A Rab10....
Abstract Transport of LDL-derived cholesterol from lysosomes into the cytoplasm requires NPC1 protein; NPC1L1 mediates uptake dietary cholesterol. We introduced single disulfide bonds and to explore importance inter-domain dynamics in transport. Using a sensitive method monitor lysosomal efflux, we find that NPC1’s N-terminal domain need not release rest protein for efficient export. Either introducing constrain lumenal/extracellular domains or shortening cytoplasmic loop abolishes transport...