A5046441556- Monoclonal and Polyclonal Antibodies Research
- Mast cells and histamine
- Advanced Proteomics Techniques and Applications
- Immunotherapy and Immune Responses
- vaccines and immunoinformatics approaches
- Advanced Biosensing Techniques and Applications
- Peptidase Inhibition and Analysis
- Asthma and respiratory diseases
- Immune Cell Function and Interaction
- Cellular transport and secretion
- Mass Spectrometry Techniques and Applications
- T-cell and B-cell Immunology
- Chemokine receptors and signaling
- Endoplasmic Reticulum Stress and Disease
- Autophagy in Disease and Therapy
- Lymphoma Diagnosis and Treatment
- Metabolomics and Mass Spectrometry Studies
- Cholesterol and Lipid Metabolism
- HIV Research and Treatment
- Mosquito-borne diseases and control
- Medicinal Plants and Bioactive Compounds
- Clusterin in disease pathology
- Lipid metabolism and biosynthesis
- Cell Adhesion Molecules Research
- Click Chemistry and Applications
Enzo Life Sciences (United States)
2019-2025
Stanford University
2015-2022
Stanford Medicine
2018-2019
Stratford University
2016-2017
Medicon Village
2013-2014
Lund University
2011-2014
Create (Sweden)
2011-2012
University of British Columbia
2006-2008
Uppsala University
1995-2005
Sahlgrenska University Hospital
2000
Abstract Background Accuracy in quantitative real-time RT-PCR is dependent on high quality RNA, consistent cDNA synthesis, and validated stable reference genes for data normalization. Reference used normalization impact the results generated from expression studies and, hence, should be evaluated prior to use across samples treatments. Few statistically have been reported grapevine. Moreover, success isolating RNA grapevine tissues typically limiting due low pH, polyphenolic polysaccharide...
HL is a malignant lymphoma characterized by small number of HRS cells among major population infiltrating reactive cells, e.g., lymphocytes and eosinophils. We previously reported that mast are present in HL-affected lymph nodes therein the predominant CD30L-expressing cells. The CD30L expressed on functionally active can provide stimulatory signals to Thus, constitute an important portion contribute tumor progression HL. Control recruitment this unrecognized cell its interactions with...
Abstract Mast cells are known to accumulate at sites of inflammation, however, the chemotaxins involved remain largely undefined. Transforming growth factor-β (TGF-β) isoforms regulate numerous cellular functions, including cell and differentiation, formation extracellular matrix, immune response. In this study we have compared potency different members TGF-β family as human mast chemotaxins, analyzed expression binding proteins on cells. We were able demonstrate that maximal chemotactic...
Somatic mutations in cancer are a potential source of specific neoantigens. Acute myeloid leukemia (AML) has common recurrent shared between patients addition to private individuals. We hypothesized that neoantigens derived from would be attractive targets for future immunotherapeutic approaches. Here we sought study the HLA Class I and II immunopeptidome thirteen primary AML tumor samples two cell lines (OCI-AML3 MV4-11) using mass spectrometry evaluate endogenous mutation-bearing ligands...
Performing large-scale plasma proteome profiling is challenging due to limitations imposed by lengthy preparation and instrument time. We present a fully automated multiplexed platform (AutoMP3) using the Hamilton Vantage liquid handling robot capable of preparing hundreds thousands samples. To maximize protein depth in single-shot runs, we combined 16-plex Tandem Mass Tags (TMTpro) with high-field asymmetric waveform ion mobility spectrometry (FAIMS Pro) real-time search (RTS). quantified...
Abstract Mutations in apolipoprotein L1 (APOL1) are strongly associated with an increased risk of kidney disease individuals African ancestry, yet the underlying mechanisms remain largely unknown. Plasma proteomics provides opportunities to elucidate by studying effects disease-associated variants on circulating protein levels. Here, we examine genetic drivers APOL1 and European ancestry from four independent cohorts (UK Biobank, AASK, deCODE Health ABC) employing three proteomic...
In this study we provide evidence that the chemokine stromal cell-derived factor-1alpha (SDF-1alpha) acts as a mast cell chemoattractant through interactions with its receptor CXCR4 expressed on progenitors in blood well vitro-developed and leukemic cells. We found expression of cord blood-derived cells (CBMC) human line HMC-1, analyzed by RNAse protection assay flow cytometry. SDF-1alpha induced intracellular calcium mobilization HMC-1 was chemotactic for both CBMC. The activity completely...
Transport of LDL-derived cholesterol from lysosomes into the cytoplasm requires NPC1 protein; NPC1L1 mediates uptake dietary cholesterol. We introduced single disulfide bonds and to explore importance inter-domain dynamics in transport. Using a sensitive method monitor lysosomal efflux, we found that NPC1's N-terminal domain need not release rest protein for efficient export. Either introducing constrain lumenal/extracellular domains or shortening cytoplasmic loop abolishes transport...
Bioorthogonal tools enable cell-type-specific proteomics, a prerequisite to understanding biological processes in multicellular organisms. Here we report two engineered aminoacyl-tRNA synthetases for mammalian bioorthogonal labeling: tyrosyl (ScTyrY43G) and phenylalanyl (MmPheT413G) tRNA synthetase that incorporate azide-bearing noncanonical amino acids specifically into the nascent proteomes of host cells. Azide-labeled proteins are chemoselectively tagged via azide–alkyne cycloadditions...
All nucleated mammalian cells express major histocompatibility complex (MHC) proteins that present peptides on cell surfaces for immune surveillance. These MHC-presented (pMHC) are necessary directing T-cell responses against harboring non-self antigens derived from pathogens or somatic mutations. Alterations in tumor-specific antigen repertoires — particularly novel MHC presentation of mutation-bearing (neoantigens) can be potent targets anti-tumor responses. Here we employed an integrated...
Tumor progression and prognosis in breast cancer patients are difficult to assess using current clinical laboratory parameters, where a pathological grading is indicative of tumor aggressiveness. This based on assessments nuclear grade, tubule formation, mitotic rate. We report here the first protein signatures associated with histological grades cancer, determined novel affinity proteomics approach. profiled 52 tissue samples by combining nine antibodies label-free LC-MS/MS, which generated...
The significance of the mast cell in pathogenesis rheumatic diseases has become more evident. Although hyperplasia is a feature rheumatoid arthritis, nature chemoattractants involved recruitment cells joint not been studied any detail. In this study presence chemotactic activity synovial fluids was examined.Synovial from seven patients were tested modified Boyden chamber, where human line used as responder. stem factor (SCF) and transforming growth beta (TGFbeta) measured by enzyme linked...
Major histocompatibility complex class II (MHC-II) antigen presentation underlies a wide range of immune responses in health and disease. However, how MHC-II is regulated by the peptide-loading catalyst HLA-DM (DM), its associated modulator, HLA-DO (DO), incompletely understood. This due largely to technical limitations: model antigen-presenting cell (APC) systems that express these peptidome regulators at physiologically variable levels have not been described. Likewise, computational...