A5052562434- Neuroinflammation and Neurodegeneration Mechanisms
- Long-Term Effects of COVID-19
- Single-cell and spatial transcriptomics
- Epigenetics and DNA Methylation
- Alzheimer's disease research and treatments
- Barrier Structure and Function Studies
- RNA and protein synthesis mechanisms
- Peptidase Inhibition and Analysis
- Parkinson's Disease Mechanisms and Treatments
- Nutritional Studies and Diet
- Monoclonal and Polyclonal Antibodies Research
- Tryptophan and brain disorders
- Caveolin-1 and cellular processes
- Metabolomics and Mass Spectrometry Studies
- Medicinal Plants and Bioactive Compounds
- Muscle metabolism and nutrition
- Click Chemistry and Applications
- Biotin and Related Studies
- Clusterin in disease pathology
- Neurogenesis and neuroplasticity mechanisms
- Dementia and Cognitive Impairment Research
- Drug Transport and Resistance Mechanisms
- Olfactory and Sensory Function Studies
- Infectious Encephalopathies and Encephalitis
- Adipose Tissue and Metabolism
Gladstone Institutes
2024
University of California, San Francisco
2022-2024
Stanford University
2018-2022
Abstract Animal studies show aging varies between individuals as well organs within an individual 1–4 , but whether this is true in humans and its effect on age-related diseases unknown. We utilized levels of human blood plasma proteins originating from specific to measure organ-specific differences living individuals. Using machine learning models, we analysed 11 major estimated organ age reproducibly five independent cohorts encompassing 5,676 adults across the lifespan. discovered nearly...
Recent studies indicate that the adaptive immune system plays a role in Lewy body dementia (LBD). However, mechanism regulating T cell brain homing LBD is unknown. Here, we observed cells adjacent to bodies and dopaminergic neurons postmortem brains. Single-cell RNA sequencing of cerebrospinal fluid (CSF) identified up-regulated expression C-X-C motif chemokine receptor 4 (CXCR4) CD4+ LBD. CSF protein levels CXCR4 ligand, ligand 12 (CXCL12), were associated with neuroaxonal damage...
Bioorthogonal tools enable cell-type-specific proteomics, a prerequisite to understanding biological processes in multicellular organisms. Here we report two engineered aminoacyl-tRNA synthetases for mammalian bioorthogonal labeling: tyrosyl (ScTyrY43G) and phenylalanyl (MmPheT413G) tRNA synthetase that incorporate azide-bearing noncanonical amino acids specifically into the nascent proteomes of host cells. Azide-labeled proteins are chemoselectively tagged via azide–alkyne cycloadditions...
Abstract Though SARS-CoV-2 primarily targets the respiratory system, it is increasingly appreciated that patients may suffer neurological symptoms of varied severity 1–3 . However, an unbiased understanding molecular processes across brain cell types could contribute to these in COVID-19 still missing. Here, we profile 47,678 droplet-based single-nucleus transcriptomes from frontal cortex and choroid plexus 10 non-viral, 4 COVID-19, 1 influenza patient. We complement transcriptomic data with...