A5057176925- TGF-β signaling in diseases
- Bone Tumor Diagnosis and Treatments
- Wnt/β-catenin signaling in development and cancer
- Fibroblast Growth Factor Research
- Growth Hormone and Insulin-like Growth Factors
- Cancer Cells and Metastasis
- Cancer-related gene regulation
- Cancer, Hypoxia, and Metabolism
- MicroRNA in disease regulation
- Acute Myeloid Leukemia Research
- Electrolyte and hormonal disorders
- Folate and B Vitamins Research
- Hedgehog Signaling Pathway Studies
- Ion Transport and Channel Regulation
- Thyroid Disorders and Treatments
- Neuroendocrine regulation and behavior
- Veterinary Oncology Research
- Biochemical and Molecular Research
- Adrenal and Paraganglionic Tumors
- Mechanisms of cancer metastasis
- Heat shock proteins research
- Viral Infections and Vectors
- PI3K/AKT/mTOR signaling in cancer
- Pituitary Gland Disorders and Treatments
- Herpesvirus Infections and Treatments
Utrecht University
2013-2024
Elevated basal, ligand-independent, Wnt signaling in some canine breast cancer cells is not caused by classical mutations APC, β-Catenin or GSK3β but, at least partially, enhanced LEF1 expression. We examined the expression and function of EGFR/HER-regulated pathways on ligand-independent signaling. Twelve mammary tumor cell lines with previously reported differential basal activity were used. The levels genes related to EGF-signaling analyzed cluster analysis. Cell a combined overexpression...
Heat shock proteins (HSP) are highly conserved across eukaryotic and prokaryotic species. These play a role in response to cellular stressors, protecting cells from damage facilitating recovery. In tumor cells, HSPs can have cytoprotective effects interfere with apoptotic cascades. This study was performed assess the prognostic predictive values of gene expression HSP family members canine osteosarcoma (OS) their potential for targeted therapy. Gene expressions were assessed using...
Pet dogs very frequently develop spontaneous mammary tumors and have been suggested as a good model organism for breast cancer research. In order to obtain an insight into underlying signaling mechanisms during canine tumorigenesis, in this study we assessed the incidence mechanism of canonical Wnt activation panel 12 tumor cell lines. We show that subset lines exhibit moderate activity is dependent on ligands, similar what has described human addition, three tested high not responsive...
Growth hormone (GH) and insulin‐like growth factor‐1 (IGF1) play an important role in mammalian development, cell proliferation lifespan. Especially cases of tumor there is urgent need to control the GH/IGF1 axis. In this study we screened a 38,480-compound library, two consecutive rounds analogues selection, identified active lead compounds based on following criteria: inhibition GH receptor (GHR) activity its downstream effectors Jak2 STAT5, breast colon cancer cells. The most small...
Cancer cells make extensive use of the folate cycle to sustain increased anabolic metabolism. Multiple chemotherapeutic drugs interfere with cycle, including methotrexate and 5-fluorouracil that are commonly applied for treatment leukemia colorectal cancer (CRC), respectively. Despite high success rates, therapy-induced resistance causes relapse at later disease stages. Depletion folylpolyglutamate synthetase (FPGS), which normally promotes intracellular accumulation activity natural folates...
The etiopathogenesis of feline thyrotoxicosis is unknown. transmembrane part (gene codons 480-640) the thyrotropin receptor (TSHR) gene hyperthyroid cats has already been investigated for presence stimulating mutations. No mutations were found in this TSHR gene. We have 66-530 10 and 1 euthyroid cat. This encodes as well most extracellular receptor. G(s alpha) these was also sequenced subjected to mutational analysis. Although our study revealed a polymorphism gene, no association with tumor...
Abstract Background In humans with pheochromocytomas (PCCs), targeted metabolomics is used to determine the catecholamine phenotype or uncover underlying pathogenic variants in tricarboxylic acid (TCA) cycle genes such as succinate dehydrogenase subunits ( SDHx ). Hypothesis/Objectives To analyze contents and TCA metabolites of PCCs normal adrenals (NAs). Animals Ten healthy dogs, 21 dogs PCC. Methods Prospective observational study. Dogs diagnosed PCC based on histopathological...
Canine Cushing's syndrome (hypercortisolism) can be caused by a pituitary tumor (pituitary-dependent hypercortisolism; PDH) or cortisol-secreting adrenocortical (csACT). For both cases, non-invasive biomarkers that could pre-operatively predict the risk of recurrence after surgery would greatly impact clinical decision making. The aim this study was to determine whether circulating microRNAs (miRNAs) used as diagnostic (presence PDH csACT) and/or prognostic (disease recurrence, histological...
Common disorders of water homeostasis leading to polyuria include a variety endocrine, metabolic, and renal disturbances. After exclusion most these conditions, the diagnostic dilemma differentiating between central diabetes insipidus, primary polydipsia, nephrogenic insipidus may remain. Here, we report on 18 young dogs with that had been present in cases since were puppies. The conditions categorized according plasma vasopressin (VP) response hypertonicity. VP osmotic stimulation was...
Pheochromocytomas and paragangliomas (PPGLs) are neuroendocrine tumors arising from the chromaffin cells in adrenal medulla extra-adrenal paraganglia, respectively. Local invasion, concurrent disorders, metastases prevent surgical removal, which is most effective treatment to date. Given current lack of medical treatment, there a need for novel therapeutic strategies. To identify druggable pathways driving PPGL development, we performed RNA sequencing on PPGLs (n = 19) normal medullas (NAMs;...
The present paper reports the effects of allopurinol in a raptor hyperuricaemic model. study was performed as follow-up to previous experiments wherein used doses 100 and 50 mg/kg, proved be toxic at these higher dose rates. To investigate whether 25 mg/kg (semel die) s.i.d. is safe effective Red-tailed Hawks ( Buteo jamaicensis ) reduce plasma uric acid concentrations, experimental studies were using physiologically occurring postprandial hyperuricaemia. Preprandial concentrations xanthine,...
Human epidermal growth factor 2 (HER2) overexpression leads to aggressive mammary tumour growth. Although the prognosis of HER2 + tumours in humans is greatly improved using biologicals, therapy resistance, which may be caused by increased phosphatidyl‐3‐kinase (PI3K), rous sarcoma proto‐oncogene (cSRC) or wingless‐type MMTV integration site family (Wnt) activity, a major concern. A recent analysis 12 canine cell lines showed an association between HER2/3 and phosphatase tensin homologue...
Purpose: To find underlying mutations causing highly-activated Wnt activity in mammary tumor cell lines associated with rounded morphology indicative of stemness/EMT. Methods: Stemness high was confirmed using qPCR on selected genes and microRNA profiling, followed by whole-exome sequencing 3 canine 5 low/absent lines. Candidate were identified their involvement investigated siRNA silencing. Results: The had morphological gene expression characteristics reminiscent stemness. All individual...
Common disorders of water homeostasis leading to polyuria include a variety endocrine, metabolic, and renal disturbances. After exclusion most these conditions, the diagnostic dilemma differentiating between central diabetes insipidus, primary polydipsia, nephrogenic insipidus may remain. Here, we report on 18 young dogs with that had been present in cases since were puppies. The conditions categorized according plasma vasopressin (VP) response hypertonicity. VP osmotic stimulation was...
Abstract Cancer cells make extensive use of the folate cycle to sustain increased anabolic metabolism. Multiple chemotherapeutic drugs interfere with cycle, including methotrexate and 5-fluorouracil that are commonly applied for treatment leukemia colorectal cancer (CRC), respectively. Despite high success rates, therapy-induced resistance causes relapse at later disease stages. Depletion folylpolyglutamate synthase (FPGS), which normally promotes intracellular accumulation activity both...
Abstract Canine Cushing’s syndrome (hypercortisolism) can be caused by a pituitary tumor (pituitary-dependent hypercortisolism; PDH) or cortisol-secreting adrenocortical (csACT). For both cases, noninvasive biomarkers that could pre-operatively predict the risk of recurrence after surgery would greatly impact clinical decision making. The aim this study was to determine whether circulating microRNAs (miRNAs) used as for canine syndrome. After pilot with 40 miRNAs in blood samples healthy...