Abstract Direct and rapid intracellular delivery of a functional Cas9/sgRNA complex using ultrasound‐powered nanomotors is reported. The loaded onto the nanomotor surface through reversible disulfide linkage. A 5 min ultrasound treatment enables Cas9/sgRNA‐loaded to directly penetrate plasma membrane GFP‐expressing B16F10 cells. released inside cells achieve highly effective GFP gene knockout. acoustic display more than 80 % knockout within 2 h cell incubation compared 30 static nanowires....

Vaccination represents one of the most effective means preventing infectious disease. In order to maximize utility vaccines, highly potent formulations that are easy administer and promote high patient compliance desired. present work, a biomimetic self-propelling micromotor formulation is developed for use as an oral antivirulence vaccine. The propulsion provided by magnesium-based core, cell membrane coating used detain neutralize toxic antigenic payload. resulting motor toxoids leverage...

Tremendous progress has been made during the past decade toward design of nano/micromotors with high biocompatibility, multifunctionality, and efficient propulsion in biological fluids, which collectively have led to initial investigation vivo biomedical applications these synthetic motors. Despite recent advances micromotor designs mechanistic research, significant effort is needed develop appropriate formulations micromotors facilitate their administration thus better test applicability....

Abstract Magnesium (Mg)‐based micromotors are combined with live macrophage (MΦ) cells to create a unique MΦ–Mg biohybrid motor system. The resulting biomotors possess rapid propulsion ability stemming from the Mg and biological functions provided by MΦ cell. To prepare motors, microparticles coated titanium dioxide poly( l ‐lysine) (PLL) layers incubated MΦs at low temperature. formation of such motors depends on relative size particles, swallowing up particles smaller than 5 µm....

Abstract Graphene‐based composites have received attention as part of the drive towards next‐generation electronic and energy‐storage technologies. However, current graphene synthesis methods are limited by complex, time‐consuming, toxic, costly, and/or often low‐yield procedures. The a novel stretchable graphene‐polyurethane‐poly(3,4‐ethylenedioxythiophene):polystyrene sulfonate ink aimed at printing wearable electronics is reported. procedure based on low‐cost high‐yield production...

As the most common nutritional disorder, iron deficiency represents a major public health problem with broad impacts on physical and mental development. However, treatment is often compromised by low bioavailability undesired side effects. Here, we report development of active mineral delivery vehicles using Mg-based micromotors, which can autonomously propel in gastrointestinal fluids, aiding dynamic minerals. Iron selenium are combined as model payload micromotor platform. We demonstrate...

Herein, we report ultrasound-propelled graphene-oxide coated gold nanowire motors, functionalized with fluorescein-labeled DNA aptamers (FAM-AIB1-apt), for qualitative detection of overexpressed AIB1 oncoproteins in MCF-7 breast cancer cells. The movement nanomotors under the ultrasound field facilitated intracellular uptake and resulted a faster aptamer binding target protein thus fluorescence recovery. propulsion behavior greatly enhanced intensity compared to static conditions. new...

Human papillomavirus (HPV)-associated oropharyngeal cancer (OPC) is a lethal disease with increasing incidence; however, technologies for early detection are limited. Nanomotors synthetic nanostructures that can be powered by different mechanisms and functionalized specific applications, such as biosensing. The objective of this investigation was to demonstrate an in vitro proof concept novel nanomotor-based approach toward vivo HPV-OPC.In cell line incubated ultrasound-propelled...

Abstract Direct and rapid intracellular delivery of a functional Cas9/sgRNA complex using ultrasound‐powered nanomotors is reported. The loaded onto the nanomotor surface through reversible disulfide linkage. A 5 min ultrasound treatment enables Cas9/sgRNA‐loaded to directly penetrate plasma membrane GFP‐expressing B16F10 cells. released inside cells achieve highly effective GFP gene knockout. acoustic display more than 80 % knockout within 2 h cell incubation compared 30 static nanowires....

The effect of the nature catalyst on performance and mechanism hydrogen oxidation reaction (HOR) is discussed for first time in this work. HOR an anodic that takes place anionic exchange membrane fuel cells (AEMFCs) pumps (HPs). Among investigated catalysts, Pt exhibited best HOR. However, cost availability limit usage. Co incorporated as a co-catalyst due to its oxophylic nature. Five different PtCo catalysts with loading values were synthesized order decrease loading. catalytic activities...

Synthesis of a novel cathode bimetallic Pt-Ir nanoparticles deposited on multiwall carbon nanotubes (MWCNT) by microemulsion method is reported in this paper; the composite material was dispersed with Nafion to deposit film onto Reticulated Vitreous Carbon (RVC), modified electrode used as catalyst an electrochemical reactor for H2O2 generation. The showed higher activity toward O2 reduction compared Ir/MWCNT, Pt/MWCNT and MWCNT. pathway determined. Only found lead produce H2O2. A high rate...

In this work, the synthesis of bimetallic Ag and Cu particles on carbon vulcan (AgCu/C) is reported, synthesized by a simple galvanic displacement method using citrate tribasic hydrate as co-reducing agent commercial material based Cu/C template. The materials were characterized several physicochemical techniques, including TGA, ICP-OES, XRD, SEM, BET. catalysts evaluated cathodic for oxygen reduction reaction (ORR) used preparation membrane electrode assemblies evaluation in an Anion...

Abstract Anion exchange ionomer (AEI) is a critical component used on anion membrane fuel cell (AEMFC) and alkaline water electrolyzer (AWE). In this work, quaternized polysulfone with different functionalization degree were as an to evaluate the performance in oxygen reduction reaction (ORR) hydrogen oxidation (HOR), both implied operation of AEMFC AWE. The synthesized exhibited better reactions comparison commercial AEI Aemion®. PSf‐130 performance, since IEC surface area increases twice...

Ein beschleunigter Transport von Cas9/sgRNA in Zellen beruht auf funktionalisierten Nanomotoren mit Ultraschallantrieb. In ihrer Zuschrift S. 2687 zeigen J. Wang, L. Zhang et al., dass diese akustischen schnell die Membran GFP-exprimierender durchdringen. Einmal Zelle gelangt, wird Cas9/sgRNA-Fracht unter Einwirkung der reduzierenden Umgebung das Zytosol freigesetzt, wo sie einen hoch effektiven GFP-Gen-Knockout bewirkt.

Rechargeable metal-air batteries with its high energy storage capacity and environment-friendly materials provide one of the most promising solutions as safer devices [1]. However, air cathodes rechargeable have issue irreversibility oxygen evolution reduction reactions [2]. Noble metals good bifunctional catalytic ability but are not economical sustainable. Among non-noble catalysts interest, perovskites chemical formula ABO 3 been studied extensively recently received increasing attention,...

Carbon materials are predominantly used as the support material in air electrodes due to its notable merits support, including low cost, wide availability, wettability, large surface areas, high electrical conductivity and good stability harsh environments. However, carbon bifunctional electrode is severely attacked by highly reactive oxygen generated from evolution reaction (OER) during charging [1]. Recognizing corrosion/oxidation issues with carbon-based materials, both metallic oxide...

The synthesis and characterization of bimetallic electrocatalyst was synthesized evaluated as cathode in fuel cells to ascertain their performance. main reactions that take place during the operation cell are oxidation reaction at anodic compartment oxygen reduction (ORR) cathodic compartment. Although, Pt-based materials have been widely reported catalysts both processes, high cost low availability has a strain on commercialization cells. strategy employed this work is synthesize compare...

In this paper, the simultaneous speciation of Cr(VI) and Cr(III) ionic species in aqueous solutions was achieved by measuring current a cyclic voltammogram at determined potential, under mass transport limiting conditions, using rotatory disk electrode. The influence sensor interface, rotation speed, scan rate potential on sensibility time analysis were studied. Quantification is related to oxidation process Cr(VI), while quantification associated reduction Cr(III). From voltammograms...