A5042389477- Micro and Nano Robotics
- Advanced biosensing and bioanalysis techniques
- Molecular Communication and Nanonetworks
- DNA and Nucleic Acid Chemistry
- RNA Interference and Gene Delivery
- Biosensors and Analytical Detection
- Microfluidic and Bio-sensing Technologies
- Monoclonal and Polyclonal Antibodies Research
- CRISPR and Genetic Engineering
- Advanced Biosensing Techniques and Applications
Aarhus University
2018-2022
University of California, San Diego
2018
Abstract Direct and rapid intracellular delivery of a functional Cas9/sgRNA complex using ultrasound‐powered nanomotors is reported. The loaded onto the nanomotor surface through reversible disulfide linkage. A 5 min ultrasound treatment enables Cas9/sgRNA‐loaded to directly penetrate plasma membrane GFP‐expressing B16F10 cells. released inside cells achieve highly effective GFP gene knockout. acoustic display more than 80 % knockout within 2 h cell incubation compared 30 static nanowires....
Abstract Direct and rapid intracellular delivery of a functional Cas9/sgRNA complex using ultrasound‐powered nanomotors is reported. The loaded onto the nanomotor surface through reversible disulfide linkage. A 5 min ultrasound treatment enables Cas9/sgRNA‐loaded to directly penetrate plasma membrane GFP‐expressing B16F10 cells. released inside cells achieve highly effective GFP gene knockout. acoustic display more than 80 % knockout within 2 h cell incubation compared 30 static nanowires....
Rapid detection and quantification of pharmaceutical drugs directly in human plasma is major importance for the development relevant point-of-care testing devices. Here, we report a method small molecules plasma. An assay employing molecule-linked hybridization chain reaction (HCR) has been devised pharmaceutically digoxin (Dig) methotrexate (MTX). Double modification by molecule ligands on initiator strand act as sites to control rate HCR. Upon protein binding modified strand, HCR greatly...
Homogeneous assays for determining the concentration of small molecules in biological fluids are importance monitoring blood levels critical drugs patients. We have developed a strand displacement competition assay dabigatran, methotrexate, and linezolid, which allows detection determination plasma; however, surprising kinetic behavior was observed with an initial rapid change apparent FRET values. found that protein-induced fluorescent enhancement or quenching (PIFE/Q) caused fluorescence...
The most common method for quantifying small-molecule drugs in blood samples is by liquid chromatography combination with mass spectrometry. Few immuno-based assays are available the detection of blood. Here we report on a homogeneous assay that enables concentration digoxin spiked into plasma sample. based shift equilibrium DNA strand displacement competition reaction, and can be performed 30 min concentrations above 10 nM. occurs upon binding anti-digoxigenin antibody. As model, provides...
Ein beschleunigter Transport von Cas9/sgRNA in Zellen beruht auf funktionalisierten Nanomotoren mit Ultraschallantrieb. In ihrer Zuschrift S. 2687 zeigen J. Wang, L. Zhang et al., dass diese akustischen schnell die Membran GFP-exprimierender durchdringen. Einmal Zelle gelangt, wird Cas9/sgRNA-Fracht unter Einwirkung der reduzierenden Umgebung das Zytosol freigesetzt, wo sie einen hoch effektiven GFP-Gen-Knockout bewirkt.