A5101716885- Cancer, Hypoxia, and Metabolism
- Advanced biosensing and bioanalysis techniques
- Advanced Biosensing Techniques and Applications
- Biosensors and Analytical Detection
- Epigenetics and DNA Methylation
- Cancer-related Molecular Pathways
- RNA and protein synthesis mechanisms
- Analytical Chemistry and Sensors
- Cancer Research and Treatments
- Microbial metabolism and enzyme function
- Click Chemistry and Applications
- Computational Drug Discovery Methods
- Metabolism, Diabetes, and Cancer
- Neuroscience and Neuropharmacology Research
- Synthesis and Biological Evaluation
- Gas Sensing Nanomaterials and Sensors
- Advanced Chemical Sensor Technologies
- Cellular transport and secretion
- Synthesis and characterization of novel inorganic/organometallic compounds
- DNA and Nucleic Acid Chemistry
- Calcium signaling and nucleotide metabolism
- Cancer therapeutics and mechanisms
- Organoboron and organosilicon chemistry
- Educational Research and Pedagogy
- Mechanisms of cancer metastasis
The University of Texas at Austin
2016-2025
Target (United States)
2016-2024
Ulsan National Institute of Science and Technology
2023
Austin College
2017-2020
Chung-Ang University
2016-2019
National Cancer Center
2017
Alliance for Clinical Trials in Oncology
2013
Ministry of Health and Welfare
2010
Roswell Park Comprehensive Cancer Center
2002
University at Buffalo, State University of New York
2001-2002
Aptamer biosensors have been immobilized on beads, introduced into micromachined chips the electronic tongue sensor array, and used for detection quantitation of proteins. could detect proteins in both capture sandwich assay formats. Unlike most protein-based arrays, aptamer be stripped reused multiple times. The proved to useful screening aptamers from vitro selection experiments sensitively quantitating biothreat agent ricin.
An allosteric ribozyme (aptazyme) has been used to transduce the binding of a small organic analyte (ATP) into ligation circular template for rolling circle amplification (RCA). ATP-activated deoxyribozyme ligase was immobilized on glass slide and, upon addition ATP, catalyzed padlock probe. The ligated products could be directly amplified and visualized via RCA. coupled reaction exhibited detect as little 1 μM ATP discriminate against structurally similar nucleotides such GTP, CTP, UTP....
Real-time nucleic acid amplification methods can be extremely useful for the identification and quantitation of analytes, but are more difficult to adapt protein or other analytes. To facilitate development real-time rolling circle (RCA) targets, we have developed a novel type conformation-switching aptamer that circularized upon interaction with its target, platelet-derived growth factor (PDGF). Using structure-switching aptamer, RCA used specifically quantitate PDGF down low-nanomolar...
p38 mitogen-activated protein kinases (MAPKs) participate in autophagic signaling; and previous reports suggest that pyridinyl imidazole MAPK inhibitors, including SB203580 SB202190, induce cell death some cancer cell-types through unrestrained autophagy. Subsequent studies, however, have suggested the associated cytoplasmic vacuolation resulted from off-target inhibition of an unidentified enzyme. Herein, we report SB203580-induced is rapid, reversible, relies on class III...
Abstract The hypoxia-inducible transcription factor HIF1α drives expression of many glycolytic enzymes. Here, we show that hypoxic glycolysis, in turn, increases transcriptional activity and stimulates tumor growth, revealing a novel feed-forward mechanism glycolysis-HIF1α signaling. Negative regulation by AMPK1 is bypassed cells, due to ATP elevation increased thereby preventing phosphorylation inactivation the coactivator p300. Notably, HIF1α-activated enzymes evaluated gene silencing,...
Dopamine synapses play a crucial role in volitional movement and reward-related behaviors, while dysfunction of dopamine causes various psychiatric neurological disorders. Despite this significance, the true biological nature remains poorly understood. Here, we show that transmission is strongly correlated with GABA co-transmission across brain are structured function like GABAergic marked regional heterogeneity. In addition, GABAergic-like clustered on dendrites, at has distinct...
TRIP13, a promising target for cancer therapy, has been identified as key regulator of the mitotic checkpoint. Overexpression TRIP13 is associated with poor clinical outcomes in various cancers. Inhibition potential to address therapeutic challenges cancer, particularly therapy-resistant and Rb-deficient Despite benefits inhibition, development inhibitors hindered by lack robust high-throughput screening (HTS) assay. We developed luminescence-based biochemical assay activity this challenge...
We report a new, solid-state, integrated optical array sensor platform. The and light source (OSAILS) is demonstrated for quenchometric O2 detection. OSAILS requires low voltages (3-5 V dc), it stable (< or = 5% RSD over the course of several hours continuous operation), reproducible reversible =3% RSD), exhibits rapid response time (<30 s), provides good detection limits (0.2% O2). opens door to number new strategies.
We report the first biosensor arrays based on pin printing protein-doped xerogels. The individual elements are order of 100 microm in diameter. Arrays formed (1) onto a planar substrate that is excited by an external source (laser) or (2) directly face light-emitting diode. illustrate potential our approach fabricating, testing, and characterizing four types pin-printed (PPBSA) for simultaneous detection glucose O2. analytically reliable operating ranges PPBSAs 0.1-10 mM 0.1-100% exhibit...
Extracellular signal-regulated kinases (ERK1/2) are mitogen-activated protein (MAPKs) that play a pro-tumorigenic role in numerous cancers. ERK1/2 possess two protein-docking sites distinct from the active site: D-recruitment site (DRS) and F-recruitment site. These docking facilitate substrate recognition, intracellular localization, signaling specificity, complex assembly. Targeting these on ERK therapeutic context may overcome many problems associated with traditional ATP-competitive...
Two novel bismuth oxyfluoride nitrates, Bi2OF3(NO3) and Bi6O6F5(NO3), have been synthesized via hydrothermal reactions. Whereas crystallizes in the centrosymmetric (CS) hexagonal space group, P63/m, Bi6O6F5(NO3) polar noncentrosymmetric (NCS) trigonal R3. The backbones of title compounds reveal double layered structures composed asymmetric BiF3(O/F)3 or BiO3F2 polyhedra NO3 planar groups. diffuse reflectance spectra indicate that contain wide band gaps 3.5 4.0 eV, respectively. Powder...
A new approach to rapidly produce micrometer-scale sensor elements into reusable multianalyte chemical arrays is demonstrated. By using pin printing technology in concert with sol-gel processing methods, we form discrete xerogel-based microsensors on a planar substrate. We illustrate the by forming 02- and pH-responsive sensing that allow one simultaneously determine O2 pH aqueous samples. The method allows prepare are order of 100 microm diameter, 1-2 thick, at rate approximately element...
The unstructured C-terminal domain (CTD) of eukaryotic RNA polymerase II dynamically regulates the process transcription by recruiting different factors to nascent mRNA through its multiple phosphorylation patterns. A newly discovered class phosphatases, human small phosphatases (Scp's), specifically dephosphorylates phosphorylated Ser5 (phospho.Ser5) tandem heptad repeats CTD II. Scp's also function as regulators that epigenetically silence expression specific neuronal genes, whose...
This article describes the development of a simple and robust fluorescence polarization (FP)-based binding assay adaptation to high-throughput identification small molecules blocking dsRNA NS1A protein (nonstructural 1 from type A influenza strains). homogeneous employs fluorescein-labeled 16-mer full-length tagged with glutathione S-transferase monitor changes in FP intensity simultaneously. The was optimized for screening 384-well format achieved z' score greater than 0.7. Its feasibility...
Abstract eEF‐2K is a potential target for treating cancer. However, potent specific inhibitors this enzyme are lacking. Previously, we identified 2,6‐diamino‐4‐(2‐fluorophenyl)‐4H‐thiopyran‐3,5‐dicarbonitrile (DFTD) as an inhibitor of eEF‐2K. Here describe its mechanism action against eEF‐2K, on the basis kinetic, mutational, and docking studies, use chemoinformatic approaches to identify similar class carbonitrile‐containing compounds that exhibit same action. We show DFTD behaves...
Lysyl hydroxylase-2 (LH2) catalyzes the hydroxylation of telopeptidyl lysine residues on collagen, leading to formation stable collagen cross-links that connect molecules and stabilize extracellular matrix. High levels LH2 have been reported in stabilization hydroxylysine aldehyde-derived (HLCCs), fibrosis cancer metastasis certain tissues. Identification small-molecule inhibitors targeting activity requires a robust suitable assay system, which is currently lacking. Thus, despite being...
Intracellular sensors detect changes in levels of essential metals to initiate homeostatic responses. But, a mammalian manganese (Mn) sensor is unknown, representing major gap understanding Mn homeostasis. Using human-relevant models, we recently reported that: 1) the primary response elevated upregulation hypoxia-inducible factors (HIFs), which increases expression efflux transporter SLC30A10; and 2) blocks prolyl hydroxylation HIFs by hydroxylase domain (PHD) enzymes, otherwise targets for...
eEF-2 kinase is a potential therapeutic target for breast cancer, gliomas, and depression. No potent inhibitors of eEF-2K have been reported, thus development high-throughput assay systems may expedite the process. Two assays are described using recombinant, tag-free enzyme purified from bacteria. The first fluorescence-based that uses phosphorylation Sox-based peptide substrate by eEF-2K, which results in 5-fold increase fluorescence emission, allowing continuous monitoring activity. second...