A5003423581- Microbial Metabolic Engineering and Bioproduction
- Enzyme Catalysis and Immobilization
- Fungal and yeast genetics research
- CRISPR and Genetic Engineering
- Viral Infectious Diseases and Gene Expression in Insects
- Cancer-related Molecular Pathways
- RNA and protein synthesis mechanisms
- GaN-based semiconductor devices and materials
- Synthesis and Biological Evaluation
- Gene Regulatory Network Analysis
- Plant biochemistry and biosynthesis
- Plant Gene Expression Analysis
- Cancer Diagnosis and Treatment
- Fermentation and Sensory Analysis
- Photosynthetic Processes and Mechanisms
- Biofuel production and bioconversion
- Immune Cell Function and Interaction
- Cancer Research and Treatments
The University of Texas at Austin
2007-2017
In all higher plants studied to date, the anthocyanin pigment pathway is regulated by a suite of transcription factors that include Myb, bHLH and WD-repeat proteins. However, in Arabidopsis thaliana, Myb regulators remain be conclusively identified, little known about regulation TTG1-dependent transcriptional complexes. Previous overexpression PAP1 suggested genes from entire phenylpropanoid are targets Myb/bHLH/WD-repeat complexes Arabidopsis, contrast other plants. Here we demonstrate...
Muconic acid is a valuable platform chemical with potential applications in the production of polymers such as nylon and polyethylene terephthalate (PET). The conjugate base, muconate, has been previously biosynthesized bacterial host Escherichia coli. Likewise, previous significant pathway engineering lead to first reported instance rationally engineered muconic yeast Saccharomyces cerevisiae. To further increase this host, combined adaptive laboratory evolution (ALE) strategy rational...
Gene expression requires the coordination of trans-acting factors and cis-DNA elements to initiate transcription. Here we present a coordinated approach that combines cis-acting element engineering with mutant engineer yeast promoters. Specifically, first construct hybrid promoter based on ARO9 upstream region exhibits high constitutive inducible respect exogenous tryptophan. Next, perform protein identify Aro80p affords both while retaining traits. We then use this factor drive generate...