A5102807704- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Immunodeficiency and Autoimmune Disorders
- Respiratory viral infections research
- Influenza Virus Research Studies
- Immunotherapy and Immune Responses
- Viral Infectious Diseases and Gene Expression in Insects
- Coccidia and coccidiosis research
- Toxoplasma gondii Research Studies
- SARS-CoV-2 and COVID-19 Research
- Botanical Research and Applications
- Viral gastroenteritis research and epidemiology
- bioluminescence and chemiluminescence research
- Virus-based gene therapy research
- Plant Diversity and Evolution
- Chemical synthesis and alkaloids
- Bacterial Infections and Vaccines
- Immune Response and Inflammation
- Animal Disease Management and Epidemiology
- Animal Virus Infections Studies
- Cancer Immunotherapy and Biomarkers
Wake Forest University
2020-2025
Duke University
2023-2024
High Point University
2015
Abstract T cell-independent type 2 antigens (TI-2 Ags), such as pneumococcal polysaccharides, elicit weak immunoglobulin G (IgG) responses and are refractive to boosting. Overcoming this challenge is critical for improving vaccines. Previously, we demonstrated a lipid-based adjuvant composed of monophosphoryl lipid A, synthetic cord factor, squalene significantly boosts primary secondary IgM IgG production against polysaccharide Ags. Herein, show beta-2 microglobulin, but not MHC class II,...
Current seasonal influenza virus vaccines induce responses primarily against immunodominant but highly plastic epitopes in the globular head of hemagglutinin (HA) glycoprotein. Because viral antigenic drift at these sites, need to be updated and readministered annually. To increase breadth vaccine-mediated protection, we developed an antigenically complex mixture recombinant HAs designed redirect immune more conserved domains protein. Vaccine-induced antibodies were disproportionally...
We previously reported monophosphoryl lipid A (MPL) and synthetic cord factor trehalose-6,6'-dicorynomycolate (TDCM) significantly increase Ab responses to T cell-independent type 2 Ags (TI-2 Ags) in a manner dependent on B cell-intrinsic TLR4 expression, as well MyD88 TRIF proteins. Given the capacity of MPL drive I IFN production, we aimed investigate extent which receptor (IFNAR) signaling was required for TI-2 adjuvant effects. Using Ifnar1-/- mice IFNAR1 blockade, found that IFNAR is...
ABSTRACT Seasonal influenza vaccines provide mostly strain-specific protection due to the elicitation of antibody responses focused on evolutionarily plastic antigenic sites in hemagglutinin head domain. To direct humoral response toward more conserved epitopes, we generated an virus particle where full-length protein was replaced with a membrane-anchored, “headless” variant while retaining normal complement other viral structural proteins such as neuraminidase well RNAs. We found that...
The roles distinct B cell subsets play in clonal expansion, isotype switching, and memory differentiation response to T cell-independent type 2 Ags (TI-2 Ags) has been understudied. Using sorted cells from VHB1-8 knock-in mice, we evaluated B-1b, marginal zone, follicular responses the TI-2 Ag, NP-Ficoll. All extensively divided Nonetheless, B-1b exhibited significantly increased IgG switching into Ab-secreting (ASC)-a finding that coincided with AgR signaling capacity Blimp1 expression by...
The new species Zephyranthes stellatorosea G. Lom. Sm., Spurrier, Flagg & Espejo (Amaryllidaceae) is described from Puebla and Hidalgo, Mexico, figures are included. This has affinities with Z. lindleyana Herb. macrosiphon Baker; however, distinguished by its inner perianth a basal white-starred throat, laterally compressed trilocular capsule lobes, narrower leaves.
Abstract The inability of T cell–independent type 2 (TI-2) Ags to induce recall responses is a poorly understood facet humoral immunity, yet critically important for improving vaccines. Using normal and VHB1–8 transgenic mice, we demonstrate that B cell–intrinsic PD-1 expression negatively regulates TI-2 memory cell (Bmem) generation reactivation in part through interacting with PDL1 PDL2 on non–Ag-specific cells. We also identified significant role Bmems inhibiting Ab production, thereby...
Abstract We previously described monophosphoryl lipid A (MPL) and synthetic cord factor, trehalose-6,6’-dicorynomycolate (TDCM) significantly increases antibody (Ab) responses to T cell independent type 2 antigens (TI-2 Ags) in a manner dependent on B cell-intrinsic TLR4 expression as well MyD88 TRIF adapter proteins. Given the requirement for optimal MPL/TDCM adjuvant effects capacity of MPL drive I IFN production, we aimed investigate extent which TI-2 Ab depend receptor (IFNAR) signaling....
Abstract T cell-independent type 2 antigens (TI-2 Ags), including polysaccharides present on the surface of encapsulated bacteria, are limited in their capacity to generate a recall response. The failure native polysaccharide-based vaccine-elicited antibody responses boost following either revaccination or natural infection is barrier establishing protection against infectious diseases and poorly understood facet humoral immunity. Previous work has indicated role for antigen-specific IgG...