A5073816235- Rabies epidemiology and control
- Viral Infections and Outbreaks Research
- Toxoplasma gondii Research Studies
- Viral Infections and Vectors
- Respiratory viral infections research
- Influenza Virus Research Studies
- Immune Cell Function and Interaction
- Poxvirus research and outbreaks
- Bat Biology and Ecology Studies
- Virus-based gene therapy research
- Cancer-related molecular mechanisms research
- Microbial infections and disease research
- Virology and Viral Diseases
- Cell Adhesion Molecules Research
- interferon and immune responses
- Malaria Research and Control
- bioluminescence and chemiluminescence research
- Calcium signaling and nucleotide metabolism
- Atherosclerosis and Cardiovascular Diseases
- Cytomegalovirus and herpesvirus research
- Monoclonal and Polyclonal Antibodies Research
- T-cell and B-cell Immunology
- Viral Infectious Diseases and Gene Expression in Insects
- Antimicrobial Peptides and Activities
- Mosquito-borne diseases and control
Duke University
2021-2025
Chinese Center For Disease Control and Prevention
2012-2023
Huazhong Agricultural University
2015-2022
Center of Hubei Cooperative Innovation for Emissions Trading System
2018
Alberta Hospital Edmonton
2012
Porcine deltacoronavirus (PDCoV), a member of genus Deltacoronavirus, is an emerging swine enteropathogenic coronavirus (CoV). Although outstanding efforts have led to the identification Alphacoronavirus and Betacoronavirus receptors, receptor for Deltacoronavirus unclear. Here, we compared amino acid sequences several representative CoVs. Phylogenetic analysis showed that PDCoV spike (S) protein was close cluster containing transmissible gastroenteritis virus (TGEV), which utilizes porcine...
ABSTRACT Rabies, one of the oldest infectious diseases, still presents a public health threat in most parts world today. Its pathogen, rabies virus (RABV), can utilize its viral proteins, such as nucleoprotein and phosphorylation protein, to subvert host innate immune system. For long time, large (L) protein was believed be essential for RABV transcription replication, but role pathogenicity evasion not known. Recent studies have found that conserved K-D-K-E tetrad motif L is related...
Abstract Background The central nervous system (CNS) is vulnerable to viral infection, yet few host factors in the CNS are known defend against invasion by neurotropic viruses. Long noncoding RNAs (lncRNAs) have been revealed play critical roles a wide variety of biological processes and highly abundant mammalian brain, but their defending pathogens into remain unclear. Results We report here that multiple viruses, including rabies virus, vesicular stomatitis Semliki Forest herpes simplex...
One approach for developing a more universal influenza vaccine is to elicit strong immune responses against canonically immunosubdominant epitopes in the surface exposed viral glycoproteins. While standard vaccines typically induce directed primarily mutable hemagglutinin (HA) head domain, there are generally limited or variable relatively conserved HA stalk domain and neuraminidase (NA) proteins. Here we describe that utilizes combination of wildtype (WT) virus particles along with...
Rabies is an ancient disease but remains endemic in most parts of the world and causes approximately 59,000 deaths annually. The mechanism through which causative agent, rabies virus (RABV), evades host immune response infects central nervous system (CNS) has not been completely elucidated thus far. Our previous studies have shown that lab-attenuated, wild-type (wt), RABV activates innate mouse dog models. In this present study, we demonstrate lab-attenuated abortive infection astrocytes,...
Rabies, caused by rabies virus (RABV), is an acute, fatal encephalitic disease that affects many warm-blooded mammals. Currently, post-exposure prophylaxis regimens are effective for most cases, but once the clinical signs of appear, current treatment options become ineffective. Carrageenan has been reported as a potent inhibitor viruses. In this study, λ-carrageenan (λ-CG) P32 was investigated its potential role in inhibiting RABV infection. Our results show specifically inhibits...
ABSTRACT Rabies continues to present a public health threat in most countries of the world. The efficient way prevent and control rabies is implement vaccination programs for domestic animals. However, traditional inactivated vaccines used animals are costly have relatively low efficiency, which impedes their extensive use developing countries. There is, therefore, an urgent need develop single-dose long-lasting vaccines. little information available regarding mechanisms underlying...
Rabies, caused by rabies virus (RABV), is a fatal neurological disease that still causes more than 59,000 human deaths each year. Type III interferon IFN-λs are cytokines with type I IFN-like antiviral activities. Although IFN-λ can restrict the infection for some viruses, especially intestinal inhibitory effect against RABV remains undefined. In this study, function of IFN was investigated. Initially, we found IFN-λ2 and IFN-λ3 could inhibit replication in cells. To characterize role mouse...
Abstract Background Gut microbiome plays a crucial role in modulating human and animal immune responses. Rabies is fatal zoonosis causing encephalitis mammals vaccination the most effective method to control eliminate rabies. The relationship between gut humoral immunity post rabies has not been investigated yet. Methods Mice orally administrated with cocktail of broad‐spectrum antibiotics were inoculated vaccines, response was analyzed at indicated time points. 16S ribosomal RNA (16S rRNA)...
Rabies virus (RABV) causes fatal encephalitis in mammals and poses a public health threat many parts of the world. Vaccination remains most effective means for prevention control rabies. Studies focusing on mechanism RABV immunogenicity are necessary improvement rabies vaccines. Toll-like receptor 7 (TLR7), an innate sensing single-stranded viral RNA, is important induction adaptive immunity. Our studies revealed that absence TLR7 led to lower antibody production mice immunized with RABV. It...
Tripartite motif-containing protein 25 (Trim25) is an E3 ubiquitin ligase that activates retinoid acid-inducible gene I (RIG-I) and promotes the antiviral interferon response. Recent studies have shown Trim25 can bind degrade viral proteins, suggesting a different mechanism of on its effects. In this study, expression was upregulated in cells mouse brains after rabies virus (RABV) infection. Moreover, limited RABV replication cultured cells. Overexpression caused attenuated pathogenicity...
Current seasonal influenza virus vaccines induce responses primarily against immunodominant but highly plastic epitopes in the globular head of hemagglutinin (HA) glycoprotein. Because viral antigenic drift at these sites, need to be updated and readministered annually. To increase breadth vaccine-mediated protection, we developed an antigenically complex mixture recombinant HAs designed redirect immune more conserved domains protein. Vaccine-induced antibodies were disproportionally...
Interferon and its downstream products, ISGs, are essential in defending against pathogen invasion. One of the IIGP1, has been found to constrain intracellular parasite infection by disrupting their vacuole membranes. However, role IIGP1 limiting viral is unclear. In this study, we show that with a typical neurotropic virus, RABV, can induce upregulation which, turn, suppresses RABV interacting phosphoprotein (P protein) thus blocking dimerization P protein. Our study provides first evidence...
Rabies, caused by rabies virus (RABV), is a fatal encephalitis in humans and other mammals, which continues to present public health threat most parts of the world. Our previous study demonstrated that Toll-like receptor 7 (TLR7) essential induction anti-RABV antibodies via facilitation germinal center formation. In study, we investigated role TLR7 pathogenicity RABV mouse model. Using isolated plasmacytoid dendritic cells (pDCs), an innate recognition for RABV. When invaded from periphery,...
ABSTRACT Seasonal influenza vaccines provide mostly strain-specific protection due to the elicitation of antibody responses focused on evolutionarily plastic antigenic sites in hemagglutinin head domain. To direct humoral response toward more conserved epitopes, we generated an virus particle where full-length protein was replaced with a membrane-anchored, “headless” variant while retaining normal complement other viral structural proteins such as neuraminidase well RNAs. We found that...
Rabies, caused by rabies virus (RABV), is a fatal zoonosis, which still poses threat to public health in most parts of the world. Glycoprotein RABV only viral surface protein, critical for induction virus-neutralizing antibodies (VNA). In order improve production VNA, recombinant RABVs containing two copies G gene and codon-optimized were constructed using reverse genetics, named LBNSE-dG LBNSE-dOG, respectively. After being inoculated into mouse brains, LBNSE-dOG induced more apoptosis...
Rabies, caused by the rabies virus (RABV), remains a serious threat to public health in most countries. Development of single-dose and efficacious vaccine is important method restrict transmission. Costimulatory factor OX40-ligand (OX40L) plays crucial role T cell-dependent humoral immune responses through T-B cell interaction. In this work, recombinant RABV overexpressing mouse OX40L (LBNSE-OX40L) was constructed, its effects on immunogenicity were evaluated model. LBNSE-OX40L-immunized...
Rabies is a zoonotic disease caused by the rabies virus (RABV). RABV can lead to fatal encephalitis and still serious threat in most parts of world. Interferon regulatory factor 7 (IRF7) main transcriptional regulator type I IFN, it crucial for induction IFNα/β IFN-dependent immune response. In this study, we focused on role IRF7 pathogenicity immunogenicity using an -/- mouse model. The results showed that absence made mice more susceptible RABV, because restricted replication early stage...
Influenza virus vaccines currently afford short-term protection from viruses that are closely related to the vaccine strains. There is much effort develop improved, next-generation influenza elicit broader and longer-lasting protection.
Abstract The central nervous system (CNS) is vulnerable for viral infection, yet few host factors in the CNS are known to defend invasion by neurotropic viruses. We report here that multiple viruses, including rabies virus (RABV), vesicular stomatitis (VSV), Semliki Forest (SFV) and herpes simplex 1 (HSV-1), elicit neuronal expression of a host-encoded lncRNA EDAL. EDAL inhibits replication these viruses cells RABV infection mouse brains. binds conserved histone methyltransferase enhancer...
To investigate the awareness of malaria-related knowledge, use mosquito nets and their influencing factors among residents in Banlao Township, Cangyuan County, Yunnan Province.In August 2020, 19 settlement sites Lincang City, Province were selected as study areas, permanent at ages 10 years older enrolled for a questionnaire survey, including residents' demographics, family economic status, malaria control knowledge nets. In addition, affecting night prior to survey identified using...
Background Natural Killer (NK) cells play a crucial role in ADCC response through FcgRIIIa (CD16), which is able to bind Fc region of IgG displayed on target cells. The presence another class low-affinity receptor for IgG, called FcgRII(CD32), has also been reported human. It raises the possibility CD32 expression NK non-human primate models. Efforts get clearer understanding marker might be related function rhesus macaques will helpful evaluation vaccines nonhuman