A5113453529- Metabolism, Diabetes, and Cancer
- Adipose Tissue and Metabolism
- Immunotherapy and Immune Responses
- Monoclonal and Polyclonal Antibodies Research
- Muscle Physiology and Disorders
- Pancreatic function and diabetes
- Immune Cell Function and Interaction
- Glycosylation and Glycoproteins Research
- Mitochondrial Function and Pathology
- Muscle metabolism and nutrition
- Diet and metabolism studies
- Macrophage Migration Inhibitory Factor
- Cancer Immunotherapy and Biomarkers
- RNA Interference and Gene Delivery
- Neuroscience and Neuropharmacology Research
- Cancer Research and Treatments
- Exercise and Physiological Responses
- Phagocytosis and Immune Regulation
- Pancreatic and Hepatic Oncology Research
- Asthma and respiratory diseases
- Receptor Mechanisms and Signaling
- Pneumocystis jirovecii pneumonia detection and treatment
- Radiopharmaceutical Chemistry and Applications
- T-cell and B-cell Immunology
- Genetics and Neurodevelopmental Disorders
Brigham Young University
2014-2025
University of Strathclyde
2010-2023
Montreal General Hospital
1985-2019
McGill University
1982-2019
Edinburgh Royal Infirmary
2015
University of Edinburgh
2015
University of Glasgow
2010
East Carolina University
2005-2008
Piedmont International University
2006
H.T. Harvey & Associates
2001
A radioimmunoassay has been developed for determining the serum levels of carcinoembryonic antigen human digestive system in patients with cancer colon and rectum. The assay is simple to perform a high degree reproducibility specificity. test detects concentration 2.5 ng per ml this provided first demonstration circulating tumor-specific sera patients.
AMP-activated protein kinase (AMPK) has been identified as a regulator of gene transcription, increasing mitochondrial proteins oxidative metabolism well hexokinase expression in skeletal muscle. In mice, muscle-specific knockout LKB1, component the upstream AMPK, prevents contraction- and 5-aminoimidazole-4-carboxamide-1-beta-D-ribofuranoside (AICAR)-induced activation AMPK muscle, increase II that is normally observed with chronic AICAR AMPK. Since previous reports show cAMP response...
Regulation of protein translation through Akt and the downstream mammalian target rapamycin (mTOR) pathway is an important component cellular response to hypertrophic stimuli. It has been proposed that 5'-AMP-activated kinase (AMPK) activation during muscle contraction may limit resistance-type exercise by inhibiting translational signaling. However, experimental manipulation AMPK activity such a stimulus not attempted. Therefore, we investigated whether can attenuate signaling Akt/mTOR...
A unique property of skeletal muscle is its ability to adapt mass changes in activity. Inactivity, as disuse or aging, causes atrophy, the loss and strength, leading physical incapacity poor quality life. Here, through a combination transcriptomics transgenesis, we identify sestrins, family stress-inducible metabolic regulators, protective factors against wasting. Sestrin expression decreases during inactivity genetic deficiency exacerbates wasting; conversely, sestrin overexpression...
14-3-3ζ promotes cell survival via dynamic interactions with a vast network of binding partners, many which are involved in stress regulation. We show here that hypoxia (low glucose and oxygen) triggers rearrangement the interactome to favor an interaction core autophagy regulator Atg9A. Our data suggest localization mammalian Atg9A autophagosomes requires phosphorylation on C terminus at S761, creates docking site. Under basal conditions, this is maintained low level dependent both ULK1...
LKB1 has been identified as a component of the major upstream kinase AMP-activated protein (AMPK) in skeletal muscle. To investigate roles muscle, we used muscle-specific knockout (MLKB1KO) mice that exhibit low expression heart and but not other tissues. The importance muscle physiology was demonstrated by observation electrical stimulation situ increased AMPK phosphorylation activity wild-type (WT) LKB1KO mice. Likewise, acetyl-CoA carboxylase (ACC) markedly attenuated KO had difficulty...
Skeletal muscle mass declines with age, as does the potential for overload-induced fast-twitch skeletal hypertrophy. Because 5′-AMP-activated protein kinase (AMPK) activity is thought to inhibit synthesis and may therefore modulate hypertrophy, purpose of this investigation was examine AMPK phosphorylation status (a marker activity) its association attenuated hypertrophy observed in aged muscle. One-week overload plantaris slow-twitch soleus muscles achieved young adult (8 mo; n = 7) old (30...
Abstract The peripheral blood monocyte is the reactive cell in tube LAI assay. loses its properties of adherence to glass upon exposure specific antigen. Two different experiments determine if lymphocytes, when they reacted with tumour, released mediators that were responsible for inhibiting adherence, gave negative results. mechanism whereby tumour antigen appeared be recognized was binding cytophilic IgG anti‐tumour antibody receptors on surface monocyte. results indicate normal monocytes...
Lipid metabolism is important for health and insulin action, yet the fundamental process of regulating lipid during muscle contraction incompletely understood. Here, we show that liver kinase B1 (LKB1) muscle-specific knockout (LKB1 MKO) mice display decreased fatty acid (FA) oxidation treadmill exercise. LKB1 MKO also SIK3 activity, increased histone deacetylase 4 expression, NAD+ concentration SIRT1 expression genes involved in FA oxidation. In AMP-activated protein (AMPK)α2 KO mice,...
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Impaired overload-induced protein synthesis and growth in aged fast-twitch skeletal muscle may result from diminished responsiveness of signalling intermediates controlling translation. Yet, potential age-related decrements have never been examined direct parallel with impaired any model. To this end, we used Western blotting to examine the contents phosphorylation states mammalian target rapamycin (mTOR) its downstream translational intermediates, 70 kDa ribosomal S6 kinase (S6k), (rpS6),...
Abstract Leukocytes from patients with limited cancer display LAI reactivity whereas leukocytes metastatic frequently demonstrate no in the tube assay. The (monocytes) of reactive react tumour antigen through specific cytophilic anti‐tumour IgG antibody bound to monocyte's Fc cell surface receptors. non‐reactive monocytes advanced lacked ability bind free antibody. Moreover, serum patient contained capable “arming” normal leukocytes. large burdens antigenic determinants absorbing or when...
Abstract Peripheral blood leukocytes (PBL) from breast cancer patients with early or localized fail to adhere glass in the presence of extract. The same do not react unrelated tumour extracts. Enrichment and depletion certain PBL populations apparently indicated that indicator and/or reactive cell manifesting non‐adherence appropriate antigen was phagocytic, adherent absence had surface Fc‐receptors. involved, therefore, appears be circulating monocyte. These results show tube LAI assay,...