A5071932044- Protein purification and stability
- Monoclonal and Polyclonal Antibodies Research
- Viral Infectious Diseases and Gene Expression in Insects
- Thermodynamic properties of mixtures
- Microfluidic and Capillary Electrophoresis Applications
- Phase Equilibria and Thermodynamics
- Microfluidic and Bio-sensing Technologies
- Analytical Chemistry and Chromatography
- Process Optimization and Integration
- Crystallization and Solubility Studies
- Chemical Thermodynamics and Molecular Structure
- Chemical and Physical Properties in Aqueous Solutions
University of Lisbon
2012-2019
Institute for Biotechnology and Bioengineering
2014-2019
Multimodal ligands are synthetic molecules comprising multiple types of interactions that have been increasingly used for the capture different biopharmaceutical compounds within complex biological mixtures. For monoclonal antibodies (mAbs) in particular, these shown possibility direct from cell culture supernatants native conditions, as well enhanced selectivity and affinity compared to traditional single-mode ligands. However, performing a target mAb using multimodal chromatography comes...
Abstract BACKGROUND The emergence of monoclonal antibodies ( mAbs ) as new biopharmaceutical products requires the development purification methods that are not only effective but able to reduce production costs. To address problematic recovery , gum arabic GA coated magnetic particles MPs were used for human from animal cells supernatants. RESULTS synthesized via co‐precipitation and exhibited a spherical‐like physical aspect, with an average hydrodynamic diameter 473 nm zeta potential –26...
Phenylboronate chromatography has been employed for bioseparation applications though details concerning the mechanisms of interaction between ligand and macromolecules remain widely unknown. Here, phenomena underlying adsorption an anti‐human interleukin‐8 (anti‐IL8) monoclonal antibody (mAb) onto m ‐aminophenylboronic acid ( ‐APBA) in presence different mobile‐phase modulators (NaF/MgCl 2 /(NH 4 ) SO under pH values (7.5/8.5/9.0) is investigated. Flow microcalorimetry (FMC) applied to...