A5028976604- Microbial Natural Products and Biosynthesis
- Glycosylation and Glycoproteins Research
- Plant biochemistry and biosynthesis
- Plant Gene Expression Analysis
- Microbial Metabolic Engineering and Bioproduction
- Microbial Metabolites in Food Biotechnology
- Carbohydrate Chemistry and Synthesis
- Cancer Treatment and Pharmacology
- Enzyme Structure and Function
- Natural product bioactivities and synthesis
- Amino Acid Enzymes and Metabolism
- Enzyme Catalysis and Immobilization
- Streptococcal Infections and Treatments
- Neonatal and Maternal Infections
- Diet, Metabolism, and Disease
- Pharmacological Effects of Natural Compounds
- Phytochemistry and Bioactive Compounds
- Plant tissue culture and regeneration
- Chromatography in Natural Products
- Plant nutrient uptake and metabolism
- Steroid Chemistry and Biochemistry
- Microbial Metabolism and Applications
- Phytochemical compounds biological activities
- Sirtuins and Resveratrol in Medicine
- Flavonoids in Medical Research
University of Kentucky
2021-2022
Sun Moon University
2013-2019
Government of the Republic of Korea
2017
ABSTRACT A UDP glucosyltransferase from Bacillus licheniformis was overexpressed, purified, and incubated with nucleotide diphosphate (NDP) d - l -sugars to produce glucose, galactose, 2-deoxyglucose, viosamine, rhamnose, fucose sugar-conjugated resveratrol glycosides. Significantly higher (90%) bioconversion of achieved α- -glucose as the sugar donor four different glucosides resveratrol: 3- O -β- -glucoside, 4′- 3,5- -diglucoside, 3,5,4′- -triglucoside. The conversion rates numbers...
A multi-monocistronic synthetic vector was used to assemble multiple genes of a nucleotide diphosphate (NDP)-sugar biosynthetic pathway construct robust genetic circuits for the production valuable flavonoid glycosides in Escherichia coli. Characterized functional involved biosynthesis uridine (UDP)-glucose and thymidine (TDP)-rhamnose from various microbial sources along with glucose facilitator diffusion protein (glf) glucokinase (glk) Zymomonas mobilis were assembled overexpressed single...
ABSTRACT The glycosylation of five different flavonols, fisetin, quercetin, myricetin, kaempferol, and 3-hydroxyflavone, was achieved by applying YjiC. 3-Hydroxyflavone selected as a probe for in vitro glycorandomization all flavonols using diverse nucleotide diphosphate- d/l -sugars. This study unlocked the possibilities glycodiversification generation novel compounds future therapeutics.
Multi-monocistronic and multi-variate vectors were designed, built, tested for the improved production of cyanidin 3-O-glucoside (C3G) in Escherichia coli BL21 (DE3). The synthetic bio-parts designed such a way that multiple genes can be assembled using bio-brick system, expressed under different promoters single vector. harbor compatible cloning sites, so shuffled from one vector to another step, into two required genes: anthocyanidin synthase (PhANS) Petunia hybrida,...
Epothilones are extremely cytotoxic chemotherapeutic agents with epoxide, thiazole, and ketone groups that share equipotent kinetic similarity taxol. The in vitro glycosylation catalyzed by uridine diphosphate glucosyltransferase (YjiC) from Bacillus licheniformis generated six novel epothilone A glycoside analouges including 7-O-β-D-glucoside, 7-O-β-D-galactoside, 3,7-O-β-D-digalactoside, 7-O-β-D-2-deoxyglucoside, 7-O-β-L-rhamnoside, 7-O-β-L-fucoside. Epothilone 7-O-β-D-glucoside was...
Various flavonoid derivatives including methoxylated flavones display remarkable biological activities. Chrysoeriol is a flavone of great scientific interest because its promising anti-microbial activities against various Gram-negative and Gram-positive bacteria. Sustainable production such compounds therefore pronounced to biotechnologists in the pharmaceutical nutraceutical industries. Here, we used sugar O-methyltransferase enzyme from spinosyn biosynthesis gene cluster Saccharopolyspora...
Abstract The cell wall of the human bacterial pathogen Group A Streptococcus (GAS) consists peptidoglycan decorated with Lancefield group carbohydrate (GAC). GAC is a promising target for development GAS vaccines. In this study, employing chemical, compositional, and NMR methods, we show that attached to via glucosamine 1-phosphate. This structural feature makes GAC-peptidoglycan linkage highly sensitive cleavage by nitrous acid resistant mild conditions. Using characteristic wall, identify...
Two promiscuous Bacillus licheniformis glycosyltransferases, YdhE and YojK, exhibited prominent stereospecific but nonregiospecific glycosylation activity of 20 different classes 59 structurally natural non-natural products. Both enzymes transferred various sugars at three nucleophilic groups (OH, NH2, SH) diverse compounds to produce O-, N-, S-glycosides. The also displayed a catalytic reversibility potential for one-pot transglycosylation, thus bestowing cost-effective application in...
Microbial cell factories are extensively used for the biosynthesis of value-added chemicals, biopharmaceuticals, and biofuels. is also realistic production heterologous molecules including complex natural products plant microbial origin. Glycosylation a well-known post-modification method to engineer sugar-functionalized products. It particular interest chemical biologists increase diversity molecules. Employing state-of-the-art systems synthetic biology tools, range small glycosylated have...
Biosynthesis of various genistein glycopyranoside scaffolds using versatile GTs and SOMTs. Each compound was structurally characterized biological activity assay carried out.