A5052839519- Microbial Natural Products and Biosynthesis
- Carbohydrate Chemistry and Synthesis
- Synthetic Organic Chemistry Methods
- Genomics and Phylogenetic Studies
- Biochemical and Molecular Research
- Chemical Synthesis and Analysis
- Global trade and economics
- Signaling Pathways in Disease
- RNA and protein synthesis mechanisms
- Enzyme Catalysis and Immobilization
- Marine Sponges and Natural Products
- Plant biochemistry and biosynthesis
- Peptidase Inhibition and Analysis
- Microbial Metabolic Engineering and Bioproduction
- Glycosylation and Glycoproteins Research
- Fungal Biology and Applications
- Molecular Sensors and Ion Detection
- Phytochemical compounds biological activities
- Cancer therapeutics and mechanisms
- Enzyme Production and Characterization
- Microbial Metabolism and Applications
- Fiscal Policy and Economic Growth
- Plant Disease Resistance and Genetics
- Luminescence and Fluorescent Materials
- Viral Infectious Diseases and Gene Expression in Insects
Seoul National University
1997-2025
Pusan National University
2015-2024
New Generation University College
2022
Korea Institute for International Economic Policy
2019-2021
Seoul Institute
2019-2021
Government of the Republic of Korea
2014-2021
Ewha Womans University
2011-2020
Ewha Womans University Medical Center
2007-2019
University of Minnesota
2002-2018
Twin Cities Orthopedics
2018
New 4,4-difluoro-4-bora-3a,4a-diaza-s-indacene (BODIPY) derivatives (1 and 2) were synthesized as an "off−on" fluorescent chemosensor chemodosimeter for Cu2+ Pb2+. Compound 1 displayed selective large chelation enhanced fluorescence effects with Pb2+ among the metal ions examined. On other hand, compound 2, a chemodosimeter, effectively recognized via hydrolysis of acetyl group.
Abstract Individual Streptomyces species have the genetic potential to produce a diverse array of natural products commercial, medical and veterinary interest. However, these are often not detectable under laboratory culture conditions. To harness their full biosynthetic potential, it is important develop detailed understanding regulatory networks that orchestrate metabolism. Here we integrate nucleotide resolution genome-scale measurements transcriptome translatome coelicolor , model...
The allyl moiety of the immunosuppressive agent FK506 is structurally unique among polyketides and critical for its potent biological activity. Here, we detail biosynthetic pathway to allylmalonyl-coenzyme A (CoA), from which group derived, based on a comprehensive chemical, biochemical, genetic interrogation three gene clusters. discrete polyketide synthase (PKS) with noncanonical domain architecture presumably in coordination fatty acid host catalyzes multistep enzymatic reaction...
A new fluorescent chemosensor based on the acridine-Zn(II) derivative effectively recognizes pyrophosphate and inorganic phosphate at pH 7.4. Acridine 1 displayed a quenching effect with pyrophosphate; other hand, large enhancement was observed phosphate. [structure: see text].
The logical and effective discovery of macrolactams, structurally unique natural molecules with diverse biological activities, has been limited by a lack targeted search methods. Herein, method for macrolactams was devised coupling genomic signature-based PCR screening bacterial DNA library spectroscopic early identification macrolactams. facilitated the efficient selection 43 potential macrolactam-producing strains (3.6% 1,188 screened). amplicons amine-deprotecting enzyme-coding genes were...
[structure: see text] A new cavitand bearing four imidazolium groups was synthesized for the recognition of anions through (C-H)+...X- hydrogen bond formation. The binding properties toward various including dicarboxylates were examined on basis 1H NMR spectroscopic experiments.
Heterologous expression of the barbamide biosynthetic gene cluster, obtained from marine cyanobacterium Moorea producens, in terrestrial actinobacterium Streptomyces venezuelae, resulted production a new congener 4-O-demethylbarbamide, demonstrating potential this approach for investigating assembly and tailoring complex natural products.
A biocatalytic platform that employs the final two monomodular type I polyketide synthases of pikromycin pathway in vitro followed by direct appendage D-desosamine and C-H oxidation(s) vivo was developed applied toward synthesis a suite 12- 14-membered ring macrolide natural products. This methodology delivered both compound classes 13 steps (longest linear sequence) from commercially available (R)-Roche ester >10% overall yields.
FK506 (tacrolimus) is an FDA-approved immunosuppressant indicated for the prevention of allograft rejections in patients undergoing organ transplants. In mammals, inhibits calcineurin-nuclear factor activated T cells (NFAT) pathway to prevent T-cell proliferation by forming a ternary complex with its binding protein, FKBP12, and calcineurin. also exerts antifungal activity inhibiting calcineurin, which essential virulence human-pathogenic fungi. Nevertheless, cannot be used directly as drug...
Abstract Quantitative measurement of protein–protein interactions (PPIs) within living cells is vital for understanding their cellular functions at the molecular level and applications in synthetic biology, protein engineering, drug discovery. Although several techniques have been developed to measure PPI strength vitro, direct bacterial remains challenging. Here, a method quantitatively measuring PPIs by determining dissociation constant ( K d ) E. coli using fluorescence resonance energy...